Abstract
Victoria blight, caused by Cochliobolus victoriae, is a disease originally described on oat and recapitulated on Arabidopsis. C. victoriae pathogenesis depends upon production of the toxin victorin. In oat, victorin sensitivity is conferred by the Vb gene, which is genetically inseparable from the Pc2 resistance gene. Concurrently, in Arabidopsis, sensitivity is conferred by the LOCUS ORCHESTRATING VICTORIN EFFECTS1 (LOV1) gene. LOV1 encodes a nucleotide-binding site leucine-rich repeat protein, a type of protein commonly associated with disease resistance, and LOV1 "guards" the defense thioredoxin, TRX-h5. Expression of LOV1 and TRX-h5 in Nicotiana benthamiana is sufficient to confer victorin sensitivity. Virus-induced gene silencing was used to characterize victorin-induced cell death in N. benthamiana. We determined that SGT1 is required for sensitivity and involved in LOV1 protein accumulation. We screened a normalized cDNA library and identified six genes that, when silenced, suppressed LOV1-mediated, victorin-induced cell death and cell death induced by expression of the closely related RPP8 resistance gene: a mitochondrial phosphate transporter, glycolate oxidase, glutamine synthetase, glyceraldehyde 3-phosphate dehydrogenase, and the P- and T-protein of the glycine decarboxylase complex. Silencing the latter four also inhibited cell death and disease resistance mediated by the PTO resistance gene. Together, these results provide evidence that the victorin response mediated by LOV1 is a defense response.
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Claims 6
Claim · model-written summary
SGT1 is required for victorin sensitivity and involved in LOV1 protein accumulation in N. benthamiana.
- Taxon
- N. benthamiana
Nicotiana benthamianat0 - Gene
- SGT1e0
- Outcome
- sensitivitye0
- Qualifier
- LOV1 protein accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We determined that SGT1 is required for sensitivity and involved in LOV1 protein accumulation.”
t0“cell death in N. benthamiana”
Claim · model-written summary
Silencing six identified genes suppressed LOV1-mediated victorin-induced cell death.
- Taxon
- N. benthamianaNicotiana benthamianat0
- Treatment
- silencede0
- Outcome
- LOV1-mediated, victorin-induced cell deathe0
- Direction
- suppressede0
- Value
- six genese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified six genes that, when silenced, suppressed LOV1-mediated, victorin-induced cell death”
t0“cell death in N. benthamiana”
Claim · model-written summary
Silencing the same six genes suppressed cell death induced by RPP8 expression.
- Taxon
- N. benthamianaNicotiana benthamianat0
- Treatment
- silencede0
- Gene
- RPP8e0
- Outcome
- cell death induced by expression of the closely related RPP8 resistance genee0
- Direction
- suppressede0
- Value
- six genese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified six genes that, when silenced, suppressed LOV1-mediated, victorin-induced cell death and cell death induced by expression of the closely related RPP8 resistance gene”
t0“cell death in N. benthamiana”
Claim · model-written summary
Silencing the latter four genes inhibited PTO-mediated cell death.
- Taxon
- N. benthamianaNicotiana benthamianat0
- Treatment
- Silencing the latter foure0
- Gene
- PTOe0
- Outcome
- cell deathe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing the latter four also inhibited cell death and disease resistance mediated by the PTO resistance gene.”
t0“cell death in N. benthamiana”
Claim · model-written summary
Silencing the latter four genes inhibited PTO-mediated disease resistance.
- Taxon
- N. benthamianaNicotiana benthamianat0
- Treatment
- Silencing the latter foure0
- Gene
- PTOe0
- Outcome
- disease resistancee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing the latter four also inhibited cell death and disease resistance mediated by the PTO resistance gene.”
t0“cell death in N. benthamiana”
Claim · model-written summary
The results support the interpretation that the LOV1-mediated victorin response is a defense response.
- Taxon
- N. benthamianaNicotiana benthamianat0
- Gene
- LOV1e0
- Outcome
- defense responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these results provide evidence that the victorin response mediated by LOV1 is a defense response.”
t0“cell death in N. benthamiana”
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