Abstract
Induction of cell death is an important component of plant defense against pathogens. There have been many reports on the role of phytohormones in pathogen-induced cell death, but jasmonic acid (JA) has not been implicated as a regulator of the response. Here, we report the function of NbHB1, Nicotiana benthamiana homeobox1, in pathogen-induced cell death in connection with JA signaling. Involvement of NbHB1 in cell death was analysed by gain- and loss-of-function studies using Agrobacterium-mediated transient overexpression and virus-induced gene silencing, respectively. Expression of NbHB1 following pathogen inoculations and various treatments was monitored by reverse transcription polymerase chain reaction. Transcript levels of NbHB1 were upregulated by infection with virulent and avirulent bacterial pathogens. Ectopic expression of NbHB1 accelerated cell death following treatment with darkness, methyl jasmonate, or pathogen inoculation. Conversely, when NbHB1 was silenced, pathogen-induced cell death was delayed. NbHB1-induced cell death was also delayed by silencing of NbCOI1, indicating a requirement for JA-mediated signaling. Overexpression of the domain-deleted proteins of NbHB1 revealed that the homeodomain, leucine zipper, and part of the variable N-terminal region were necessary for NbHB1 functionality. These results strongly suggest the role of NbHB1 in pathogen-induced plant cell death via the JA-mediated signaling pathway.
Abstract from the paper’s OpenAlex record; open copy licensed other-oa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
NbHB1 transcript levels were upregulated by infection with virulent and avirulent bacterial pathogens.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Interactor
- bacterial pathogense0pathogen
- Gene
- NbHB1e0
- Outcome
- Transcript levels of NbHB1e0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of NbHB1 were upregulated by infection with virulent and avirulent bacterial pathogens”
t0“Nicotiana benthamiana”
Claim · model-written summary
Ectopic expression of NbHB1 accelerated cell death following darkness treatment.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Ectopic expression of NbHB1e0
- Outcome
- cell deathe0
- Direction
- acceleratede0
- Qualifier
- darknesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of NbHB1 accelerated cell death following treatment with darkness, methyl jasmonate, or pathogen inoculation”
t0“Nicotiana benthamiana”
Claim · model-written summary
Ectopic expression of NbHB1 accelerated cell death following methyl jasmonate treatment.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Ectopic expression of NbHB1e0
- Outcome
- cell deathe0
- Direction
- acceleratede0
- Qualifier
- methyl jasmonatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of NbHB1 accelerated cell death following treatment with darkness, methyl jasmonate, or pathogen inoculation”
t0“Nicotiana benthamiana”
Claim · model-written summary
Ectopic expression of NbHB1 accelerated cell death following pathogen inoculation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Ectopic expression of NbHB1e0
- Interactor
- pathogene0pathogen
- Outcome
- cell deathe0
- Direction
- acceleratede0
- Qualifier
- pathogen inoculatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of NbHB1 accelerated cell death following treatment with darkness, methyl jasmonate, or pathogen inoculation”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing of NbHB1 delayed pathogen-induced cell death.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencede0
- Gene
- NbHB1e0
- Outcome
- pathogen-induced cell deathe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when NbHB1 was silenced, pathogen-induced cell death was delayed”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbHB1-induced cell death was delayed by silencing of NbCOI1, indicating a requirement for JA-mediated signaling.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silencing of NbCOI1e0
- Gene
- NbCOI1e0
- Outcome
- NbHB1-induced cell deathe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbHB1-induced cell death was also delayed by silencing of NbCOI1”
t0“Nicotiana benthamiana”
Claim · model-written summary
The homeodomain, leucine zipper and part of the variable N-terminal region of NbHB1 were necessary for its functionality.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbHB1e0
- Outcome
- NbHB1 functionalitye0
- Qualifier
- homeodomain, leucine zipper, and part of the variable N-terminal regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the homeodomain, leucine zipper, and part of the variable N-terminal region were necessary for NbHB1 functionality”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors conclude that NbHB1 acts in pathogen-induced plant cell death via the JA-mediated signaling pathway.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbHB1e0
- Outcome
- pathogen-induced plant cell deathe0
- Qualifier
- JA-mediated signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the role of NbHB1 in pathogen-induced plant cell death via the JA-mediated signaling pathway”
t0“Nicotiana benthamiana”
The same questions in other papers
overexpression · cytology · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
2 papers more in this group
See all 6 papers →loss of function · cytology · Nicotiana benthamianaPositive and negative results
23 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
29 papers more in this group
See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.