Abstract
Sugarcane is cultivated in tropical and subtropical regions where cold stress is not very common, but lower yields and reduced industrial quality of the plants are observed when it occurs. In our efforts to enhance cold tolerance in sugarcane, the gene encoding the enzyme isopentenyltransferase (ipt) under control of the cold inducible gene promoter AtCOR15a was transferred via biolistic transformation into sugarcane (Saccharum spp.) cv. RB855536. Semiquantitative RT-PCR using GAPDH encoding glyceraldehyde-3-phosphate dehydrogenase as the normalizer gene showed the increased expression of the ipt gene under cold stress. The detached leaves of genetically modified plants subjected to low temperatures showed visible reduction of leaf senescence in comparison to non-transgenic control plants. Induced overexpression of ipt gene also enhanced cold tolerance of non-acclimated whole plants. After being subjected to freezing temperature, leaf total chlorophyll contents of transgenic plants were up to 31 % higher than in wild type plants. Also, lower malondialdehyde content and electrolyte leakage indicated less damage induced by cold in transgenic plants. Thus, the expression of ipt driven by the stress inducible COR15a promoter did not affect plant growth while providing a greater tolerance to cold stress.
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Claims 8
Claim · model-written summary
Cold stress increased ipt expression in transformed sugarcane.
Verbatim
t0“sugarcane”
e0“increased expression of the ipt gene under cold stress”
Claim · model-written summary
Modified detached leaves had less senescence under low temperature than non-transgenic controls.
Verbatim
t0“sugarcane”
e0“The detached leaves of genetically modified plants subjected to low temperatures showed visible reduction of leaf senescence in comparison to non-transgenic control plants.”
Claim · model-written summary
Induced ipt overexpression enhanced cold tolerance in non-acclimated whole plants.
- Taxon
- sugarcanet0
- Treatment
- Induced overexpression of ipt genee0
- Gene
- ipt genee0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Not reportedComparator
Verbatim
t0“sugarcane”
e0“Induced overexpression of ipt gene also enhanced cold tolerance of non-acclimated whole plants.”
Claim · model-written summary
After freezing, transgenic leaves had up to 31 % more total chlorophyll than wild type leaves.
Verbatim
t0“sugarcane”
e0“After being subjected to freezing temperature, leaf total chlorophyll contents of transgenic plants were up to 31 % higher than in wild type plants.”
Claim · model-written summary
Transgenic plants had lower malondialdehyde content under cold.
Not reportedComparator
Verbatim
t0“sugarcane”
e0“lower malondialdehyde content and electrolyte leakage indicated less damage induced by cold in transgenic plants”
Claim · model-written summary
Transgenic plants had lower electrolyte leakage under cold.
Not reportedComparator
Verbatim
t0“sugarcane”
e0“lower malondialdehyde content and electrolyte leakage indicated less damage induced by cold in transgenic plants”
Claim · model-written summary
Stress-inducible ipt expression did not affect growth.
- Taxon
- sugarcanet0
- Treatment
- expression of ipt driven by the stress inducible COR15a promotere0
- Outcome
- plant growthe0
- Direction
- did not affecte0
Not reportedComparator
Verbatim
t0“sugarcane”
e0“the expression of ipt driven by the stress inducible COR15a promoter did not affect plant growth”
Claim · model-written summary
Stress-inducible ipt expression provided greater cold tolerance.
- Taxon
- sugarcanet0
- Treatment
- expression of ipt driven by the stress inducible COR15a promotere0
- Outcome
- tolerance to cold stresse0
- Direction
- greatere0
Not reportedComparator
Verbatim
t0“sugarcane”
e0“the expression of ipt driven by the stress inducible COR15a promoter did not affect plant growth while providing a greater tolerance to cold stress”
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