Abstract
One of the most severe symptoms caused by compatible plant-virus interactions is systemic necrosis, which shares common attributes with the hypersensitive response to incompatible pathogens. Although several studies have identified viral symptom determinants responsible for systemic necrosis, mechanistic models of how they contribute to necrosis in infected plants remain scarce. Here, we examined the involvement of different branches of the oxylipin biosynthesis pathway in the systemic necrosis response caused either by the synergistic interaction of Potato virus X with Potato virus Y (PVX-PVY) or by Tomato spotted wilt virus (TSWV) in Nicotiana benthamiana. Silencing either 9-lipoxygenase (LOX), 13-LOX, or α-dioxygenase-1 (α-DOX-1) attenuated the programmed cell death (PCD)-associated symptoms caused by infection with either PVX-PVY or TSWV. In contrast, silencing of the jasmonic acid perception gene, COI1 (Coronatine insensitive 1), expedited cell death during infection with compatible viruses. This correlated with an enhanced expression of oxylipin biosynthesis genes and dioxygenase activity in PVX-PVY-infected plants. Moreover, the Arabidopsis thaliana double lox1 α-dox-1 mutant became less susceptible to TSWV infection. We conclude that oxylipin metabolism is a critical component that positively regulates the process of PCD during compatible plant-virus interactions but does not play a role in restraining virus accumulation in planta.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Silencing 9-LOX, 13-LOX or α-DOX-1 attenuated the cell-death symptoms caused by PVX-PVY or TSWV infection.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae5 - Treatment
- Silencing either 9-lipoxygenase (LOX), 13-LOX, or α-dioxygenase-1 (α-DOX-1)e0
- Interactor
- PVX-PVYe0pathogen
- Second interactor
- TSWV
Orthotospovirus tomatomaculaee0pathogen - Gene
- 9-lipoxygenase (LOX), 13-LOX, or α-dioxygenase-1 (α-DOX-1)e0
- Outcome
- programmed cell death (PCD)-associated symptomse0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“in Nicotiana benthamiana”
e0“Silencing either 9-lipoxygenase (LOX), 13-LOX, or α-dioxygenase-1 (α-DOX-1) attenuated the programmed cell death (PCD)-associated symptoms caused by infection with either PVX-PVY or TSWV”
Claim · model-written summary
Silencing COI1 expedited cell death during infection with compatible viruses.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae5
- Treatment
- silencing of the jasmonic acid perception gene, COI1 (Coronatine insensitive 1)e1
- Gene
- COI1 (Coronatine insensitive 1)e1
- Outcome
- cell deathe1
- Direction
- expeditede1
- Qualifier
- during infection with compatible virusese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“in Nicotiana benthamiana”
e1“silencing of the jasmonic acid perception gene, COI1 (Coronatine insensitive 1), expedited cell death during infection with compatible viruses”
Claim · model-written summary
Enhanced expression of oxylipin biosynthesis genes and dioxygenase activity correlated with PVX-PVY infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae5
- Interactor
- PVX-PVYe2pathogen
- Outcome
- expression of oxylipin biosynthesis genes and dioxygenase activitye2
- Direction
- enhancede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“in Nicotiana benthamiana”
e2“This correlated with an enhanced expression of oxylipin biosynthesis genes and dioxygenase activity in PVX-PVY-infected plants”
Claim · model-written summary
The Arabidopsis thaliana double lox1 α-dox-1 mutant became less susceptible to TSWV infection.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae3 - Material
- double lox1 α-dox-1 mutante3
- Treatment
- TSWV infectione3
- Interactor
- TSWVOrthotospovirus tomatomaculaee3pathogen
- Outcome
- susceptible to TSWV infectione3
- Direction
- less susceptiblee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Moreover, the Arabidopsis thaliana double lox1 α-dox-1 mutant became less susceptible to TSWV infection”
Claim · model-written summary
Oxylipin metabolism does not restrain virus accumulation in planta.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae5
- Outcome
- virus accumulatione4
- Qualifier
- does not play a role in restraining virus accumulation in plantae4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“in Nicotiana benthamiana”
e4“We conclude that oxylipin metabolism is a critical component that positively regulates the process of PCD during compatible plant-virus interactions but does not play a role in restraining virus accumulation in planta”
Claim · model-written summary
Oxylipin metabolism is a critical component that positively regulates PCD during compatible plant-virus interactions.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae5
- Outcome
- the process of PCDe4
- Qualifier
- oxylipin metabolism is a critical componente4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“in Nicotiana benthamiana”
e4“We conclude that oxylipin metabolism is a critical component that positively regulates the process of PCD during compatible plant-virus interactions but does not play a role in restraining virus accumulation in planta”
The same questions in other papers
loss of function · cytology · Nicotiana benthamianaPositive and negative results
8 papers in the same direction as this paper · 24 in the opposite direction
- no effect
- negative
- negative
29 papers more in this group
See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.