Abstract
The Jerusalem artichoke (Helianthus tuberosus) xenobiotic inducible cytochrome P450, CYP76B1, catalyzes rapid oxidative dealkylation of various phenylurea herbicides to yield nonphytotoxic metabolites. We have found that increased herbicide metabolism and tolerance can be achieved by ectopic constitutive expression of CYP76B1 in tobacco (Nicotiana tabacum) and Arabidopsis. Transformation with CYP76B1 conferred on tobacco and Arabidopsis a 20-fold increase in tolerance to linuron, a compound detoxified by a single dealkylation, and a 10-fold increase in tolerance to isoproturon or chlortoluron, which need successive catalytic steps for detoxification. Two constructs for expression of translational fusions of CYP76B1 with P450 reductase were prepared to test if they would yield even greater herbicide tolerance. Plants expressing these constructs had lower herbicide tolerance than CYP76B1 alone, which is apparently a consequence of reduced stability of the fusion proteins. In all cases, increased herbicide tolerance results from more extensive metabolism, as demonstrated with exogenously fed phenylurea. Beside increased herbicide tolerance, expression of CYP76B1 has no other visible phenotype in the transgenic plants. Our data indicate that CYP76B1 can function as a selectable marker for plant transformation, allowing efficient selection in vitro and in soil-grown plants. Plants expressing CYP76B1 may also be a potential tool for phytoremediation of contaminated sites.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
CYP76B1 catalyzed rapid oxidative dealkylation of phenylurea herbicides into nonphytotoxic metabolites.
- Taxon
- Helianthus tuberosus
.jpg?width=60)
Helianthus tuberosuse0 - Gene
- CYP76B1e0
- Outcome
- oxidative dealkylation of various phenylurea herbicidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The Jerusalem artichoke (Helianthus tuberosus) xenobiotic inducible cytochrome P450, CYP76B1, catalyzes rapid oxidative dealkylation of various phenylurea herbicides to yield nonphytotoxic metabolites.”
Claim · model-written summary
CYP76B1 transformation increased tobacco tolerance to linuron 20-fold.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Transformation with CYP76B1e0
- Gene
- CYP76B1e0
- Outcome
- tolerance to linurone0
- Direction
- increasee0
- Value
- 20-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation with CYP76B1 conferred on tobacco and Arabidopsis a 20-fold increase in tolerance to linuron”
Claim · model-written summary
CYP76B1 transformation increased Arabidopsis tolerance to linuron 20-fold.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Transformation with CYP76B1e0
- Gene
- CYP76B1e0
- Outcome
- tolerance to linurone0
- Direction
- increasee0
- Value
- 20-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation with CYP76B1 conferred on tobacco and Arabidopsis a 20-fold increase in tolerance to linuron”
Claim · model-written summary
CYP76B1 transformation increased tobacco tolerance to isoproturon or chlortoluron 10-fold.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Transformation with CYP76B1e0
- Gene
- CYP76B1e0
- Outcome
- tolerance to isoproturon or chlortolurone0
- Direction
- increasee0
- Value
- 10-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation with CYP76B1 conferred on tobacco and Arabidopsis a 20-fold increase in tolerance to linuron, a compound detoxified by a single dealkylation, and a 10-fold increase in tolerance to isoproturon or chlortoluron”
Claim · model-written summary
CYP76B1 transformation increased Arabidopsis tolerance to isoproturon or chlortoluron 10-fold.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Transformation with CYP76B1e0
- Gene
- CYP76B1e0
- Outcome
- tolerance to isoproturon or chlortolurone0
- Direction
- increasee0
- Value
- 10-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation with CYP76B1 conferred on tobacco and Arabidopsis a 20-fold increase in tolerance to linuron, a compound detoxified by a single dealkylation, and a 10-fold increase in tolerance to isoproturon or chlortoluron”
Claim · model-written summary
Plants expressing CYP76B1–P450 reductase fusions had lower herbicide tolerance than those expressing CYP76B1 alone.
Verbatim
e0“Plants expressing these constructs had lower herbicide tolerance than CYP76B1 alone”
Claim · model-written summary
Reduced stability of the fusion proteins apparently explains their lower herbicide tolerance.
- Taxon
- PlantsViridiplantaee0
- Outcome
- lower herbicide tolerancee0
- Qualifier
- reduced stability of the fusion proteinse0
Verbatim
e0“Plants expressing these constructs had lower herbicide tolerance than CYP76B1 alone, which is apparently a consequence of reduced stability of the fusion proteins.”
Claim · model-written summary
More extensive phenylurea metabolism accounted for the increased herbicide tolerance in CYP76B1 plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- increased herbicide tolerancee0
- Qualifier
- more extensive metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased herbicide tolerance results from more extensive metabolism, as demonstrated with exogenously fed phenylurea.”
t0“Tobacco”
Claim · model-written summary
CYP76B1 expression produced no other visible phenotype in transgenic plants.
Verbatim
e0“expression of CYP76B1 has no other visible phenotype in the transgenic plants.”
Claim · model-written summary
The authors propose CYP76B1 as a selectable marker for transformation in vitro and in soil-grown plants.
Verbatim
e0“CYP76B1 can function as a selectable marker for plant transformation, allowing efficient selection in vitro and in soil-grown plants.”
Claim · model-written summary
The authors propose CYP76B1-expressing plants as a potential phytoremediation tool.
- Taxon
- PlantsViridiplantaee0
- Gene
- CYP76B1e0
- Outcome
- potential tool for phytoremediation of contaminated sitese0
Verbatim
e0“Plants expressing CYP76B1 may also be a potential tool for phytoremediation of contaminated sites.”
The same questions in other papers
transgenic · disease · Nicotiana tabacum (tobacco)Positive and negative results
33 papers in the same direction as this paper · 12 in the opposite direction
- negative
- negative
- no effect
42 papers more in this group
See all 46 papers →transgenic · disease · ArabidopsisPositive and negative results
35 papers in the same direction as this paper · 19 in the opposite direction
- no effect
- negative
- negative
51 papers more in this group
See all 55 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.