Abstract
BACKGROUND: Plants have evolved an array of constitutive and inducible defense strategies to restrict pathogen ingress. However, some pathogens still manage to invade plants and impair growth and productivity. Previous studies have revealed several key regulators of defense responses, and efforts have been made to use this information to develop disease resistant crop plants. These efforts are often hampered by the complexity of defense signaling pathways. To further elucidate the complexity of defense responses, we screened a population of T-DNA mutants in Colombia-0 background that displayed altered defense responses to virulent Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). RESULTS: In this study, we demonstrated that the Arabidopsis Purple Acid Phosphatse5 (PAP5) gene, induced under prolonged phosphate (Pi) starvation, is required for maintaining basal resistance to certain pathogens. The expression of PAP5 was distinctly induced only under prolonged Pi starvation and during the early stage of Pst DC3000 infection (6 h.p.i). T-DNA tagged mutant pap5 displayed enhanced susceptibility to the virulent bacterial pathogen Pst DC3000. The pap5 mutation greatly reduced the expression of pathogen inducible gene PR1 compared to wild-type plants. Similarly, other defense related genes including ICS1 and PDF1.2 were impaired in pap5 plants. Moreover, application of BTH (an analog of SA) restored PR1 expression in pap5 plants. CONCLUSION: Taken together, our results demonstrate the requirement of PAP5 for maintaining basal resistance against Pst DC3000. Furthermore, our results provide evidence that PAP5 acts upstream of SA accumulation to regulate the expression of other defense responsive genes. We also provide the first experimental evidence indicating the role PAP5 in plant defense responses.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
PAP5 expression was induced only under prolonged phosphate starvation and early Pst DC3000 infection.
- Taxon
- Arabidopsis
Arabidopsist0 - Interactor
- Pst DC3000e0pathogen
- Gene
- PAP5e0
- Outcome
- expression of PAP5e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of PAP5 was distinctly induced only under prolonged Pi starvation and during the early stage of Pst DC3000 infection (6 h.p.i)”
t0“Arabidopsis”
Claim · model-written summary
pap5 mutants showed enhanced susceptibility to Pst DC3000.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pap5e0
- Interactor
- Pst DC3000e0pathogen
- Outcome
- susceptibilitye0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“T-DNA tagged mutant pap5 displayed enhanced susceptibility to the virulent bacterial pathogen Pst DC3000”
t0“Arabidopsis”
Claim · model-written summary
pap5 mutation reduced pathogen-inducible PR1 expression compared with wild type.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pap5e0
- Gene
- PR1e0
- Outcome
- expression of pathogen inducible gene PR1e0
- Direction
- reducede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pap5 mutation greatly reduced the expression of pathogen inducible gene PR1 compared to wild-type plants”
t0“Arabidopsis”
Claim · model-written summary
ICS1 and PDF1.2 expression was impaired in pap5 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pap5e0
- Gene
- ICS1 and PDF1.2e0
- Outcome
- defense related genes including ICS1 and PDF1.2e0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“other defense related genes including ICS1 and PDF1.2 were impaired in pap5 plants”
t0“Arabidopsis”
Claim · model-written summary
BTH application restored PR1 expression in pap5 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pap5e0
- Treatment
- application of BTHe0
- Gene
- PR1e0
- Outcome
- PR1 expressione0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of BTH (an analog of SA) restored PR1 expression in pap5 plants”
t0“Arabidopsis”
Claim · model-written summary
Authors infer PAP5 acts upstream of SA accumulation to regulate defense-responsive genes.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PAP5e0
- Compound
- SAe0
- Outcome
- expression of other defense responsive genese0
- Qualifier
- upstream of SA accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PAP5 acts upstream of SA accumulation to regulate the expression of other defense responsive genes”
t0“Arabidopsis”
The same questions in other papers
acibenzolar-S-methyl · molecular: expression · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.