Abstract
The disappearance of indole-3-acetic acid (IAA) from cell-free liquid culture medium was followed in response to nutrient salts found in Murashige-Skoog salt base, light, and pH range of 4 to 7. The loss of IAA was accelerated by light or Murashige-Skoog salts. However, the combination of both light and Murashige-Skoog salts acted synergistically to catalyze the destruction of over 80% of the original IAA within 7 days of continuous incubation. Under these same conditions, the loss of IAA was decreased to approximately 50% by adjusting the initial pH of the medium to 7. Iron was identified as the single major contributor to light-catalyzed destruction of IAA. Removal of nitrates, which represented 87% of the molar salt composition, also reduced the light-catalyzed loss of IAA. Treatments that protected IAA from degradation, such as darkness or removal of iron from the medium, suppressed the growth of muskmelon (Cucumis melo. Naud., var. reticulatus) callus tissue cultured for 30 days. Treatments in the light that rapidly degraded IAA resulted in maximum growth. Consequently, the brief exposure to IAA prior to degradation was apparently sufficient to initiate physiological changes required for growth. Possible approaches to the preservation of IAA during incubation are discussed.
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Claims 10
Claim · model-written summary
Light accelerated IAA loss in cell-free medium.
Not reportedTaxonComparator
Verbatim
e0“The loss of IAA was accelerated by light or Murashige-Skoog salts.”
Claim · model-written summary
Murashige-Skoog salts accelerated IAA loss in cell-free medium.
Not reportedTaxonComparator
Verbatim
e0“The loss of IAA was accelerated by light or Murashige-Skoog salts.”
Claim · model-written summary
Light and salts synergistically destroyed over 80% of IAA within seven days.
- Treatment
- both light and Murashige-Skoog saltse0
- Outcome
- destruction of over 80% of the original IAAe0
- Value
- over 80%e0
- Qualifier
- within 7 days of continuous incubatione0
Not reportedTaxonComparatorDirection
Verbatim
e0“the combination of both light and Murashige-Skoog salts acted synergistically to catalyze the destruction of over 80% of the original IAA within 7 days of continuous incubation”
Claim · model-written summary
Initial pH 7 reduced IAA loss to about 50%.
- Treatment
- adjusting the initial pH of the medium to 7e0
- Outcome
- loss of IAAe0
- Direction
- decreasede0
- Value
- approximately 50%e0
Not reportedTaxonComparator
Verbatim
e0“the loss of IAA was decreased to approximately 50% by adjusting the initial pH of the medium to 7”
Claim · model-written summary
Iron was the major contributor to light-driven IAA destruction.
Not reportedTaxon
Verbatim
e0“Iron was identified as the single major contributor to light-catalyzed destruction of IAA.”
Claim · model-written summary
Nitrate removal reduced light-driven IAA loss.
Not reportedTaxonComparator
Verbatim
e0“Removal of nitrates, which represented 87% of the molar salt composition, also reduced the light-catalyzed loss of IAA.”
Claim · model-written summary
Darkness suppressed muskmelon callus growth.
- Taxon
- muskmelon
.jpg?width=60)
Cucumis meloe0 - Treatment
- darknesse0
- Outcome
- growth of muskmelon (Cucumis melo. Naud., var. reticulatus) callus tissuee0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Treatments that protected IAA from degradation, such as darkness or removal of iron from the medium, suppressed the growth of muskmelon (Cucumis melo. Naud., var. reticulatus) callus tissue cultured for 30 days.”
Claim · model-written summary
Removing iron suppressed muskmelon callus growth.
- Taxon
- muskmelonCucumis meloe0
- Treatment
- removal of iron from the mediume0
- Outcome
- growth of muskmelon (Cucumis melo. Naud., var. reticulatus) callus tissuee0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatments that protected IAA from degradation, such as darkness or removal of iron from the medium, suppressed the growth of muskmelon (Cucumis melo. Naud., var. reticulatus) callus tissue cultured for 30 days.”
Claim · model-written summary
Light treatments that rapidly degraded IAA gave maximum callus growth.
- Taxon
- muskmelonCucumis melot0
- Treatment
- Treatments in the lighte0
- Outcome
- growthe0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“muskmelon”
e0“Treatments in the light that rapidly degraded IAA resulted in maximum growth.”
Claim · model-written summary
Brief IAA exposure appeared sufficient to initiate changes needed for growth.
- Taxon
- muskmelonCucumis melot0
- Treatment
- brief exposure to IAAe0
- Outcome
- physiological changes required for growthe0
- Qualifier
- apparently sufficiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“muskmelon”
e0“the brief exposure to IAA prior to degradation was apparently sufficient to initiate physiological changes required for growth.”
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iron atom · growth · Cucumis melo (muskmelon)Results differ
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light · growth · Cucumis melo (muskmelon)Positive and negative results
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See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.