Claim · model-written summary
Arabidopsis transformed with French bean peroxidase in antisense orientation became highly susceptible to bacterial and fungal pathogens.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- transformed heterologously using the French bean peroxidase in antisense orientatione0
- Gene
- French bean peroxidasee0
- Outcome
- susceptible to bacterial and fungal pathogense0
Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis plants transformed heterologously using the French bean peroxidase in antisense orientation have proved to be highly susceptible to bacterial and fungal pathogens”
Title
The apoplastic oxidative burst in response to biotic stress in plants: a three-component system
G. P. Bolwell Journal of Experimental Botany 2002. doi:10.1093/jexbot/53.372.1367
Other claims from this paper (7)
- Observation NADPH oxidases have been implicated in oxidative burst systems and characterized.
- Mechanism An exocellular peroxidase appears to be the main ROS source in elicited French bean cells.
- Mechanism Extracellular alkalinization follows calcium and proton influx and potassium efflux in elicitation systems.
- Observation The candidate substrate becomes available minutes after elicitation and is extractable.
- Intervention effect Palmitate and stearate stimulated hydrogen peroxide production without oxylipin production.
- Observation The peroxidase isoform occurs at hydrogen peroxide production sites in infection papillae alongside cross-linked wall proteins, callose and callose synthase.
- Observation Cloned French bean peroxidase expressed in Pichia catalysed oxidation with the kinetics of the purified enzyme.