Abstract
This study was conducted to examine protein synthesis and l-[(35)S] methionine incorporation into the endosperm of Zea mays L. kernels developing in vitro. Two-day-old kernels of the inbred line W64A were placed in culture on a defined medium containing 10 microCuries l-[(35)S] methionine per milliliter (13 milliCuries per millimole) and harvested at 10, 15, 20, 25, 30, 35, and 40 days after pollination. Cultured kernels attained a final endosperm mass of 120 milligrams compared to 175 milligrams for field-grown controls. Field and cultured kernels had similar concentrations (microgram per milligram endospern) for total protein, albumin plus globulin, zein, and glutelin fractions at most kernel ages.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing patterns for endosperm proteins were similar for field and cultured kernels throughout development. By 15 days, over 70% of the l-[(35)S]methionine taken up was present in endosperm proteins. Label incorporation visualized by fluorography generally followed the protein intensity of the stained gels. The high methionine content, low molecular weight zeins (i.e. 15 and 9 kilodaltons) were highly labeled. All of the radioactivity in hydrolyzed zein samples was recovered in the methionine peak indicating minimal conversion to l-[(35)S]cysteine. The procedure described here is suitable for long term culture and labeling experiments in which continued kernel development is required.
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Claims 8
Claim · model-written summary
Cultured endosperm reached 120 mg versus 175 mg in field controls.
- Treatment
- Cultured kernelse0
- Outcome
- final endosperm masse0
- Value
- 120 milligramse0
- Comparator
- field-grown controlse0
Zea maysNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize”
e0“Cultured kernels attained a final endosperm mass of 120 milligrams compared to 175 milligrams for field-grown controls”
e1“Zea mays L.”
Claim · model-written summary
Protein fractions had similar concentrations in field and cultured kernels.
- Outcome
- concentrations (microgram per milligram endospern) for total protein, albumin plus globulin, zein, and glutelin fractionse0
- Direction
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize”
e0“Field and cultured kernels had similar concentrations (microgram per milligram endospern) for total protein, albumin plus globulin, zein, and glutelin fractions at most kernel ages”
e1“Zea mays L.”
Claim · model-written summary
Endosperm protein electrophoresis patterns were similar throughout development.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize”
e0“patterns for endosperm proteins were similar for field and cultured kernels throughout development”
e1“Zea mays L.”
Claim · model-written summary
Over 70% of absorbed labeled methionine entered endosperm proteins by day 15.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize”
e0“By 15 days, over 70% of the l-[(35)S]methionine taken up was present in endosperm proteins”
e1“Zea mays L.”
Claim · model-written summary
Fluorographic label incorporation followed stained protein intensity.
- Outcome
- Label incorporation visualized by fluorographye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize”
e0“Label incorporation visualized by fluorography generally followed the protein intensity of the stained gels”
e1“Zea mays L.”
Claim · model-written summary
Low-molecular-weight high-methionine zeins were highly labeled.
- Outcome
- low molecular weight zeins (i.e. 15 and 9 kilodaltons) were highly labelede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize”
e0“The high methionine content, low molecular weight zeins (i.e. 15 and 9 kilodaltons) were highly labeled”
e1“Zea mays L.”
Claim · model-written summary
All hydrolyzed zein radioactivity was recovered in methionine, indicating little cysteine conversion.
- Outcome
- radioactivity in hydrolyzed zein samples was recovered in the methionine peake0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize”
e0“All of the radioactivity in hydrolyzed zein samples was recovered in the methionine peak indicating minimal conversion to l-[(35)S]cysteine”
e1“Zea mays L.”
Claim · model-written summary
The culture method supports long-term kernel development and labeling.
- Outcome
- suitable for long term culture and labeling experimentse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize”
e0“The procedure described here is suitable for long term culture and labeling experiments in which continued kernel development is required”
e1“Zea mays L.”