Abstract
Broad-spectrum disease resistance is a highly valuable trait in plant breeding and attracts special attention in research. The Arabidopsis gene locus RESISTANCE TO POWDERY MILDEW 8 (RPW8) contains two adjacent homologous genes, RPW8.1 and RPW8.2, and confers broad-spectrum resistance to powdery mildew. Remarkably, the RPW8.2 protein is specifically localized to the extrahaustorial membrane (EHM) encasing the feeding structure of powdery mildew whereby RPW8.2 activates haustorium-targeted defenses. Here, we show that ectopic expression of the yellow fluorescent protein (YFP)-tagged RPW8.1 from the native promoter leads to unique cell death lesions and enhances resistance to virulent fungal and oomycete pathogens that cause powdery mildew and downy mildew diseases, respectively. In powdery mildew-infected plants, RPW8.1-YFP accumulates at higher levels in the mesophyll cells underneath the infected epidermal cells where RPW8.2-YFP is mainly expressed. This cell type-preferential protein accumulation pattern largely correlates with that of H(2)O(2) accumulation, suggesting that RPW8.1 may spatially collaborate with RPW8.2 in activation of resistance to powdery mildew. Interestingly, when ectopically expressed from the RPW8.2 promoter, RPW8.1-YFP is also targeted to the EHM of powdery mildew and the transgenic plants display resistance to both powdery mildew and downy mildew. Using YFP as a reporter, we further reveal that the RPW8.1 promoter is constitutively active but induced to higher levels in cells at the infection site, whereas the RPW8.2 promoter is activated specifically in cells at the infection site. Taken together, our results suggest that RPW8.1 (and its promoter) is functionally distinct from RPW8.2 and may have a higher potential in engineering broad-spectrum resistance in plants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
RPW8.1 ectopic expression caused cell death lesions.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- ectopic expression of the yellow fluorescent protein (YFP)-tagged RPW8.1 from the native promotere0
- Gene
- RPW8.1e0
- Outcome
- cell death lesionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of the yellow fluorescent protein (YFP)-tagged RPW8.1 from the native promoter leads to unique cell death lesions”
t0“Arabidopsis”
Claim · model-written summary
RPW8.1 expression enhanced resistance to fungal and oomycete pathogens.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Ectopic Expression of RESISTANCE TO POWDERY MILDEW8.1t0
- Outcome
- resistance to virulent fungal and oomycete pathogense0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhances resistance to virulent fungal and oomycete pathogens that cause powdery mildew and downy mildew diseases”
t0“Ectopic Expression of RESISTANCE TO POWDERY MILDEW8.1 Confers Resistance to Fungal and Oomycete Pathogens in Arabidopsis”
Claim · model-written summary
RPW8.1-YFP accumulated at higher levels in mesophyll cells.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- mesophyll cellse0
- Outcome
- RPW8.1-YFP accumulatese0
- Direction
- higher levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPW8.1-YFP accumulates at higher levels in the mesophyll cells underneath the infected epidermal cells”
t0“Arabidopsis”
Claim · model-written summary
RPW8.1-YFP was targeted to the EHM under the RPW8.2 promoter.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ectopically expressed from the RPW8.2 promotere0
- Outcome
- targeted to the EHM of powdery mildewe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when ectopically expressed from the RPW8.2 promoter, RPW8.1-YFP is also targeted to the EHM of powdery mildew”
t0“Arabidopsis”
Claim · model-written summary
The RPW8.1 promoter was constitutively active and induced at infection sites.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RPW8.1 promotere0
- Outcome
- constitutively activee0
- Direction
- higher levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the RPW8.1 promoter is constitutively active but induced to higher levels in cells at the infection site”
t0“Arabidopsis”
Claim · model-written summary
The RPW8.2 promoter was activated specifically at infection sites.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RPW8.2 promotere0
- Outcome
- activated specifically in cells at the infection sitee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the RPW8.2 promoter is activated specifically in cells at the infection site”
t0“Arabidopsis”
The same questions in other papers
overexpression · cytology · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →overexpression · disease · ArabidopsisPositive and negative results
55 papers in the same direction as this paper · 10 in the opposite direction
- positive
- negative
- negative
62 papers more in this group
See all 66 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.