Abstract
Isoflavones have been reported to have putative health-beneficial properties, which has led to increased interest and demand for soybeans and soy-based products. This study was conducted to determine the effects of high-temperature stress on isoflavone concentration and expression of four key genes involved in isoflavone synthesis (i.e., CHS7, CHS8, IFS1, and IFS2) in both soybean pods and seeds during their late reproductive stage (i.e., R5-R8). Isoflavone concentrations were quantified using high-performance liquid chromatography (HPLC), and gene expression was studied using quantitative real-time (qRT)-PCR. High-temperature stress [33/25 °C (day/night temperatures)] imposed at the late reproductive stage (R5-R8) reduced total isoflavone concentration by 46-86 and 20-73% in seeds and pods, respectively, the reduction depending on the stage of maturity. At stage R5, the reduction in total isoflavone concentration was greater in seeds than in pods, whereas at subsequent stages, the reverse was observed. High-temperature stress had a large impact on the expression of CHS7, CHS8, IFS1, and IFS2 in both seeds and pods. In seeds, temperature stress reduced the expression of one gene at the R5 stage (CHS8), two genes at the R6 stage (CHS7 and IFS1), and all four genes at the R7 stage, the reduction ranging between 35 and 97%. In pods, high-temperature stress affected the expression of two genes at the R6 stage (CHS7 and IFS2) and all four genes at the R7 stage. Unlike in seeds, at the R6 stage, high temperature increased the expression of CHS7 and IFS2 by 72 and 736%, respectively, whereas at R7 stage the expression of all genes was reduced by an average of 97%. The present study reveals that high-temperature stress initiated at the R5 stage and maintained until maturation (i.e., R8 stage) has a rapid and sustained negative effect on isoflavone concentration in both seeds and pods. High temperature also affects gene expression; however, there was no clear correlation between isoflavone concentration and gene expression.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Heat stress reduced total isoflavones in soybean seeds by 46-86%.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- seedse0
- Treatment
- High-temperature stress [33/25 °C (day/night temperatures)]e0
- Outcome
- total isoflavone concentratione0
- Direction
- reducede0
- Value
- 46-86e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High-temperature stress [33/25 °C (day/night temperatures)] imposed at the late reproductive stage (R5-R8) reduced total isoflavone concentration by 46-86 and 20-73% in seeds and pods, respectively”
t0“Soybean”
Claim · model-written summary
Heat stress reduced total isoflavones in soybean pods by 20-73%.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- podse0
- Treatment
- High-temperature stress [33/25 °C (day/night temperatures)]e0
- Outcome
- total isoflavone concentratione0
- Direction
- reducede0
- Value
- 20-73%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High-temperature stress [33/25 °C (day/night temperatures)] imposed at the late reproductive stage (R5-R8) reduced total isoflavone concentration by 46-86 and 20-73% in seeds and pods, respectively”
t0“Soybean”
Claim · model-written summary
At R5, the heat-related isoflavone reduction was greater in seeds than pods.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- seedse0
- Outcome
- reduction in total isoflavone concentratione0
- Direction
- greatere0
- Comparator
- podse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At stage R5, the reduction in total isoflavone concentration was greater in seeds than in pods”
t0“Soybean”
Claim · model-written summary
At later stages, the heat-related isoflavone reduction was greater in pods than seeds.
- Taxon
- soybeanGlycine maxt0
- Outcome
- reversee0
- Direction
- reversee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas at subsequent stages, the reverse was observed.”
t0“Soybean”
Claim · model-written summary
Heat reduced CHS8 expression in seeds at R5.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- seedse0
- Treatment
- temperature stresse0
- Gene
- CHS8e0
- Outcome
- expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In seeds, temperature stress reduced the expression of one gene at the R5 stage (CHS8)”
t0“Soybean”
Claim · model-written summary
Heat reduced CHS7 and IFS1 expression in seeds at R6.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- seedse0
- Treatment
- temperature stresse0
- Gene
- CHS7 and IFS1e0
- Outcome
- expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In seeds, temperature stress reduced the expression of one gene at the R5 stage (CHS8), two genes at the R6 stage (CHS7 and IFS1)”
t0“Soybean”
Claim · model-written summary
Heat reduced all four assayed gene expressions in seeds at R7 by 35 to 97%.
- Taxon
- soybeanGlycine maxt0
- Outcome
- all four genese0
- Direction
- reductione0
- Value
- 35 and 97%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and all four genes at the R7 stage, the reduction ranging between 35 and 97%”
t0“Soybean”
Claim · model-written summary
Heat raised CHS7 expression in pods at R6 by 72%.
- Taxon
- soybeanGlycine maxt0
- Treatment
- high temperaturee0
- Gene
- CHS7e0
- Outcome
- expressione0
- Direction
- increasede0
- Value
- 72e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at the R6 stage, high temperature increased the expression of CHS7 and IFS2 by 72 and 736%, respectively”
t0“Soybean”
Claim · model-written summary
Heat raised IFS2 expression in pods at R6 by 736%.
- Taxon
- soybeanGlycine maxt0
- Treatment
- high temperaturee0
- Gene
- IFS2e0
- Outcome
- expressione0
- Direction
- increasede0
- Value
- 736%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at the R6 stage, high temperature increased the expression of CHS7 and IFS2 by 72 and 736%, respectively”
t0“Soybean”
Claim · model-written summary
Heat reduced expression of all assayed genes in pods at R7 by an average of 97%.
- Taxon
- soybeanGlycine maxt0
- Outcome
- expression of all genese0
- Direction
- reducede0
- Value
- 97%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas at R7 stage the expression of all genes was reduced by an average of 97%.”
t0“Soybean”
Claim · model-written summary
Isoflavone concentration and gene expression showed no clear correlation.
- Taxon
- soybeanGlycine maxt0
- Gene
- gene expressione0
- Outcome
- isoflavone concentratione0
- Direction
- no clear correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no clear correlation between isoflavone concentration and gene expression.”
t0“Soybean”
The same questions in other papers
heat · molecular: metabolites · Glycine max (soybean)Positive and negative results
7 papers in the same direction as this paper · 14 in the opposite direction
- positive
- positive
- positive
18 papers more in this group
See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.