Abstract
Rice ARGONAUTE2 (OsAGO2) is a core component of the rice RNA-induced silencing complex (RISC), which is repressed by Magnaporthe oryzae (M. oryzae) infection. Whether and how OsAGO2-mediated gene silencing plays a role in rice blast resistance and which sRNAs participate in this process are unknown. Our results indicate that OsAGO2 is a key immune player that manipulates rice defense responses against blast disease. OsAGO2 associates with the 24-nt miR1875 and binds to the promoter region of HEXOKINASE1 (OsHXK1), which causes DNA methylation and leads to gene silencing. Our multiple genetic evidence showed that, without M. oryzae infection, OsAGO2/miR1875 RISC promoted OsHXK1 promoter DNA methylation and OsHXK1 silencing; after M. oryzae infection, the reduced OsAGO2/miR1875 led to a relatively activated OsHXK1 expression. OsHXK1 acts as a positive regulator of blast disease resistance that OsHXK1-OE rice exhibited enhanced resistance, whereas Cas9-Oshxk1 rice showed reduced resistance against M. oryzae infection. OsHXK1 may function through its sugar sensor activity as glucose induced defense-related gene expression and reactive oxygen species (ROS) accumulation in Nipponbare and OsHXK1-OE but not in Cas9-Oshxk1 rice. OsAGO2 itself is delicately regulated by OsPRMT5, which senses M. oryzae infection and attenuates OsAGO2-mediated gene silencing through OsAGO2 arginine methylation. Our study reveals an OsPRMT5-OsAGO2/miR1875-OsHXK1 regulatory module that fine tunes the rice defense response to blast disease.
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Claims 8
Claim · model-written summary
OsAGO2 is a key immune player that manipulates rice defense responses against blast disease.
- Taxon
- rice
Oryza sativae0 - Gene
- OsAGO2e0
- Outcome
- defense responses against blast diseasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsAGO2 is a key immune player that manipulates rice defense responses against blast disease”
Claim · model-written summary
Without M. oryzae infection, OsAGO2/miR1875 RISC promoted OsHXK1 promoter DNA methylation and OsHXK1 silencing in rice.
- Taxon
- riceOryza sativat0
- Interactor
- M. oryzae
Pyricularia oryzaee0pathogen - Gene
- OsAGO2/miR1875 RISCe0
- Outcome
- OsHXK1 promoter DNA methylation and OsHXK1 silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“without M. oryzae infection, OsAGO2/miR1875 RISC promoted OsHXK1 promoter DNA methylation and OsHXK1 silencing”
t0“rice”
Claim · model-written summary
After M. oryzae infection, reduced OsAGO2/miR1875 led to relatively activated OsHXK1 expression in rice.
- Taxon
- riceOryza sativat0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Gene
- OsAGO2/miR1875e0
- Outcome
- OsHXK1 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after M. oryzae infection, the reduced OsAGO2/miR1875 led to a relatively activated OsHXK1 expression”
t0“rice”
Claim · model-written summary
OsHXK1-overexpressing rice exhibited enhanced blast disease resistance.
- Taxon
- riceOryza sativae0
- Treatment
- OsHXK1-OEe0
- Gene
- OsHXK1e0
- Outcome
- blast disease resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHXK1 acts as a positive regulator of blast disease resistance that OsHXK1-OE rice exhibited enhanced resistance”
Claim · model-written summary
Cas9-Oshxk1 rice showed reduced resistance against M. oryzae infection.
- Taxon
- riceOryza sativae0
- Treatment
- Cas9-Oshxk1e0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Outcome
- resistancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cas9-Oshxk1 rice showed reduced resistance against M. oryzae infection”
Claim · model-written summary
Glucose induced defense-related gene expression and ROS accumulation in Nipponbare and OsHXK1-OE rice but not in Cas9-Oshxk1 rice.
- Taxon
- riceOryza sativae0
- Material
- Nipponbaree0
- Treatment
- glucosee0
- Outcome
- defense-related gene expression and reactive oxygen species (ROS) accumulatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucose induced defense-related gene expression and reactive oxygen species (ROS) accumulation in Nipponbare and OsHXK1-OE but not in Cas9-Oshxk1 rice”
Claim · model-written summary
OsHXK1 may function through its sugar sensor activity in rice defense.
- Taxon
- riceOryza sativat0
- Gene
- OsHXK1e0
- Outcome
- function through its sugar sensor activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHXK1 may function through its sugar sensor activity”
t0“rice”
Claim · model-written summary
OsAGO2 is regulated by OsPRMT5, which senses M. oryzae infection and attenuates OsAGO2-mediated gene silencing through OsAGO2 arginine methylation.
- Taxon
- riceOryza sativat0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Gene
- OsPRMT5e0
- Outcome
- OsAGO2-mediated gene silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsAGO2 itself is delicately regulated by OsPRMT5, which senses M. oryzae infection and attenuates OsAGO2-mediated gene silencing through OsAGO2 arginine methylation”
t0“rice”
The same questions in other papers
loss of function · Pyricularia oryzae · Oryza sativa (rice)Positive and negative results
25 papers in the same direction as this paper · 41 in the opposite direction
- negative
- positive
- positive
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