Abstract
Fungal pathogens usually have multiple genes that encode extracellular hydrolytic enzymes that may degrade the physical barriers in their hosts during the invasion process. Nectria hematococca, a plant pathogen, has two inducible pectate lyase (PL) genes (pel) encoding PL that can help degrade the carbohydrate barrier in the host. pelA is induced by pectin, whereas pelD is induced only in planta. We show that the disruption of either the pelA or pelD genes alone causes no detectable decrease in virulence. Disruption of both pelA and pelD drastically reduces virulence. Complementation of the double disruptant with pelD gene, or supplementation of the infection droplets of the double disruptant with either purified enzyme, PLA, or PLD, caused a recovery in virulence. These results show that PL is a virulence factor. Thus, we demonstrate that disruption of all functionally redundant genes is required to demonstrate the role of host barrier-degrading enzymes in pathogenesis and that dismissal of the role of such enzymes based on the effects of single-gene disruption may be premature.
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Claims 5
Claim · model-written summary
Disrupting either pelA or pelD alone caused no detectable decrease in virulence.
- Taxon
- Pisum sativum
Lathyrus oleraceust0 - Treatment
- disruption of either the pelA or pelD genes alonee0
- Gene
- pelAe0
- Outcome
- virulencee0
- Direction
- no detectable decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the disruption of either the pelA or pelD genes alone causes no detectable decrease in virulence”
t0“Pisum sativum”
Claim · model-written summary
Disrupting both pelA and pelD drastically reduced virulence.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- Disruption of both pelA and pelDe0
- Gene
- pelA and pelDe0
- Outcome
- virulencee0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of both pelA and pelD drastically reduces virulence”
t0“Pisum sativum”
Claim · model-written summary
Complementing the double disruptant with pelD restored virulence.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- Complementation of the double disruptant with pelD genee0
- Gene
- pelDe0
- Outcome
- virulencee0
- Direction
- recoverye0
- Comparator
- double disruptante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Complementation of the double disruptant with pelD gene, or supplementation of the infection droplets of the double disruptant with either purified enzyme, PLA, or PLD, caused a recovery in virulence”
t0“Pisum sativum”
Claim · model-written summary
Supplementing double disruptant infection droplets with PLA restored virulence.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- supplementation of the infection droplets of the double disruptant with either purified enzyme, PLAe0
- Gene
- PLAe0
- Outcome
- virulencee0
- Direction
- recoverye0
- Comparator
- double disruptante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“supplementation of the infection droplets of the double disruptant with either purified enzyme, PLA, or PLD, caused a recovery in virulence”
t0“Pisum sativum”
Claim · model-written summary
Supplementing double disruptant infection droplets with PLD restored virulence.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- PLDe0
- Gene
- PLDe0
- Outcome
- virulencee0
- Direction
- recoverye0
- Comparator
- double disruptante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“supplementation of the infection droplets of the double disruptant with either purified enzyme, PLA, or PLD, caused a recovery in virulence”
t0“Pisum sativum”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.