Abstract
Plants form shoot meristems in the so-called boundary region, and these meristems are necessary for normal morphogenesis of aerial parts of plants. However, the molecular mechanisms that regulate the formation of shoot meristems are not fully understood. We report here that expression of a chimeric repressor from TCP3 (TCP3SRDX), a member of TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) transcription factors in Arabidopsis thaliana, resulted in the formation of ectopic shoots on cotyledons and various defects in organ development. Expression of TCP3SRDX induced ectopic expression of boundary-specific genes, namely the CUP-SHAPED COTYLEDON (CUC) genes, and suppressed the expression of miR164, whose product cleaves the transcripts of CUC genes. This abnormal phenotype was substantially reversed on the cuc1 mutant background. By contrast, gain of function of TCP3 suppressed the expression of CUC genes and resulted in the fusion of cotyledons and defects in formation of shoots. The pattern of expression of TCP3 did not overlap with that of the CUC genes. In addition, we found that eight TCPs had functions similar to that of TCP3. Our results demonstrate that the TCP transcription factors play a pivotal role in the control of morphogenesis of shoot organs by negatively regulating the expression of boundary-specific genes.
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Claims 10
Claim · model-written summary
TCP3SRDX expression caused ectopic shoots on cotyledons.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- expression of a chimeric repressor from TCP3 (TCP3SRDX)e0
- Gene
- TCP3SRDXe0
- Outcome
- formation of ectopic shoots on cotyledonse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of a chimeric repressor from TCP3 (TCP3SRDX), a member of TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) transcription factors in Arabidopsis thaliana, resulted in the formation of ectopic shoots on cotyledons and various defects in organ development”
Claim · model-written summary
TCP3SRDX expression caused organ development defects.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- expression of a chimeric repressor from TCP3 (TCP3SRDX)e0
- Gene
- TCP3SRDXe0
- Outcome
- defects in organ developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of a chimeric repressor from TCP3 (TCP3SRDX), a member of TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) transcription factors in Arabidopsis thaliana, resulted in the formation of ectopic shoots on cotyledons and various defects in organ development”
Claim · model-written summary
TCP3SRDX induced ectopic CUC expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Expression of TCP3SRDXe0
- Gene
- CUP-SHAPED COTYLEDON (CUC) genese0
- Outcome
- expression of boundary-specific genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of TCP3SRDX induced ectopic expression of boundary-specific genes, namely the CUP-SHAPED COTYLEDON (CUC) genes, and suppressed the expression of miR164”
t0“Arabidopsis”
Claim · model-written summary
TCP3SRDX suppressed miR164 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Expression of TCP3SRDXe0
- Gene
- miR164e0
- Outcome
- expression of miR164e0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of TCP3SRDX induced ectopic expression of boundary-specific genes, namely the CUP-SHAPED COTYLEDON (CUC) genes, and suppressed the expression of miR164”
t0“Arabidopsis”
Claim · model-written summary
The cuc1 background substantially reversed the abnormal phenotype.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cuc1 mutante0
- Treatment
- cuc1 mutant backgrounde0
- Outcome
- abnormal phenotypee0
- Direction
- substantially reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This abnormal phenotype was substantially reversed on the cuc1 mutant background”
t0“Arabidopsis”
Claim · model-written summary
TCP3 gain of function suppressed CUC gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- gain of function of TCP3e0
- Gene
- CUC genese0
- Outcome
- expression of CUC genese0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gain of function of TCP3 suppressed the expression of CUC genes and resulted in the fusion of cotyledons and defects in formation of shoots”
t0“Arabidopsis”
Claim · model-written summary
TCP3 gain of function caused cotyledon fusion and shoot formation defects.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- gain of function of TCP3e0
- Outcome
- fusion of cotyledons and defects in formation of shootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gain of function of TCP3 suppressed the expression of CUC genes and resulted in the fusion of cotyledons and defects in formation of shoots”
t0“Arabidopsis”
Claim · model-written summary
TCP3 and CUC gene expression patterns did not overlap.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- TCP3e0
- Outcome
- pattern of expression of TCP3e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pattern of expression of TCP3 did not overlap with that of the CUC genes”
t0“Arabidopsis”
Claim · model-written summary
Eight TCPs had functions similar to TCP3.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- TCPse0
- Outcome
- functions similar to that of TCP3e0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eight TCPs had functions similar to that of TCP3”
t0“Arabidopsis”
Claim · model-written summary
TCP factors control shoot organ morphogenesis through negative regulation of boundary-specific genes.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- TCP transcription factorse0
- Outcome
- morphogenesis of shoot organse0
- Qualifier
- negatively regulating the expression of boundary-specific genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the TCP transcription factors play a pivotal role in the control of morphogenesis of shoot organs by negatively regulating the expression of boundary-specific genes”
t0“Arabidopsis”
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