Abstract
The Arabidopsis gene RESISTANCE TO POWDERY MILDEW8.1 (RPW8.1) confers resistance to virulent fungal and oomycete pathogens that cause powdery mildew and downy mildew, respectively. However, the underlying mechanism remains unclear. Here, we show that ectopic expression of RPW8.1 boosts pattern-triggered immunity (PTI) resulting in enhanced resistance against different pathogens in both Arabidopsis and rice. In Arabidopsis, transcriptome analysis revealed that ectopic expression of RPW8.1-YFP constitutively up-regulates expression of many pathogen-associated molecular pattern (PAMP-)-inducible genes. Consistently, upon PAMP application, the transgenic line expressing RPW8.1-YFP exhibited more pronounced PTI responses such as callose deposition, production of reactive oxygen species, expression of defence-related genes and hypersensitive response-like cell death. Accordingly, the growth of a virulent bacterial pathogen was significantly inhibited in the transgenic lines expressing RPW8.1-YFP. Conversely, impairment of the PTI signalling pathway from PAMP cognition to the immediate downstream relay of phosphorylation abolished or significantly compromised RPW8.1-boosted PTI responses. In rice, heterologous expression of RPW8.1-YFP also led to enhanced resistance to the blast fungus Pyricularia oryzae (syn. Magnaporthe oryzae) and the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). Taken together, our data suggest a surprising mechanistic connection between RPW8.1 function and PTI, and demonstrate the potential of RPW8.1 as a transgene for engineering disease resistance across wide taxonomic lineages of plants.
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Claims 8
Claim · model-written summary
Ectopic RPW8.1 expression enhanced pattern-triggered immunity in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- ectopic expression of RPW8.1e0
- Gene
- RPW8.1e0
- Outcome
- pattern-triggered immunity (PTI)e0
- Direction
- boostse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of RPW8.1 boosts pattern-triggered immunity (PTI) resulting in enhanced resistance against different pathogens in both Arabidopsis and rice.”
Claim · model-written summary
Ectopic RPW8.1 expression enhanced pathogen resistance in rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- ectopic expression of RPW8.1e0
- Gene
- RPW8.1e0
- Outcome
- resistance against different pathogense0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of RPW8.1 boosts pattern-triggered immunity (PTI) resulting in enhanced resistance against different pathogens in both Arabidopsis and rice.”
Claim · model-written summary
Arabidopsis RPW8.1-YFP expression constitutively increased many PAMP-inducible transcripts.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ectopic expression of RPW8.1-YFPe0
- Gene
- RPW8.1-YFPe0
- Outcome
- expression of many pathogen-associated molecular pattern (PAMP-)-inducible genese0
- Direction
- up-regulatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ectopic expression of RPW8.1-YFP constitutively up-regulates expression of many pathogen-associated molecular pattern (PAMP-)-inducible genes.”
t0“Arabidopsis”
Claim · model-written summary
After PAMP application, RPW8.1-YFP Arabidopsis showed stronger callose, ROS, defence gene, and cell-death responses.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic line expressing RPW8.1-YFPe0
- Treatment
- PAMP applicatione0
- Outcome
- callose deposition, production of reactive oxygen species, expression of defence-related genes and hypersensitive response-like cell deathe0
- Direction
- more pronouncede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“upon PAMP application, the transgenic line expressing RPW8.1-YFP exhibited more pronounced PTI responses such as callose deposition, production of reactive oxygen species, expression of defence-related genes and hypersensitive response-like cell death.”
t0“Arabidopsis”
Claim · model-written summary
Virulent bacterial pathogen growth was significantly inhibited in RPW8.1-YFP Arabidopsis lines.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenic lines expressing RPW8.1-YFPe0
- Interactor
- virulent bacterial pathogene0pathogen
- Outcome
- growth of a virulent bacterial pathogene0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the growth of a virulent bacterial pathogen was significantly inhibited in the transgenic lines expressing RPW8.1-YFP.”
t0“Arabidopsis”
Claim · model-written summary
Disrupting PTI signalling abolished or compromised RPW8.1-enhanced PTI responses.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- impairment of the PTI signalling pathwaye0
- Outcome
- RPW8.1-boosted PTI responsese0
- Direction
- abolished or significantly compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“impairment of the PTI signalling pathway from PAMP cognition to the immediate downstream relay of phosphorylation abolished or significantly compromised RPW8.1-boosted PTI responses.”
t0“Arabidopsis”
Claim · model-written summary
RPW8.1-YFP expression enhanced rice resistance to Pyricularia oryzae.
- Taxon
- riceOryza sativat1
- Treatment
- heterologous expression of RPW8.1-YFPe0
- Interactor
- Pyricularia oryzae
Pyricularia oryzaee0pathogen - Gene
- RPW8.1-YFPe0
- Outcome
- resistance to the blast fungus Pyricularia oryzaee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous expression of RPW8.1-YFP also led to enhanced resistance to the blast fungus Pyricularia oryzae (syn. Magnaporthe oryzae) and the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo).”
t1“rice”
Claim · model-written summary
RPW8.1-YFP expression enhanced rice resistance to Xanthomonas oryzae pv. oryzae.
- Taxon
- riceOryza sativat1
- Treatment
- heterologous expression of RPW8.1-YFPe0
- Interactor
- Xanthomonas oryzae pv. oryzae
Xanthomonas oryzae pv. oryzaee0pathogen - Gene
- RPW8.1-YFPe0
- Outcome
- resistance to the blast fungus Pyricularia oryzaee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous expression of RPW8.1-YFP also led to enhanced resistance to the blast fungus Pyricularia oryzae (syn. Magnaporthe oryzae) and the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo).”
t1“rice”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.