Intervention effectmodel-written summary

ICE1(K393R) expression induced MYB15 transcript accumulation.

A claim from SIZ1-Mediated Sumoylation of ICE1 ControlsCBF3/DREB1AExpression and Freezing Tolerance inArabidopsis · 2007

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Taxon: ArabidopsisArabidopsist0
Treatment: expression of ICE1(K393R)e0
Gene: MYB15e0
Outcome: transcript accumulation of MYB15e0
Direction: inducede0

Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

*Arabidopsis* · Taxon · picture of the genus (one of its species)Original uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0
“expression of ICE1(K393R) induced transcript accumulation of MYB15”
✓ located verbatim in the source · abstract, characters 1021–1087

t0
“Arabidopsis”
✓ located verbatim in the source · title, characters 89–100

Title

SIZ1-Mediated Sumoylation of ICE1 ControlsCBF3/DREB1AExpression and Freezing Tolerance inArabidopsis

Kenji Miura; Jing Bo Jin; Jiyoung Lee; et al. The Plant Cell 2007. doi:10.1105/tpc.106.048397

Other claims from this paper (9)

Intervention effect The siz1-2 and siz1-3 alleles caused freezing and chilling sensitivity, which SIZ1 expression complemented.
Intervention effect siz1 repressed cold-induced CBF/DREB1 and regulon gene expression, especially CBF3/DREB1A.
Intervention effect siz1 did not affect ICE1 expression.
Intervention effect ICE1 K393R blocked SIZ1-mediated sumoylation in vitro and in protoplasts, identifying K393 as the principal SUMO conjugation site.
Intervention effect Cold moderately induced SIZ1-dependent ICE1 sumoylation in protoplasts.
Intervention effect Sumoylation reduced polyubiquitination of recombinant ICE1 in vitro.
Intervention effect ICE1(K393R) expression repressed cold-induced CBF3/DREB1A expression in wild-type plants.
Intervention effect ICE1(K393R) expression increased freezing sensitivity in wild-type plants.
Mechanism The authors propose that SIZ1-dependent ICE1 sumoylation activates or stabilizes ICE1, enabling CBF3/DREB1A expression and MYB15 repression and leading to low-temperature tolerance.

Record rev-1b080e7a4bcb98d8573d06f51978bf2f4549f83003e6194b1028d45782046671 · schema claims-v0.9 · position 9 in the model’s answer

transgenic · molecular: expression · ArabidopsisPositive and negative results

46 papers: 20 positive, 26 negative or no effect

25 papers in the same direction as this claim · 20 in the opposite direction

positive
Increased CBF1 expression induced COR gene expression.
Arabidopsis CBF1 Overexpression Induces COR Genes and Enhances Freezing Tolerance · 1998
Opposite directionCited by this paper
positive
Pti4, Pti5 or Pti6 expression activated PR1 and PR2.
Tomato Transcription Factors Pti4, Pti5, and Pti6 Activate Defense Responses When Expressed in Arabidopsis · 2002
Opposite direction
positive
MBF1c expression augmented defense transcript accumulation under heat stress.
Enhanced Tolerance to Environmental Stress in Transgenic Plants Expressing the Transcriptional Coactivator Multiprotein Bridging Factor 1c · 2005
Opposite direction
positive
FCA intronless transgenes increased FCA protein levels in Arabidopsis.
Functional Significance of the Alternative Transcript Processing of the Arabidopsis Floral Promoter FCA · 2002
Opposite direction
positive
Shoot-specific OPT3 expression complemented aberrant IRT1 expression in opt3-2 roots.
OPT3 Is a Component of the Iron-Signaling Network between Leaves and Roots and Misregulation of OPT3 Leads to an Over-Accumulation of Cadmium in Seeds · 2014
Opposite direction
positive
Induced avrRpt2 expression also induced PR1 expression in RPS2 lines.
Glucocorticoid‐inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death · 1998
Opposite direction
positive
Induced Aplysia ADPR cyclase expression elevated KIN2, RD22, RD29a and COR47 expression less than ABA induction.
ABA activates ADPR cyclase and cADPR induces a subset of ABA‐responsive genes in Arabidopsis · 2004 · compared with ABA induction
Opposite direction
positive
Ectopic ABI3 increased ABA induction of Rab18 mRNA.
Interactions between the ABI1 and the ectopically expressed ABI3 genes in controlling abscisic acid responses in Arabidopsis vegetative tissues · 1997
Opposite direction

37 papers more in this group

See all 46 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.

Other papers on the same question

transgenic · molecular: expression · ArabidopsisPositive and negative results