Paper · 2004 · Communications in Soil Science and Plant Analysis

Influence of Storage Methods on Total Nitrogen Analysis in Rice Leaves

Jianliang Huang; Shaobing Peng

4
claims
8
verbatim quotes
5
citations
1
research areas
—
Not open access

Abstract

Crop researchers often store ground plant tissue samples for considerable time before determining their nitrogen (N) concentration. The objective of this study was to determine the effects of storage methods and storage duration on total N analysis in rice leaves. The storage methods investigated were (1) keeping ground leaf tissue in a paper bag, (2) storage in a bottle with additional drying at 70°C for 24 h, or (3) in a bottle without additional drying. Paper bags and sealed bottles were kept under ambient condition. Leaf N concentration was measured about once a month for two years by micro-Kjeldahl method. Storage methods had little effect on leaf N concentration during the two-year storage period. The difference in leaf N concentration was 0.1–0.2 g kg−1 between the samples stored in paper bags and in bottles, which was less than 0.5% of mean value of leaf N concentration. Leaf N concentration was not affected over the two-year storage duration. These results suggest that it is sufficient to keep plant tissue samples in paper bags under ambient condition for at least two years for measurement of leaf N concentration.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 4

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Storage method had little effect on rice leaf nitrogen concentration over two years.

Treatment
Storage methodse0
Outcome
leaf N concentratione0
Direction
little effecte0
Qualifier
two-year storage periode0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Storage methods had little effect on leaf N concentration during the two-year storage period”

✓ located verbatim in the source · abstract, characters 619–711 · show in text ↑
e1

“rice leaves”

✓ located verbatim in the source · abstract, characters 252–263 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Rice leaf nitrogen concentration differed by 0.1–0.2 g kg−1 between paper bag and bottle storage.

Treatment
paper bagse0
Outcome
leaf N concentratione0
Direction
differencee0
Value
0.1–0.2 g kg−1e0
Qualifier
less than 0.5% of mean value of leaf N concentratione0
Comparator
bottlese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The difference in leaf N concentration was 0.1–0.2 g kg−1 between the samples stored in paper bags and in bottles, which was less than 0.5% of mean value of leaf N concentration”

✓ located verbatim in the source · abstract, characters 713–890 · show in text ↑
e1

“rice leaves”

✓ located verbatim in the source · abstract, characters 252–263 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Two-year storage duration did not affect rice leaf nitrogen concentration.

Treatment
two-year storage duratione0
Outcome
Leaf N concentratione0
Direction
not affectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Leaf N concentration was not affected over the two-year storage duration”

✓ located verbatim in the source · abstract, characters 892–964 · show in text ↑
e1

“rice leaves”

✓ located verbatim in the source · abstract, characters 252–263 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Ambient paper bag storage is sufficient for at least two years before measuring plant tissue nitrogen.

Outcome
keep plant tissue samples in paper bags under ambient condition for at least two years for measurement of leaf N concentratione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“it is sufficient to keep plant tissue samples in paper bags under ambient condition for at least two years for measurement of leaf N concentration”

✓ located verbatim in the source · abstract, characters 993–1139 · show in text ↑
e1

“rice leaves”

✓ located verbatim in the source · abstract, characters 252–263 · show in text ↑

storage · nutrient: content · Oryza sativa (rice)Positive and negative results

12 papers: 9 positive, 3 negative or no effect

2 papers in the same direction as this paper · 9 in the opposite direction

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.