Abstract
Sugarcane is cultivated in tropical and subtropical regions where cold stress is not very common, but lower yields and reduced industrial quality of the plants are observed when it occurs. In our efforts to enhance cold tolerance in sugarcane, the gene encoding the enzyme isopentenyltransferase (ipt) under control of the cold inducible gene promoter AtCOR15a was transferred via biolistic transformation into sugarcane (Saccharum spp.) cv. RB855536. Semiquantitative RT-PCR using GAPDH encoding glyceraldehyde-3-phosphate dehydrogenase as the normalizer gene showed the increased expression of the ipt gene under cold stress. The detached leaves of genetically modified plants subjected to low temperatures showed visible reduction of leaf senescence in comparison to non-transgenic control plants. Induced overexpression of ipt gene also enhanced cold tolerance of non-acclimated whole plants. After being subjected to freezing temperature, leaf total chlorophyll contents of transgenic plants were up to 31 % higher than in wild type plants. Also, lower malondialdehyde content and electrolyte leakage indicated less damage induced by cold in transgenic plants. Thus, the expression of ipt driven by the stress inducible COR15a promoter did not affect plant growth while providing a greater tolerance to cold stress.
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Claims 7
Claim · model-written summary
Cold stress increased ipt expression in transgenic sugarcane.
- Taxon
- sugarcanee1
- Material
- RB855536e1
- Treatment
- cold stresse0
- Gene
- ipte0
- Outcome
- expression of the ipt genee0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“increased expression of the ipt gene under cold stress”
e1“sugarcane (Saccharum spp.) cv. RB855536”
Claim · model-written summary
Transgenic detached leaves showed reduced senescence under low temperature compared with non-transgenic controls.
- Taxon
- sugarcanee1
- Organ / stage
- detached leavese0
- Treatment
- genetically modified plants subjected to low temperaturese0
- Outcome
- leaf senescencee0
- Direction
- reductione0
- Comparator
- non-transgenic control plantse0
Not reportedGene
Verbatim
e0“The detached leaves of genetically modified plants subjected to low temperatures showed visible reduction of leaf senescence in comparison to non-transgenic control plants”
e1“sugarcane (Saccharum spp.) cv. RB855536”
Claim · model-written summary
Induced ipt overexpression enhanced cold tolerance in non-acclimated whole plants.
- Taxon
- sugarcanee1
- Treatment
- Induced overexpression of ipt genee0
- Gene
- ipte0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
- Qualifier
- non-acclimated whole plantse0
Not reportedComparator
Verbatim
e0“Induced overexpression of ipt gene also enhanced cold tolerance of non-acclimated whole plants”
e1“sugarcane (Saccharum spp.) cv. RB855536”
Claim · model-written summary
After freezing, transgenic leaf chlorophyll content was up to 31% higher than in wild type.
Verbatim
e0“After being subjected to freezing temperature, leaf total chlorophyll contents of transgenic plants were up to 31 % higher than in wild type plants”
e1“sugarcane (Saccharum spp.) cv. RB855536”
Claim · model-written summary
Transgenic plants had lower malondialdehyde content after cold exposure.
- Taxon
- sugarcanee1
- Treatment
- transgenic plantse0
- Outcome
- malondialdehyde contente0
- Direction
- lowere0
- Qualifier
- colde0
Not reportedComparator
Verbatim
e0“lower malondialdehyde content and electrolyte leakage indicated less damage induced by cold in transgenic plants”
e1“sugarcane (Saccharum spp.) cv. RB855536”
Claim · model-written summary
Transgenic plants had lower electrolyte leakage after cold exposure.
- Taxon
- sugarcanee1
- Treatment
- transgenic plantse0
- Outcome
- electrolyte leakagee0
- Direction
- lowere0
- Qualifier
- colde0
Not reportedComparator
Verbatim
e0“lower malondialdehyde content and electrolyte leakage indicated less damage induced by cold in transgenic plants”
e1“sugarcane (Saccharum spp.) cv. RB855536”
Claim · model-written summary
Stress-inducible ipt expression did not affect plant growth.
- Taxon
- sugarcanee1
- Treatment
- expression of ipt driven by the stress inducible COR15a promotere0
- Gene
- ipte0
- Outcome
- plant growthe0
- Direction
- did not affecte0
Not reportedComparator
Verbatim
e0“expression of ipt driven by the stress inducible COR15a promoter did not affect plant growth”
e1“sugarcane (Saccharum spp.) cv. RB855536”
The same questions in other papers
transgenic · growth · Saccharum officinarum (sugarcane)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.