Abstract
Pisum sativum plant introduction (PI) line 269818 is resistant to potyvirus pea seedborne mosaic virus (PSbMV) isolates, categorized as pathotype P1, and is susceptible to pathotype P4 isolates. This difference in infectivity is determined by the viral genome-linked protein (VPg) cistron. Mutational analysis of VPg of PSbMV isolates DPD1 and NY representing pathotypes P1 and P4 revealed that codon changes affecting amino acids 105 to 117 in the central region of VPg influenced virulence on PI 269818. In contrast, infectivity on pea cultivar Dark Skinned Perfection, which is susceptible to both pathotypes, was not affected by the mutations. Mutants overcoming resistance in PI 269818 were analyzed for changes in the VPg coding region upon passage through PI 269818 and Dark Skinned Perfection. Adaptive changes were observed only upon passage through PI 269818 and only at codons from amino acid 105 to 117. Expression of DPD1 VPg in PI 269818 did not affect infection by NY, which suggests that VPg from DPD1 is not an elicitor of a general resistance response. The results are compatible with the hypothesis that viral amplification depends upon the interaction between VPg and a host factor.
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Claims 6
Claim · model-written summary
PI 269818 resisted P1 isolates and was susceptible to P4 isolates.
- Taxon
- Pisum sativum
Lathyrus oleraceuse0 - Material
- PI 269818e1
- Interactor
- pea seedborne mosaic viruse0pathogen
- Outcome
- resistant to potyvirus pea seedborne mosaic virus (PSbMV) isolates, categorized as pathotype P1, and is susceptible to pathotype P4 isolatese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pisum sativum plant introduction (PI) line 269818 is resistant to potyvirus pea seedborne mosaic virus (PSbMV) isolates, categorized as pathotype P1, and is susceptible to pathotype P4 isolates.”
e1“codon changes affecting amino acids 105 to 117 in the central region of VPg influenced virulence on PI 269818”
Claim · model-written summary
The viral VPg cistron determined the infectivity difference between P1 and P4 isolates on PI 269818.
- Taxon
- Pisum sativumLathyrus oleraceuse1
- Material
- PI 269818e2
- Interactor
- virale0pathogen
- Gene
- viral genome-linked protein (VPg) cistrone0
- Outcome
- difference in infectivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This difference in infectivity is determined by the viral genome-linked protein (VPg) cistron.”
e1“Pisum sativum plant introduction (PI) line 269818”
e2“codon changes affecting amino acids 105 to 117 in the central region of VPg influenced virulence on PI 269818”
Claim · model-written summary
VPg codon changes affecting amino acids 105 to 117 influenced virulence on PI 269818.
- Taxon
- Pisum sativumLathyrus oleraceuse1
- Material
- PI 269818e0
- Interactor
- PSbMVPea seed-borne mosaic viruse2pathogen
- Gene
- VPge0
- Outcome
- virulence on PI 269818e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“codon changes affecting amino acids 105 to 117 in the central region of VPg influenced virulence on PI 269818”
e1“Pisum sativum plant introduction (PI) line 269818”
e2“Pisum sativum plant introduction (PI) line 269818 is resistant to potyvirus pea seedborne mosaic virus (PSbMV) isolates, categorized as pathotype P1, and is susceptible to pathotype P4 isolates.”
Claim · model-written summary
The VPg mutations did not affect infectivity on Dark Skinned Perfection.
- Taxon
- Pisum sativumLathyrus oleraceuse1
- Material
- Dark Skinned Perfectione0
- Treatment
- mutationse0
- Outcome
- infectivitye0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infectivity on pea cultivar Dark Skinned Perfection, which is susceptible to both pathotypes, was not affected by the mutations”
e1“Pisum sativum plant introduction (PI) line 269818”
Claim · model-written summary
Adaptive VPg changes occurred only after passage through PI 269818 and only at codons for amino acids 105 to 117.
- Taxon
- Pisum sativumLathyrus oleraceuse1
- Material
- PI 269818e0
- Gene
- VPge2
- Outcome
- Adaptive changese0
- Qualifier
- only at codons from amino acid 105 to 117e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adaptive changes were observed only upon passage through PI 269818 and only at codons from amino acid 105 to 117.”
e1“Pisum sativum plant introduction (PI) line 269818”
e2“This difference in infectivity is determined by the viral genome-linked protein (VPg) cistron.”
Claim · model-written summary
Expression of DPD1 VPg in PI 269818 did not affect infection by NY.
- Taxon
- Pisum sativumLathyrus oleraceuse1
- Material
- PI 269818e0
- Treatment
- Expression of DPD1 VPge0
- Interactor
- NYe0pathogen
- Gene
- DPD1 VPge0
- Outcome
- infection by NYe0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of DPD1 VPg in PI 269818 did not affect infection by NY”
e1“Pisum sativum plant introduction (PI) line 269818”
The same questions in other papers
loss of function · disease · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.