Abstract
Exploring the regulatory mechanism played by endogenous rice miRNAs in defense responses against the blast disease is of great significance in both resistant variety breeding and disease control management. We identified rice defense-related miRNAs by comparing rice miRNA expression patterns before and after Magnaporthe oryzae strain Guy11 infection. We discovered that osa-miR164a expression reduced upon Guy11 infection at both early and late stages, which was perfectly associated with the induced expression of its target gene, OsNAC60. OsNAC60 encodes a transcription factor, over-expression of which enhanced defense responses, such as increased programmed cell death, greater ion leakage, more reactive oxygen species accumulation and callose deposition, and upregulation of defense-related genes. By using transgenic rice over-expressing osa-miR164a, and a transposon insertion mutant of OsNAC60, we showed that when the miR164a/OsNAC60 regulatory module was dysfunctional, rice developed significant susceptibility to Guy11 infection. The co-expression of OsNAC60 and osa-miR164a abolished the OsNAC60 activity, but not its synonymous mutant. We further validated that this regulatory module is conserved in plant resistance to multiple plant diseases, such as the rice sheath blight, tomato late blight, and soybean root and stem rot diseases. Our results demonstrate that the miR164a/OsNAC60 regulatory module manipulates rice defense responses to M. oryzae infection. This discovery is of great potential for resistant variety breeding and disease control to a broad spectrum of pathogens in the future.
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Claims 9
Claim · model-written summary
osa-miR164a expression declined after Guy11 infection at early and late stages.
- Taxon
- rice
Oryza sativat0 - Treatment
- Guy11 infectione0
- Interactor
- Magnaporthe oryzae
Pyricularia oryzaet0pathogen - Gene
- osa-miR164ae0
- Outcome
- osa-miR164a expressione0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osa-miR164a expression reduced upon Guy11 infection at both early and late stages”
t0“rice immunity against the blast fungus Magnaporthe oryzae”
Claim · model-written summary
OsNAC60 expression was induced as osa-miR164a expression declined after infection.
- Taxon
- riceOryza sativat0
- Treatment
- Guy11 infectione1
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Gene
- OsNAC60e0
- Outcome
- expressione0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which was perfectly associated with the induced expression of its target gene, OsNAC60”
e1“osa-miR164a expression reduced upon Guy11 infection at both early and late stages”
t0“rice immunity against the blast fungus Magnaporthe oryzae”
Claim · model-written summary
OsNAC60 overexpression increased programmed cell death, ion leakage and reactive oxygen species accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- over-expressione0
- Gene
- OsNAC60e1
- Outcome
- programmed cell death, greater ion leakage, more reactive oxygen species accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of which enhanced defense responses, such as increased programmed cell death, greater ion leakage, more reactive oxygen species accumulation and callose deposition, and upregulation of defense-related genes.”
e1“OsNAC60 encodes a transcription factor”
t0“rice immunity against the blast fungus Magnaporthe oryzae”
Claim · model-written summary
OsNAC60 overexpression increased callose deposition and defense-related gene expression.
- Taxon
- riceOryza sativat0
- Treatment
- over-expressione0
- Gene
- OsNAC60e1
- Outcome
- callose deposition, and upregulation of defense-related genese0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of which enhanced defense responses, such as increased programmed cell death, greater ion leakage, more reactive oxygen species accumulation and callose deposition, and upregulation of defense-related genes.”
e1“OsNAC60 encodes a transcription factor”
t0“rice immunity against the blast fungus Magnaporthe oryzae”
Claim · model-written summary
Disrupting the miR164a/OsNAC60 module made rice significantly susceptible to Guy11.
- Taxon
- riceOryza sativae0
- Treatment
- transgenic rice over-expressing osa-miR164a, and a transposon insertion mutant of OsNAC60e1
- Interactor
- Magnaporthe oryzaePyricularia oryzaet0pathogen
- Gene
- miR164a/OsNAC60e0
- Outcome
- susceptibility to Guy11 infectione0
- Direction
- susceptibilitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when the miR164a/OsNAC60 regulatory module was dysfunctional, rice developed significant susceptibility to Guy11 infection”
e1“transgenic rice over-expressing osa-miR164a, and a transposon insertion mutant of OsNAC60”
t0“blast fungus Magnaporthe oryzae”
Claim · model-written summary
Coexpression of OsNAC60 and osa-miR164a abolished OsNAC60 activity, unlike its synonymous mutant.
- Taxon
- riceOryza sativat0
- Treatment
- co-expression of OsNAC60 and osa-miR164ae0
- Gene
- OsNAC60 and osa-miR164ae0
- Outcome
- OsNAC60 activitye0
- Direction
- abolishede0
- Comparator
- its synonymous mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The co-expression of OsNAC60 and osa-miR164a abolished the OsNAC60 activity, but not its synonymous mutant.”
t0“rice immunity against the blast fungus Magnaporthe oryzae”
Claim · model-written summary
The regulatory module was conserved in resistance to rice sheath blight.
- Taxon
- riceOryza sativae0
- Gene
- this regulatory modulee0
- Outcome
- plant resistance to multiple plant diseasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this regulatory module is conserved in plant resistance to multiple plant diseases, such as the rice sheath blight, tomato late blight, and soybean root and stem rot diseases.”
Claim · model-written summary
The regulatory module was conserved in resistance to tomato late blight.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- this regulatory modulee0
- Outcome
- tomato late blighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this regulatory module is conserved in plant resistance to multiple plant diseases, such as the rice sheath blight, tomato late blight, and soybean root and stem rot diseases.”
Claim · model-written summary
The regulatory module was conserved in resistance to soybean root and stem rot.
- Taxon
- soybean
Glycine maxe0 - Gene
- this regulatory modulee0
- Outcome
- soybean root and stem rot diseasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this regulatory module is conserved in plant resistance to multiple plant diseases, such as the rice sheath blight, tomato late blight, and soybean root and stem rot diseases.”
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See all 67 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.