Abstract
Hypocotyl explants of chilli pepper (Capsicum frutescens) were inoculated with Agrobacterium rhizogenes strain A13 harbouring the binary vector pBI121, which contains the CaMV 35S promoter linked to the GUS reporter gene. After 4 weeks of culture, the hairy roots produced were transferred onto Schenk and Hildebrandt (SH) medium supplemented with 250mgl-1 Cefotaxime. Subsequently they were selected in liquid SH medium containing 20mgl-1 kanamycin. When treated with X-glucuronide, vascular and root tip tissues were predominantly stained. A phenylalanine ammonia-lyase (PAL) cDNA from parsley (Petroselinum) was subsequently linked to the 35S promoter and transferred into hairy roots using the same A. rhizogenes strain. Successful transformation was confirmed by Southern hybridisation. Hairy roots containing the PAL transgene showed different PAL activity, slow growth and altered morphology.
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Claims 5
Claim · model-written summary
GUS staining was predominant in vascular and root tip tissues.
- Taxon
- chilli pepper (Capsicum frutescens)
Capsicum frutescense1 - Organ / stage
- vascular and root tip tissuese0
- Outcome
- predominantly stainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When treated with X-glucuronide, vascular and root tip tissues were predominantly stained.”
e1“chilli pepper (Capsicum frutescens)”
Claim · model-written summary
Southern hybridisation confirmed transformation of the hairy roots.
- Taxon
- chilli pepper (Capsicum frutescens)Capsicum frutescense1
- Gene
- PAL transgenee2
- Outcome
- Successful transformatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successful transformation was confirmed by Southern hybridisation.”
e1“chilli pepper (Capsicum frutescens)”
e2“Hairy roots containing the PAL transgene”
Claim · model-written summary
PAL transgenic hairy roots differed in PAL activity.
- Taxon
- chilli pepper (Capsicum frutescens)Capsicum frutescense1
- Gene
- PAL transgenee0
- Outcome
- PAL activitye0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hairy roots containing the PAL transgene showed different PAL activity, slow growth and altered morphology.”
e1“chilli pepper (Capsicum frutescens)”
Claim · model-written summary
PAL transgenic hairy roots grew slowly.
- Taxon
- chilli pepper (Capsicum frutescens)Capsicum frutescense1
- Gene
- PAL transgenee0
- Outcome
- growthe0
- Direction
- slowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hairy roots containing the PAL transgene showed different PAL activity, slow growth and altered morphology.”
e1“chilli pepper (Capsicum frutescens)”
Claim · model-written summary
PAL transgenic hairy roots had altered morphology.
- Taxon
- chilli pepper (Capsicum frutescens)Capsicum frutescense1
- Gene
- PAL transgenee0
- Outcome
- altered morphologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hairy roots containing the PAL transgene showed different PAL activity, slow growth and altered morphology.”
e1“chilli pepper (Capsicum frutescens)”