Abstract
The Pti4, Pti5, and Pti6 proteins from tomato were identified based on their interaction with the product of the Pto disease resistance gene, a Ser-Thr protein kinase. They belong to the ethylene-response factor (ERF) family of plant-unique transcription factors and bind specifically to the GCC-box cis element present in the promoters of many pathogenesis-related (PR) genes. Here, we show that these tomato ERFs are localized to the nucleus and function in vivo as transcription activators that regulate the expression of GCC box-containing PR genes. Expression of Pti4, Pti5, or Pti6 in Arabidopsis activated the expression of the salicylic acid-regulated genes PR1 and PR2. Expression of jasmonic acid- and ethylene-regulated genes, such as PR3, PR4, PDF1.2, and Thi2.1, was affected differently by each of the three tomato ERFs, with Arabidopsis-Pti4 plants having very high levels of PDF1.2 transcripts. Exogenous application of salicylic acid to Arabidopsis-Pti4 plants suppressed the increased expression of PDF1.2 but further stimulated PR1 expression. Arabidopsis plants expressing Pti4 displayed increased resistance to the fungal pathogen Erysiphe orontii and increased tolerance to the bacterial pathogen Pseudomonas syringae pv tomato. These results indicate that Pti4, Pti5, and Pti6 activate the expression of a wide array of PR genes and play important and distinct roles in plant defense.
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Claims 8
Claim · model-written summary
Tomato Pti factors localized to nuclei and activated GCC box containing PR genes.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- tomato ERFse0
- Outcome
- expression of GCC box-containing PR genese0
- Qualifier
- localized to the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these tomato ERFs are localized to the nucleus and function in vivo as transcription activators that regulate the expression of GCC box-containing PR genes.”
t0“Arabidopsis”
Claim · model-written summary
Pti4, Pti5 or Pti6 expression activated PR1 and PR2.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Expression of Pti4, Pti5, or Pti6e0
- Gene
- Pti4, Pti5, or Pti6e0
- Outcome
- expression of the salicylic acid-regulated genes PR1 and PR2e0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of Pti4, Pti5, or Pti6 in Arabidopsis activated the expression of the salicylic acid-regulated genes PR1 and PR2.”
Claim · model-written summary
Each tomato ERF affected jasmonic acid and ethylene regulated genes differently.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- tomato ERFse0
- Outcome
- Expression of jasmonic acid- and ethylene-regulated genese0
- Direction
- affected differentlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of jasmonic acid- and ethylene-regulated genes, such as PR3, PR4, PDF1.2, and Thi2.1, was affected differently by each of the three tomato ERFs, with Arabidopsis-Pti4 plants having very high levels of PDF1.2 transcripts.”
Claim · model-written summary
Arabidopsis Pti4 plants had very high PDF1.2 transcripts.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Arabidopsis-Pti4 plantse0
- Gene
- PDF1.2e0
- Outcome
- levels of PDF1.2 transcriptse0
- Direction
- very highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis-Pti4 plants having very high levels of PDF1.2 transcripts.”
Claim · model-written summary
Salicylic acid suppressed elevated PDF1.2 expression in Pti4 plants.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Arabidopsis-Pti4 plantse0
- Treatment
- Exogenous application of salicylic acide0
- Gene
- PDF1.2e0
- Outcome
- expression of PDF1.2e0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of salicylic acid to Arabidopsis-Pti4 plants suppressed the increased expression of PDF1.2 but further stimulated PR1 expression.”
Claim · model-written summary
Salicylic acid further stimulated PR1 expression in Pti4 plants.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Arabidopsis-Pti4 plantse0
- Treatment
- Exogenous application of salicylic acide0
- Gene
- PR1e0
- Outcome
- PR1 expressione0
- Direction
- further stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of salicylic acid to Arabidopsis-Pti4 plants suppressed the increased expression of PDF1.2 but further stimulated PR1 expression.”
Claim · model-written summary
Pti4 plants had increased resistance to Erysiphe orontii.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- expressing Pti4e0
- Interactor
- Erysiphe orontii
.jpg?width=60)
Golovinomyces orontiie0pathogen - Outcome
- resistance to the fungal pathogen Erysiphe orontiie0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Arabidopsis plants expressing Pti4 displayed increased resistance to the fungal pathogen Erysiphe orontii and increased tolerance to the bacterial pathogen Pseudomonas syringae pv tomato.”
Claim · model-written summary
Pti4 plants had increased tolerance to Pseudomonas syringae pv tomato.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- expressing Pti4e0
- Interactor
- Pseudomonas syringae pv tomato
Pseudomonas syringaee0pathogen - Outcome
- tolerance to the bacterial pathogen Pseudomonas syringae pv tomatoe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis plants expressing Pti4 displayed increased resistance to the fungal pathogen Erysiphe orontii and increased tolerance to the bacterial pathogen Pseudomonas syringae pv tomato.”
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See all 46 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.