Abstract
In vitro culture of soybean (Glycine max [L.] Merr.) seeds and zygotic embryos is used to study factors affecting seed growth, maturation, desiccation tolerance, and germination. While germination of in vitro matured zygotic embryos is usually greater than 80%, seedling growth is often lower than that of seeds matured in planta. Soluble carbohydrates in axis and cotyledon tissues of soybean zygotic embryos at 0–32 days of in vitro culture were assayed before drying, after fast drying, and after slow drying and related to changes in germination and axis growth of seedlings. Germinability was improved by slow drying. A low mass ratio of sucrose to raffinose plus stachyose was associated with germinability but was not sufficient for the maintenance of high seedling growth after prolonged culture in vitro. The loss of seedling growth as a function of days in culture correlated (r2=0.94) with a decline in stachyose concentration in axis tissues after slow drying. Total pinitol did not exceed that present in embryos before culture, and accumulation of galactopinitols and fagopyritol B1 was limited in embryos grown in vitro. Responses of zygotic embryos to in vitro culture may be useful in understanding problems associated with the conversion of soybean somatic embryos to plantlets and vigorous growth of plantlets.
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Claims 6
Claim · model-written summary
Slow drying improved germinability of soybean zygotic embryos compared with fast drying.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- zygotic embryost0
- Treatment
- slow dryinge1
- Outcome
- Germinabilitye1
- Direction
- improvede1
- Comparator
- fast dryinge2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max L. Merrill) zygotic embryos during in vitro maturation”
e1“Germinability was improved by slow drying”
e2“before drying, after fast drying, and after slow drying”
Claim · model-written summary
A low sucrose to raffinose plus stachyose ratio was associated with higher germinability.
- Taxon
- soybeanGlycine maxt0
- Compound
- sucrose to raffinose plus stachyosee3
- Outcome
- germinabilitye3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max L. Merrill) zygotic embryos during in vitro maturation”
e3“A low mass ratio of sucrose to raffinose plus stachyose was associated with germinability but was not sufficient for the maintenance of high seedling growth after prolonged culture in vitro”
Claim · model-written summary
A low sucrose to raffinose plus stachyose ratio was not sufficient to maintain high seedling growth after prolonged in vitro culture.
- Taxon
- soybeanGlycine maxt0
- Compound
- sucrose to raffinose plus stachyosee3
- Outcome
- maintenance of high seedling growthe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max L. Merrill) zygotic embryos during in vitro maturation”
e3“A low mass ratio of sucrose to raffinose plus stachyose was associated with germinability but was not sufficient for the maintenance of high seedling growth after prolonged culture in vitro”
Claim · model-written summary
Loss of seedling growth correlated with a decline in stachyose concentration in axis tissues after slow drying (r2=0.94).
- Taxon
- soybeanGlycine maxt0
- Compound
- stachyosee4
- Outcome
- stachyose concentration in axis tissuese4
- Direction
- declinee4
- Value
- r2=0.94e4
- Qualifier
- loss of seedling growthe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max L. Merrill) zygotic embryos during in vitro maturation”
e4“The loss of seedling growth as a function of days in culture correlated (r2=0.94) with a decline in stachyose concentration in axis tissues after slow drying”
Claim · model-written summary
Total pinitol in soybean zygotic embryos did not exceed the level present before in vitro culture.
- Taxon
- soybeanGlycine maxt0
- Outcome
- Total pinitole5
- Qualifier
- did not exceed that present in embryos before culturee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max L. Merrill) zygotic embryos during in vitro maturation”
e5“Total pinitol did not exceed that present in embryos before culture”
Claim · model-written summary
Accumulation of galactopinitols and fagopyritol B1 was limited in soybean embryos grown in vitro.
- Taxon
- soybeanGlycine maxt0
- Outcome
- accumulation of galactopinitols and fagopyritol B1e6
- Direction
- limitede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean (Glycine max L. Merrill) zygotic embryos during in vitro maturation”
e6“accumulation of galactopinitols and fagopyritol B1 was limited in embryos grown in vitro”
The same questions in other papers
processing · growth · Glycine max (soybean)Positive and negative results
29 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- no effect
39 papers more in this group
See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.