Abstract
A transgenic Arabidopsis line containing a chimeric PR-1::luciferase (LUC) reporter gene was subjected to mutagenesis with activation tags. Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression. Here, we report the characterization of one of these mutants, designated activated disease resistance (adr) 1. This line showed constitutive expression of a number of key defense marker genes and accumulated salicylic acid but not ethylene or jasmonic acid. Furthermore, adr1 plants exhibited resistance against the biotrophic pathogens Peronospora parasitica and Erysiphe cichoracearum but not the necrotrophic fungus Botrytis cinerea. Analysis of a series of adr1 double mutants suggested that adr1-mediated resistance against P. parasitica was salicylic acid (SA)-dependent, while resistance against E. cichoracearum was both SA-dependent and partially NPR1-dependent. The ADR1 gene encoded a protein possessing a number of key features, including homology to subdomains of protein kinases, a nucleotide binding domain, and leucine-rich repeats. The controlled, transient expression of ADR1 conveyed striking disease resistance in the absence of yield penalty, highlighting the potential utility of this gene in crop protection.
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Claims 11
Claim · model-written summary
Dominant Arabidopsis mutants identified by screening showed enhanced PR-1 gene expression
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- dominant Arabidopsis mutantse0
- Gene
- PR-1e0
- Outcome
- PR-1 gene expressione0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
Claim · model-written summary
The adr1 line showed constitutive expression of key defence marker genes
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- constitutive expression of a number of key defense marker genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“This line showed constitutive expression of a number of key defense marker genes and accumulated salicylic acid but not ethylene or jasmonic acid”
Claim · model-written summary
The adr1 line accumulated salicylic acid
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- accumulated salicylic acide1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“This line showed constitutive expression of a number of key defense marker genes and accumulated salicylic acid but not ethylene or jasmonic acid”
Claim · model-written summary
The adr1 line did not accumulate ethylene or jasmonic acid
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- ethylene or jasmonic acide1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“This line showed constitutive expression of a number of key defense marker genes and accumulated salicylic acid but not ethylene or jasmonic acid”
Claim · model-written summary
adr1 plants showed resistance against Peronospora parasitica
- Taxon
- ArabidopsisArabidopsise0
- Material
- adr1 plantse1
- Interactor
- Peronospora parasitica
Hyaloperonospora parasiticae1pathogen - Outcome
- resistancee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“adr1 plants exhibited resistance against the biotrophic pathogens Peronospora parasitica and Erysiphe cichoracearum but not the necrotrophic fungus Botrytis cinerea”
Claim · model-written summary
adr1 plants showed resistance against Erysiphe cichoracearum
- Taxon
- ArabidopsisArabidopsise0
- Material
- adr1 plantse1
- Interactor
- Erysiphe cichoracearum
Golovinomyces cichoracearume1pathogen - Outcome
- resistancee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“adr1 plants exhibited resistance against the biotrophic pathogens Peronospora parasitica and Erysiphe cichoracearum but not the necrotrophic fungus Botrytis cinerea”
Claim · model-written summary
adr1 plants showed no resistance against Botrytis cinerea
- Taxon
- ArabidopsisArabidopsise0
- Material
- adr1 plantse1
- Interactor
- Botrytis cinerea
Botrytis cinereae1pathogen - Outcome
- resistancee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“adr1 plants exhibited resistance against the biotrophic pathogens Peronospora parasitica and Erysiphe cichoracearum but not the necrotrophic fungus Botrytis cinerea”
Claim · model-written summary
adr1-mediated resistance to P. parasitica is salicylic acid dependent
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- P. parasiticaHyaloperonospora parasiticae1pathogen
- Compound
- salicylic acid (SA)e1
- Outcome
- resistance against P. parasiticae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“adr1-mediated resistance against P. parasitica was salicylic acid (SA)-dependent”
Claim · model-written summary
Resistance to E. cichoracearum is both SA dependent and partially NPR1 dependent
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- E. cichoracearumGolovinomyces cichoracearume1pathogen
- Gene
- NPR1e1
- Compound
- SAe1
- Outcome
- resistance against E. cichoracearume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“resistance against E. cichoracearum was both SA-dependent and partially NPR1-dependent”
Claim · model-written summary
ADR1 encodes a protein with homology to protein kinase subdomains, a nucleotide binding domain and leucine-rich repeats
- Taxon
- ArabidopsisArabidopsise0
- Gene
- ADR1e1
- Outcome
- homology to subdomains of protein kinases, a nucleotide binding domain, and leucine-rich repeatse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“The ADR1 gene encoded a protein possessing a number of key features, including homology to subdomains of protein kinases, a nucleotide binding domain, and leucine-rich repeats”
Claim · model-written summary
Transient expression of ADR1 increased disease resistance without yield penalty
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- transient expression of ADR1e1
- Gene
- ADR1e1
- Outcome
- disease resistancee1
- Direction
- increaseinferrede1
- Qualifier
- in the absence of yield penaltye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of lines via high-throughput LUC imaging identified a number of dominant Arabidopsis mutants that exhibited enhanced PR-1 gene expression”
e1“The controlled, transient expression of ADR1 conveyed striking disease resistance in the absence of yield penalty”
The same questions in other papers
transgenic · disease · ArabidopsisPositive and negative results
35 papers in the same direction as this paper · 19 in the opposite direction
- no effect
- negative
- negative
51 papers more in this group
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