Paper · 1989 · The Plant Cell

Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection.

H J Linthorst; R L Meuwissen; S Kauffmann; et al.

4
claims
6
verbatim quotes
155
citations
3
research areas
✓
Open access

Abstract

Samsun NN tobacco cells were transformed with chimeric genes for pathogenesis-related (PR) proteins derived from genomic (PR-1a, GRP) or cDNA (PR-S) clones under the transcriptional control of the cauliflower mosaic virus 35S promoter. Regenerated plants were assayed by RNA and protein gel blotting, and plants showing high specific expression of the inserted genes were selected for self-pollination and seed formation. Inspection of second generation transformants showed that constitutive expression of PR-1a, GRP, and PR-S in tobacco in general does not have an effect on the phenotypic appearance of the plants or the expression of other endogenous PR genes. Furthermore, constitutive expression of the above genes does not affect the susceptibility of the plants to infection with tobacco mosaic virus or alfalfa mosaic virus.

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Claims 4

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Constitutive PR-1a, GRP and PR-S expression generally did not change tobacco plant appearance.

Treatment
constitutive expression of PR-1a, GRP, and PR-Se0
Gene
PR-1a, GRP, and PR-Se0
Outcome
phenotypic appearance of the plantse0
Direction
does not have an effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“constitutive expression of PR-1a, GRP, and PR-S in tobacco in general does not have an effect on the phenotypic appearance of the plants”

✓ located verbatim in the source · abstract, characters 480–616 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Constitutive PR-1a, GRP and PR-S expression generally did not change other endogenous PR gene expression in tobacco.

Treatment
constitutive expression of PR-1a, GRP, and PR-Se0
Gene
PR-1a, GRP, and PR-Se0
Outcome
expression of other endogenous PR genese0
Direction
does not have an effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“constitutive expression of PR-1a, GRP, and PR-S in tobacco in general does not have an effect on the phenotypic appearance of the plants or the expression of other endogenous PR genes”

✓ located verbatim in the source · abstract, characters 480–663 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Constitutive PR gene expression did not alter tobacco susceptibility to tobacco mosaic virus.

Treatment
constitutive expression of the above genese0
Interactor
tobacco mosaic virusTobacco mosaic viruse0pathogen
Outcome
susceptibility of the plants to infection with tobacco mosaic viruse0
Direction
does not affecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗
Tobacco mosaic virus
Tobacco mosaic virus · InteractorFrank Vincentz · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“constitutive expression of the above genes does not affect the susceptibility of the plants to infection with tobacco mosaic virus or alfalfa mosaic virus”

✓ located verbatim in the source · abstract, characters 678–832 · show in text ↑
t0

“Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection.”

✓ located verbatim in the source · title, characters 0–121 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Constitutive PR gene expression did not alter tobacco susceptibility to alfalfa mosaic virus.

Treatment
constitutive expression of the above genese0
Interactor
alfalfa mosaic virusAlfamovirus AMVe0pathogen
Outcome
susceptibility of the plants to infection with tobacco mosaic virus or alfalfa mosaic viruse0
Direction
does not affecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Nicotiana tabacum
Nicotiana tabacum · TaxonJom / Joachim Müllerchen · CC BY 2.5 · Wikimedia Commons ↗
Alfamovirus AMV
Alfamovirus AMV · InteractorHoward F. Schwartz · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“constitutive expression of the above genes does not affect the susceptibility of the plants to infection with tobacco mosaic virus or alfalfa mosaic virus”

✓ located verbatim in the source · abstract, characters 678–832 · show in text ↑
t0

“Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection.”

✓ located verbatim in the source · title, characters 0–121 · show in text ↑

overexpression · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. negative
See all 4 papers →

overexpression · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results

22 papers: 16 positive, 6 negative or no effect

5 papers in the same direction as this paper · 16 in the opposite direction

  1. positive
  2. positive
  3. positive

18 papers more in this group

See all 22 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.