Abstract
BACKGROUND: Grafting is widely used in the agriculture of fruit-bearing crops; rootstocks are known to confer differences in scion biomass in addition to improving other traits of agricultural interest. However, little is known about the effect of rootstocks on scion gene expression. The objective of this study was to determine whether hetero-grafting the grapevine variety Vitis vinifera cv. 'Cabernet Sauvignon N' with two different rootstocks alters gene expression in the shoot apex in comparison to the auto-grafted control. Cabernet Sauvignon was hetero-grafted with two commercial rootstock genotypes and auto-grafted with itself. Vigor was quantified by measurements of root, stem, leaf and trunk biomass. Gene expression profiling was done using a whole genome grapevine microarray; four pools of five shoot apex samples were harvested 4 months after grafting for each scion/rootstock combination. RESULTS: The rootstocks increased stem biomass or conferred increased vigor by the end of the first growth cycle. Globally hetero-grafting two different genotypes together triggered an increase in shoot apex gene expression; however no genes were differentially expressed between the two hetero-grafts. The functional categories related to DNA, chromatin structure, histones, flavonoids and leucine rich repeat containing receptor kinases were the most enriched in the up-regulated genes in the shoot apex of hetero-grafted plants. CONCLUSIONS: The choice of rootstock genotype had little effect on the gene expression in the shoot apex; this could suggest that auto- and hetero-grafting was the major factor regulating gene expression.
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Claims 5
Claim · model-written summary
Rootstocks increased grapevine scion stem biomass relative to the auto-grafted control.
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“shoot apical meristem of grapevine”
e1“The rootstocks increased stem biomass”
e2“in comparison to the auto-grafted control”
Claim · model-written summary
Hetero-grafting increased shoot apex gene expression compared with the auto-grafted control.
- Organ / stage
- shoot apexe3
- Treatment
- hetero-grafting two different genotypes togethere4
- Outcome
- shoot apex gene expressione3
- Direction
- increasee3
- Comparator
- auto-grafted controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“shoot apical meristem of grapevine”
e2“in comparison to the auto-grafted control”
e3“triggered an increase in shoot apex gene expression”
e4“Globally hetero-grafting two different genotypes together”
Claim · model-written summary
No genes were differentially expressed between the two hetero-grafts with different rootstocks.
- Treatment
- with two different rootstockse6
- Outcome
- differentially expressede5
- Direction
- no_changeinferrede5
- Comparator
- the two hetero-graftse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“shoot apical meristem of grapevine”
e5“however no genes were differentially expressed between the two hetero-grafts”
e6“with two different rootstocks”
Claim · model-written summary
Shoot apex up-regulated genes of hetero-grafted grapevine were most enriched in DNA, chromatin, histone, flavonoid and receptor kinase categories.
- Organ / stage
- shoot apexe7
- Outcome
- the most enriched in the up-regulated genese7
- Value
- DNA, chromatin structure, histones, flavonoids and leucine rich repeat containing receptor kinasese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“shoot apical meristem of grapevine”
e7“The functional categories related to DNA, chromatin structure, histones, flavonoids and leucine rich repeat containing receptor kinases were the most enriched in the up-regulated genes in the shoot apex of hetero-grafted plants”
Claim · model-written summary
Auto- and hetero-grafting may be the major factor regulating shoot apex gene expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“shoot apical meristem of grapevine”
e2“auto- and hetero-grafting was the major factor regulating gene expression”
The same questions in other papers
grafting · growth · VitisPositive and negative results
19 papers in the same direction as this paper · 10 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- no effect
- no effect
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.