Abstract
The effect of naphthaleneacetic acid (NAA), an auxin, and N 6 -benzyladenine (BA), a cytokinin, on microspore embryogenesis in two F 1 (hybrids of rapeseed (Brassica napus L.) was investigated, using a two-factor, central-composite response surface design. Total embryo yields of both hybrids increased in a log-linear fashion with BA concentrations in the range 0.01–0.255 mg L −1 ; within this range, yields approximately doubled for every fivefold increase in BA concentration. NAA concentrations of 0.136–1.85 mg L −1 had no effect on embryo yields. Within the range of concentrations studied, neither growth substance had any effect on the proportion of cotyledonary embryos produced. When cotyledonary embryos were regenerated on B5 medium without hormones, no significant effects on either root development or shoot production attributable to the NAA–BA treatments could be detected. In general, higher embryo yields were associated with a slower rate of development, but a greater degree of morphological synchrony within individual cultures.
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Claims 7
Claim · model-written summary
BA increased total embryo yields in both hybrids, approximately doubling with each fivefold BA increase.
Verbatim
e0“Total embryo yields of both hybrids increased in a log-linear fashion with BA concentrations in the range 0.01–0.255 mg L −1 ; within this range, yields approximately doubled for every fivefold increase in BA concentration”
t0“rapeseed”
Claim · model-written summary
NAA at 0.136–1.85 mg L−1 had no effect on embryo yields.
- Taxon
- rapeseedt0
- Treatment
- NAA concentrations of 0.136–1.85 mg L −1e0
- Outcome
- embryo yieldse0
- Direction
- no effecte0
Not reportedComparator
Verbatim
e0“NAA concentrations of 0.136–1.85 mg L −1 had no effect on embryo yields”
t0“rapeseed”
Claim · model-written summary
Neither growth substance affected the proportion of cotyledonary embryos.
- Taxon
- rapeseedt0
- Treatment
- growth substancee0
- Outcome
- proportion of cotyledonary embryos producede0
- Direction
- neither growth substance had any effecte0
Not reportedComparator
Verbatim
e0“neither growth substance had any effect on the proportion of cotyledonary embryos produced”
t0“rapeseed”
Claim · model-written summary
NAA–BA treatments showed no significant effect on root development after hormone-free regeneration.
- Taxon
- rapeseedt0
- Treatment
- NAA–BA treatmentse0
- Outcome
- root developmente0
- Direction
- no significant effectse0
Not reportedComparator
Verbatim
e0“no significant effects on either root development or shoot production attributable to the NAA–BA treatments could be detected”
t0“rapeseed”
Claim · model-written summary
NAA–BA treatments showed no significant effect on shoot production after hormone-free regeneration.
- Taxon
- rapeseedt0
- Treatment
- NAA–BA treatmentse0
- Outcome
- shoot productione0
- Direction
- no significant effectse0
Not reportedComparator
Verbatim
e0“no significant effects on either root development or shoot production attributable to the NAA–BA treatments could be detected”
t0“rapeseed”
Claim · model-written summary
Higher embryo yields were associated with slower development.
Verbatim
e0“higher embryo yields were associated with a slower rate of development”
t0“rapeseed”
Claim · model-written summary
Higher embryo yields were associated with greater morphological synchrony.
Verbatim
e0“higher embryo yields were associated with a slower rate of development, but a greater degree of morphological synchrony within individual cultures”
t0“rapeseed”
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.