Abstract
Two genotypes of Brassica napus species (Talayeh and RGS003) and the explants segment (hypocotyls and cotyledon) were tested for their potential to produce somatic embryos in in vitro condition. The effect of genotype, different explants and also different concentrations of plant growth regulators (PGRs) including: -naphthalene acetic acid (NAA), 2,4-Dichlorophenoxy acetic acid (2,4-D), 6-benzyl amino purine (BAP), were investigated. The basal medium was Murashige and Skoog (MS) supplemented with 2% sucrose and 1% agar. Explants plated on different callus induction medium and each 4 week was subculture in the same medium. The results showed that ‘Talayeh’, in terms of regeneration, had significant difference with RGS003 and produce more somatic embryos. In this genotype, modified MS medium containing 2% sucrose, 6 mg l-1 sodium chloride (NaCl), 3 mg l-1 BAP, 2 mg l-1 NAA and 2 mg l-1 2,4-D was the most efficient medium for somatic embryogenesis in ‘Talayeh’ and also, hypocotyl explants were found to be more suitable than cotyledon for somatic embryogenesis.
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Claims 4
Claim · model-written summary
Talayeh had a significantly different regeneration from RGS003 in in vitro culture.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Material
- Talayehe0
- Outcome
- regeneratione1
- Comparator
- RGS003e1
- Setting
- in vitro conditione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Two genotypes of Brassica napus species (Talayeh and RGS003) and the explants segment (hypocotyls and cotyledon) were tested for their potential to produce somatic embryos in in vitro condition”
e1“in terms of regeneration, had significant difference with RGS003 and produce more somatic embryos”
Claim · model-written summary
Talayeh produced more somatic embryos than RGS003 in in vitro culture.
- Taxon
- Brassica napusBrassica napuse0
- Material
- Talayehe0
- Outcome
- somatic embryose1
- Direction
- moree1
- Comparator
- RGS003e1
- Setting
- in vitro conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two genotypes of Brassica napus species (Talayeh and RGS003) and the explants segment (hypocotyls and cotyledon) were tested for their potential to produce somatic embryos in in vitro condition”
e1“in terms of regeneration, had significant difference with RGS003 and produce more somatic embryos”
Claim · model-written summary
Hypocotyl explants were more suitable than cotyledon explants for somatic embryogenesis of rapeseed.
- Taxon
- Brassica napusBrassica napuse0
- Organ / stage
- hypocotyl explantse2
- Treatment
- hypocotyl explantse2
- Outcome
- somatic embryogenesise2
- Direction
- more suitablee2
- Comparator
- cotyledone2
- Setting
- in vitro conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two genotypes of Brassica napus species (Talayeh and RGS003) and the explants segment (hypocotyls and cotyledon) were tested for their potential to produce somatic embryos in in vitro condition”
e2“hypocotyl explants were found to be more suitable than cotyledon for somatic embryogenesis”
Claim · model-written summary
The MS medium with 2% sucrose, 6 mg l-1 NaCl, 3 mg l-1 BAP, 2 mg l-1 NAA and 2 mg l-1 2,4-D was the most efficient medium for somatic embryogenesis of Talayeh.
- Taxon
- Brassica napusBrassica napuse0
- Material
- Talayehe0
- Treatment
- modified MS medium containing 2% sucrose, 6 mg l-1 sodium chloride (NaCl), 3 mg l-1 BAP, 2 mg l-1 NAA and 2 mg l-1 2,4-De3
- Outcome
- somatic embryogenesise3
- Direction
- most efficiente3
- Setting
- in vitro conditione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two genotypes of Brassica napus species (Talayeh and RGS003) and the explants segment (hypocotyls and cotyledon) were tested for their potential to produce somatic embryos in in vitro condition”
e3“modified MS medium containing 2% sucrose, 6 mg l-1 sodium chloride (NaCl), 3 mg l-1 BAP, 2 mg l-1 NAA and 2 mg l-1 2,4-D was the most efficient medium for somatic embryogenesis”
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