Abstract
Interactions between rice and Magnaporthe oryzae involve the recognition of cellular components and the exchange of complex molecular signals from both partners. How these interactions occur in rice cells is still elusive. We employed robust-long serial analysis of gene expression, massively parallel signature sequencing, and sequencing by synthesis to examine transcriptome profiles of infected rice leaves. A total of 6,413 in planta-expressed fungal genes, including 851 genes encoding predicted effector proteins, were identified. We used a protoplast transient expression system to assess 42 of the predicted effector proteins for the ability to induce plant cell death. Ectopic expression assays identified five novel effectors that induced host cell death only when they contained the signal peptide for secretion to the extracellular space. Four of them induced cell death in Nicotiana benthamiana. Although the five effectors are highly diverse in their sequences, the physiological basis of cell death induced by each was similar. This study demonstrates that our integrative genomic approach is effective for the identification of in planta-expressed cell death-inducing effectors from M. oryzae that may play an important role facilitating colonization and fungal growth during infection.
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Claims 6
Claim · model-written summary
A total of 6,413 in planta-expressed fungal genes were identified in infected rice leaves.
- Taxon
- rice
Oryza sativae0 - Interactor
- Magnaporthe oryzae
Pyricularia oryzaee0pathogen - Outcome
- in planta-expressed fungal genese2
- Value
- 6,413e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interactions between rice and Magnaporthe oryzae”
e1“transcriptome profiles of infected rice leaves”
e2“A total of 6,413 in planta-expressed fungal genes, including 851 genes encoding predicted effector proteins, were identified”
Claim · model-written summary
Of the in planta-expressed fungal genes, 851 encoded predicted effector proteins.
- Taxon
- riceOryza sativae0
- Interactor
- Magnaporthe oryzaePyricularia oryzaee0pathogen
- Outcome
- genes encoding predicted effector proteinse2
- Value
- 851e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interactions between rice and Magnaporthe oryzae”
e2“A total of 6,413 in planta-expressed fungal genes, including 851 genes encoding predicted effector proteins, were identified”
Claim · model-written summary
Five novel effectors induced host cell death in rice only when they contained the secretion signal peptide.
- Taxon
- riceOryza sativae0
- Treatment
- Ectopic expressione3
- Interactor
- Magnaporthe oryzaePyricularia oryzaee0pathogen
- Outcome
- host cell deathe3
- Direction
- inducede3
- Qualifier
- only when they contained the signal peptide for secretion to the extracellular spacee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interactions between rice and Magnaporthe oryzae”
e3“Ectopic expression assays identified five novel effectors that induced host cell death only when they contained the signal peptide for secretion to the extracellular space”
Claim · model-written summary
Four of the five effectors induced cell death in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae4 - Treatment
- Ectopic expressione3
- Outcome
- cell deathe4
- Direction
- inducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Ectopic expression assays identified five novel effectors that induced host cell death only when they contained the signal peptide for secretion to the extracellular space”
e4“Four of them induced cell death in Nicotiana benthamiana”
Claim · model-written summary
The five effectors, though highly diverse in sequence, induced cell death through a similar physiological basis in rice.
- Taxon
- riceOryza sativae0
- Outcome
- physiological basis of cell deathe5
- Direction
- similare5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interactions between rice and Magnaporthe oryzae”
e5“the physiological basis of cell death induced by each was similar”
Claim · model-written summary
The integrative genomic approach is effective for identifying in planta-expressed cell death-inducing effectors.
- Taxon
- riceOryza sativae0
- Outcome
- identification of in planta-expressed cell death-inducing effectorse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interactions between rice and Magnaporthe oryzae”
e6“This study demonstrates that our integrative genomic approach is effective for the identification of in planta-expressed cell death-inducing effectors”
The same questions in other papers
overexpression · cytology · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →overexpression · Pyricularia oryzae · Oryza sativa (rice)Positive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- negative
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.