Abstract
Specialized transducing phages (lambda and varphi80) have been used as vectors in the transfer of genes (wild type and mutant) from the bacterium Escherichia coli to haploid cell lines of the plants Lycopersicon esculentum and Arabidopsis thaliana. The overall phenomenon of transfer, gene maintenance, transcription, translation, and function has been termed transgenosis. Transgenosis of galactose and lactose operon genes was detected by survival and growth of the plant cells on defined medium with galactose and lactose as sole sources of bulk carbon. Phages carrying a defective operon, unrelated bacterial genes, or no bacterial genes, do not affect the normal result of death on these media, nor do they prevent growth on optimal growth media. Transgenosis of the E. coli gene z (lac operon) was confirmed by a biochemical-immunological test specific for E. coli beta-galactosidase. Plant cells were unable to effectively suppress an E. coli nonsense mutation. The E. coli mutant suppressor gene, supF(+), specifies insertion of tyrosine at amber (UAG) nonsense codons. Introduction of supF(+) results in a lethal transgenosis on medium normally optimal for plant cell growth. It is concluded that amber codons are vital to the life of plant cells. Differentiating cells of A. thaliana were not affected by supF(+).
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Claims 8
Claim · model-written summary
Plant cells carrying transferred galactose operon genes survived and grew with galactose as the sole bulk carbon source.
- Taxon
- Lycopersicon esculentum and Arabidopsis thaliana
Arabidopsis thaliana
Solanum lycopersicume0 - Treatment
- Transgenosis of galactose and lactose operon genese1
- Gene
- galactose and lactose operon genese1
- Outcome
- survival and growth of the plant cellse1
- Comparator
- Phages carrying a defective operon, unrelated bacterial genes, or no bacterial genese2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid cell lines of the plants Lycopersicon esculentum and Arabidopsis thaliana”
e1“Transgenosis of galactose and lactose operon genes was detected by survival and growth of the plant cells on defined medium with galactose and lactose as sole sources of bulk carbon.”
e2“Phages carrying a defective operon, unrelated bacterial genes, or no bacterial genes, do not affect the normal result of death on these media”
Claim · model-written summary
Control phages did not prevent plant cell death on galactose or lactose medium.
- Taxon
- Lycopersicon esculentum and Arabidopsis thalianaArabidopsis thalianaSolanum lycopersicume0
- Treatment
- Phages carrying a defective operon, unrelated bacterial genes, or no bacterial genese1
- Outcome
- death on these mediae1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid cell lines of the plants Lycopersicon esculentum and Arabidopsis thaliana”
e1“Phages carrying a defective operon, unrelated bacterial genes, or no bacterial genes, do not affect the normal result of death on these media”
Claim · model-written summary
Control phages did not prevent growth of plant cells on optimal growth media.
- Taxon
- Lycopersicon esculentum and Arabidopsis thalianaArabidopsis thalianaSolanum lycopersicume0
- Outcome
- growth on optimal growth mediae1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid cell lines of the plants Lycopersicon esculentum and Arabidopsis thaliana”
e1“nor do they prevent growth on optimal growth media”
Claim · model-written summary
A biochemical immunological assay confirmed transgenosis of the E. coli z gene in plant cells.
- Taxon
- Lycopersicon esculentum and Arabidopsis thalianaArabidopsis thalianaSolanum lycopersicume0
- Gene
- E. coli gene ze1
- Outcome
- Transgenosis of the E. coli gene ze1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid cell lines of the plants Lycopersicon esculentum and Arabidopsis thaliana”
e1“Transgenosis of the E. coli gene z (lac operon) was confirmed by a biochemical-immunological test specific for E. coli beta-galactosidase”
Claim · model-written summary
Plant cells could not effectively suppress an E. coli nonsense mutation.
- Taxon
- Lycopersicon esculentum and Arabidopsis thalianaArabidopsis thalianaSolanum lycopersicume0
- Outcome
- unable to effectively suppress an E. coli nonsense mutatione1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid cell lines of the plants Lycopersicon esculentum and Arabidopsis thaliana”
e1“Plant cells were unable to effectively suppress an E. coli nonsense mutation”
Claim · model-written summary
Introduction of supF(+) was lethal to plant cells on normally optimal medium.
- Taxon
- Lycopersicon esculentum and Arabidopsis thalianaArabidopsis thalianaSolanum lycopersicume0
- Treatment
- Introduction of supF(+)e1
- Gene
- supF(+)e1
- Outcome
- lethal transgenosise1
- Comparator
- Phages carrying a defective operon, unrelated bacterial genes, or no bacterial genese2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid cell lines of the plants Lycopersicon esculentum and Arabidopsis thaliana”
e1“Introduction of supF(+) results in a lethal transgenosis on medium normally optimal for plant cell growth”
e2“Phages carrying a defective operon, unrelated bacterial genes, or no bacterial genes, do not affect the normal result of death on these media, nor do they prevent growth on optimal growth media”
Claim · model-written summary
Differentiating A. thaliana cells were unaffected by supF(+).
- Taxon
- A. thalianaArabidopsis thalianae0
- Organ / stage
- Differentiating cellse0
- Gene
- supF(+)e0
- Outcome
- not affected by supF(+)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Differentiating cells of A. thaliana were not affected by supF(+)”
Claim · model-written summary
The authors concluded that amber codons are vital to plant cell survival.
- Taxon
- Lycopersicon esculentum and Arabidopsis thalianaArabidopsis thalianaSolanum lycopersicume0
- Gene
- amber codonse1
- Outcome
- vital to the life of plant cellse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“haploid cell lines of the plants Lycopersicon esculentum and Arabidopsis thaliana”
e1“It is concluded that amber codons are vital to the life of plant cells”
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See all 36 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.