Abstract
Abstract MicroRNAs (miRNAs) play essential roles in rice immunity against Magnaporthe oryzae , the causative agent of rice blast disease. Here we demonstrate that Osa-miR162a fine-tunes rice immunity against M. oryzae and yield traits. Overexpression of Osa-miR162a enhances rice resistance to M. oryzae accompanying enhanced induction of defense-related genes and accumulation of hydrogen peroxide (H 2 O 2 ). In contrast, blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162a enhances susceptibility to blast fungus associating with compromised induction of defense-related gene expression and H 2 O 2 accumulation. Moreover, the transgenic lines overexpressing Osa-miR162a display decreased seed setting rate resulting in slight reduced yield per plant, whereas the transgenic lines blocking Osa-miR162 show an increased number of grains per panicle, resulting in increased yield per plant. Altered accumulation of Osa-miR162 had a limited impact on the expression of rice Dicer-like 1 ( OsDCL1 ) in these transgenic lines showing normal gross morphology, and silencing of OsDCL1 led to enhanced resistance to blast fungus similar to that caused by overexpression of Osa-miR162a, suggesting the involvement of OsDCL1 in Osa-miR162a-regulated resistance. Together, our results indicate that Osa-miR162a is involved in rice immunity against M. oryzae and fine-tunes resistance and yield.
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Claims 12
Claim · model-written summary
Overexpressing Osa-miR162a enhanced rice resistance to M. oryzae.
- Taxon
- rice
Oryza sativae0 - Treatment
- Overexpression of Osa-miR162ae0
- Interactor
- M. oryzae
Pyricularia oryzaee0pathogen - Gene
- Osa-miR162ae0
- Outcome
- resistance to M. oryzaee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of Osa-miR162a enhances rice resistance to M. oryzae accompanying enhanced induction of defense-related genes and accumulation of hydrogen peroxide (H 2 O 2 ).”
Claim · model-written summary
Overexpressing Osa-miR162a enhanced induction of defense-related genes and hydrogen peroxide accumulation.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of Osa-miR162ae0
- Gene
- Osa-miR162ae0
- Outcome
- induction of defense-related genes and accumulation of hydrogen peroxide (H 2 O 2 )e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of Osa-miR162a enhances rice resistance to M. oryzae accompanying enhanced induction of defense-related genes and accumulation of hydrogen peroxide (H 2 O 2 ).”
Claim · model-written summary
Blocking Osa-miR162 through a target mimic increased rice susceptibility to blast fungus.
- Taxon
- riceOryza sativat0
- Treatment
- blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162ae0
- Interactor
- blast funguse0pathogen
- Gene
- Osa-miR162e0
- Outcome
- susceptibility to blast funguse0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162a enhances susceptibility to blast fungus associating with compromised induction of defense-related gene expression and H 2 O 2 accumulation.”
t0“rice resistance”
Claim · model-written summary
Blocking Osa-miR162 compromised defense gene induction and hydrogen peroxide accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162ae0
- Gene
- Osa-miR162e0
- Outcome
- induction of defense-related gene expression and H 2 O 2 accumulatione0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“blocking Osa-miR162 by overexpressing a target mimic of Osa-miR162a enhances susceptibility to blast fungus associating with compromised induction of defense-related gene expression and H 2 O 2 accumulation.”
t0“rice resistance”
Claim · model-written summary
Osa-miR162a-overexpressing lines had a decreased seed setting rate.
- Taxon
- riceOryza sativat0
- Treatment
- overexpressing Osa-miR162ae0
- Outcome
- seed setting ratee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic lines overexpressing Osa-miR162a display decreased seed setting rate resulting in slight reduced yield per plant”
t0“rice resistance”
Claim · model-written summary
Osa-miR162a-overexpressing lines had slightly reduced yield per plant.
- Taxon
- riceOryza sativat0
- Treatment
- overexpressing Osa-miR162ae0
- Outcome
- yield per plante0
- Direction
- reducede0
- Qualifier
- slighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic lines overexpressing Osa-miR162a display decreased seed setting rate resulting in slight reduced yield per plant”
t0“rice resistance”
Claim · model-written summary
Lines blocking Osa-miR162 had more grains per panicle.
- Taxon
- riceOryza sativat0
- Treatment
- blocking Osa-miR162e0
- Outcome
- number of grains per paniclee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic lines blocking Osa-miR162 show an increased number of grains per panicle, resulting in increased yield per plant.”
t0“rice resistance”
Claim · model-written summary
Lines blocking Osa-miR162 had increased yield per plant.
- Taxon
- riceOryza sativat0
- Treatment
- blocking Osa-miR162e0
- Outcome
- yield per plante0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic lines blocking Osa-miR162 show an increased number of grains per panicle, resulting in increased yield per plant.”
t0“rice resistance”
Claim · model-written summary
Altered Osa-miR162 accumulation had limited impact on OsDCL1 expression.
- Taxon
- riceOryza sativae0
- Gene
- Osa-miR162e0
- Outcome
- expression of rice Dicer-like 1 ( OsDCL1 )e0
- Direction
- limited impacte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Altered accumulation of Osa-miR162 had a limited impact on the expression of rice Dicer-like 1 ( OsDCL1 ) in these transgenic lines showing normal gross morphology”
Claim · model-written summary
Transgenic lines with altered Osa-miR162 showed normal gross morphology.
- Taxon
- riceOryza sativat0
- Outcome
- normal gross morphologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these transgenic lines showing normal gross morphology”
t0“rice resistance”
Claim · model-written summary
OsDCL1 silencing enhanced resistance to blast fungus.
- Taxon
- riceOryza sativat0
- Treatment
- silencing of OsDCL1e0
- Interactor
- blast funguse0pathogen
- Gene
- OsDCL1e0
- Outcome
- resistance to blast funguse0
- Direction
- enhancede0
- Comparator
- overexpression of Osa-miR162ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of OsDCL1 led to enhanced resistance to blast fungus similar to that caused by overexpression of Osa-miR162a”
t0“rice resistance”
Claim · model-written summary
The authors infer OsDCL1 involvement in Osa-miR162a-regulated resistance.
- Taxon
- riceOryza sativat0
- Gene
- OsDCL1e0
- Outcome
- Osa-miR162a-regulated resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting the involvement of OsDCL1 in Osa-miR162a-regulated resistance”
t0“rice resistance”
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