Abstract
The objective of this study was to obtain transgenic tobacco (Nicotiana tabacum L. cv. Samsun NN) plants resistant to tobacco mosaic virus, TMV-vulgare strain, by the expression of a single-chain variable fragment (scFv-anti-TMV) against the TMV coat protein. The scFv-anti-TMV gene was made by combining the coding sequences of the heavy and light chain variable domains of the full antibody molecule with a sequence encoding a flexible linker. The scFv-anti-TMV was cloned under the control of the 35S promoter in the plant expression vector pBI121, and used for Agrobcterium-mediated transformation of tobacco. Integration of the scFv-anti-TMV gene into the genome of the transgenic plants and its expression were confirmed using PCR, immunoblot, and ELISA analysis. Transgenic plants expressing scFv-anti-TMV and untransformed control plants transformed with pBI121 only were inoculated with I μg/ml TMV. Constitutive expression of this scFv-anti-TMV resulted in complete immunity against TMV infection in transgenic plants (100% reduction of virus infection). In contrast, control plants were heavily infected by TMV. These results confirmed the applicability of antibody expression as a means of protecting plants from virus infection.
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Claims 2
Claim · model-written summary
Integration and expression of scFv-anti-TMV were confirmed in transgenic tobacco.
- Taxon
- tobacco
Nicotiana tabacumt0 - Gene
- scFv-anti-TMVe0
- Outcome
- Integration of the scFv-anti-TMV gene into the genome of the transgenic plants and its expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Integration of the scFv-anti-TMV gene into the genome of the transgenic plants and its expression were confirmed using PCR, immunoblot, and ELISA analysis.”
t0“in Tobacco”
Claim · model-written summary
Constitutive scFv-anti-TMV expression produced complete immunity to TMV infection, a 100% reduction relative to infected controls.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Constitutive expression of this scFv-anti-TMVe0
- Interactor
- TMV
Tobacco mosaic viruse0pathogen - Gene
- scFv-anti-TMVe0
- Outcome
- TMV infectione0
- Direction
- reductione0
- Value
- 100%e0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive expression of this scFv-anti-TMV resulted in complete immunity against TMV infection in transgenic plants (100% reduction of virus infection). In contrast, control plants were heavily infected by TMV.”
t0“in Tobacco”
The same questions in other papers
overexpression · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.