Abstract
Recently we reported on a plasma membrane tobacco protein (designated NtCBP4) that binds calmodulin. When overexpressed in transgenic plants, NtCBP4 confers Pb2+ hypersensitivity associated with enhanced accumulation of this toxic metal. To further investigate possible modulation of Pb2+ tolerance in plants, we prepared transgenic plants that express a truncated version of this protein (designated NtCBP4DeltaC) from which its C-terminal, with the calmodulin-binding domain and part of the putative cyclic nucleotide-binding domain, was removed. In contrast to the phenotype of transgenic plants expressing the full-length gene, transgenic plants expressing the truncated gene showed improved tolerance to Pb2+, in addition to attenuated accumulation of this metal. Furthermore, disruption by T-DNA insertion mutagenesis of the Arabidopsis CNGC1 gene, which encodes a homologous protein, also conferred Pb2+ tolerance. We suggest that NtCBP4 and AtCNGC1 are components of a transport pathway responsible for Pb2+ entry into plant cells.
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Claims 4
Claim · model-written summary
Transgenic tobacco expressing truncated NtCBP4DeltaC showed improved Pb2+ tolerance compared with plants expressing the full-length gene.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- expressing the truncated genee1
- Gene
- NtCBP4DeltaCe5
- Outcome
- tolerance to Pb2+e1
- Direction
- improvede1
- Comparator
- transgenic plants expressing the full-length genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recently we reported on a plasma membrane tobacco protein (designated NtCBP4) that binds calmodulin”
e1“In contrast to the phenotype of transgenic plants expressing the full-length gene, transgenic plants expressing the truncated gene showed improved tolerance to Pb2+”
e5“we prepared transgenic plants that express a truncated version of this protein (designated NtCBP4DeltaC)”
Claim · model-written summary
Transgenic tobacco expressing truncated NtCBP4DeltaC attenuated accumulation of Pb2+ compared with plants expressing the full-length gene.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- expressing the truncated genee1
- Outcome
- accumulation of this metale2
- Direction
- attenuatede2
- Comparator
- transgenic plants expressing the full-length genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recently we reported on a plasma membrane tobacco protein (designated NtCBP4) that binds calmodulin”
e1“In contrast to the phenotype of transgenic plants expressing the full-length gene, transgenic plants expressing the truncated gene showed improved tolerance to Pb2+”
e2“in addition to attenuated accumulation of this metal”
Claim · model-written summary
Disruption of the Arabidopsis CNGC1 gene by T-DNA insertion mutagenesis conferred Pb2+ tolerance.
- Taxon
- Arabidopsis
Arabidopsise3 - Treatment
- disruption by T-DNA insertion mutagenesise3
- Gene
- Arabidopsis CNGC1 genee3
- Outcome
- Pb2+ tolerancee3
- Direction
- conferrede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Furthermore, disruption by T-DNA insertion mutagenesis of the Arabidopsis CNGC1 gene, which encodes a homologous protein, also conferred Pb2+ tolerance”
Claim · model-written summary
NtCBP4 and AtCNGC1 are suggested to be components of a transport pathway responsible for Pb2+ entry into plant cells.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- NtCBP4 and AtCNGC1e4
- Outcome
- transport pathway responsible for Pb2+ entrye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recently we reported on a plasma membrane tobacco protein (designated NtCBP4) that binds calmodulin”
e4“We suggest that NtCBP4 and AtCNGC1 are components of a transport pathway responsible for Pb2+ entry into plant cells”
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