Abstract
summary A method is described of measuring dye diffusivity through the cell‐wall apoplast of roots, using sections of fresh material cut by hand under paraffin oil. Root pieces of Vicia faba and Glycine max were immersed in a solution of sulphorhodamine G for short periods, and the advance of the dye through the cell‐wall apoplast was measured, either in a number of sections cut after various periods of diffusion, or by photographing the same section at intervals while diffusion into it from the surface was observed under the microscope. Fick's second law was used to estimate the diffusivity. Diffusivities, expressed relative to the diffusivity in water (D aq ) were in the range D aq /16 to D aq /55 for Vicia roots. The values for Glycine roots showed a wide scatter, with a modal value of D aq /1700. Comparison of this rapid method with the much more laborious stabilization of the dye by freeze‐substitution confirmed the values of measured diffusivities in Vicia.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 3
Claim · model-written summary
Relative dye diffusivity in Vicia roots ranged from D aq /16 to D aq /55.
- Organ / stage
- rootse0
- Outcome
- Diffusivitiese0
- Value
- D aq /16 to D aq /55e0
- Comparator
- diffusivity in watere0
ViciaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Diffusivities, expressed relative to the diffusivity in water (D aq ) were in the range D aq /16 to D aq /55 for Vicia roots.”
Claim · model-written summary
Glycine roots had scattered diffusivities with a modal value of D aq /1700.
GlycineOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The values for Glycine roots showed a wide scatter, with a modal value of D aq /1700.”
Claim · model-written summary
Freeze-substitution comparison confirmed diffusivities measured by the rapid method in Vicia.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparison of this rapid method with the much more laborious stabilization of the dye by freeze‐substitution confirmed the values of measured diffusivities in Vicia.”