Abstract
In Arabidopsis, the abscisic acid (ABA)-Insensitive ABI1 and ABI3 genes have been proposed to act in separate ABA signalling cascades. Recessive mutations in ABI3 alter various physiological processes during seed development, whereas the dominant abi1 mutation inhibits ABA responses largely in vegetative tissues. The seed-specific ABI3 gene was ectopically expressed in the vegetative tissues of transgenic Arabidopsis plants carrying a transcriptional fusion between the CaMV 35S promoter and the ABI3 cDNA. Genetic interactions between the ectopically expressed ABI3 and endogenous ABI1 genes were investigated by monitoring diverse ABA responses in vegetative tissues. Ectopic expression of ABI3 conferred to plantlets the ability to accumulate the seed-specific At2S33 and AtEm1 mRNAs in response to ABA, and the abi1 mutation inhibited this ABI3-dependent induction of AtEm1 by ABA. Furthermore, ectopic expression of ABI3 also influenced ABI1-dependent responses that occur in wild-type vegetative tissues. Expression of ABI3 increased ABA induction of the Rab18 mRNA and ABA inhibition of root growth, and both responses were sensitive to the abi1 mutation in the presence as in the absence of ABI3. Finally, although ABI3 is thought to be a transcription activator and stomatal regulation is not known to involve transcriptional events, the ectopically expressed ABI3 gene suppressed the effect of the abi1 mutation on stomatal regulation. The present data demonstrate that ABI1 and ABI3 genetically interact in controlling diverse ABA responses in transgenic vegetative tissues. The possibility that the endogenous ABI1 and ABI3 genes may similarly act in a common ABA signalling pathway in seed is discussed with previous phenotypic studies of the abi1 and abi3 mutants.
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Claims 6
Claim · model-written summary
Ectopic ABI3 enabled ABA-induced At2S33 and AtEm1 transcript accumulation.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Ectopic expression of ABI3e1
- Gene
- ABI3e1
- Outcome
- At2S33 and AtEm1 mRNAse1
- Direction
- accumulatee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, the abscisic acid (ABA)-Insensitive ABI1 and ABI3 genes”
e1“Ectopic expression of ABI3 conferred to plantlets the ability to accumulate the seed-specific At2S33 and AtEm1 mRNAs in response to ABA”
Claim · model-written summary
The abi1 mutation inhibited ABA induction of AtEm1 dependent on ectopic ABI3.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- abi1 mutatione1
- Gene
- AtEm1e1
- Outcome
- ABI3-dependent induction of AtEm1e1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, the abscisic acid (ABA)-Insensitive ABI1 and ABI3 genes”
e1“the abi1 mutation inhibited this ABI3-dependent induction of AtEm1 by ABA”
Claim · model-written summary
Ectopic ABI3 increased ABA induction of Rab18 mRNA.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Expression of ABI3e1
- Gene
- Rab18e1
- Outcome
- ABA induction of the Rab18 mRNAe1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, the abscisic acid (ABA)-Insensitive ABI1 and ABI3 genes”
e1“Expression of ABI3 increased ABA induction of the Rab18 mRNA and ABA inhibition of root growth”
Claim · model-written summary
Ectopic ABI3 increased ABA inhibition of root growth.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- roote1
- Treatment
- Expression of ABI3e1
- Outcome
- ABA inhibition of root growthe1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, the abscisic acid (ABA)-Insensitive ABI1 and ABI3 genes”
e1“Expression of ABI3 increased ABA induction of the Rab18 mRNA and ABA inhibition of root growth”
Claim · model-written summary
The abi1 mutation affected Rab18 induction and root-growth inhibition both with and without ABI3.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- abi1 mutatione1
- Outcome
- both responsese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, the abscisic acid (ABA)-Insensitive ABI1 and ABI3 genes”
e1“both responses were sensitive to the abi1 mutation in the presence as in the absence of ABI3”
Claim · model-written summary
Ectopic ABI3 suppressed the effect of abi1 on stomatal regulation.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- ectopically expressed ABI3 genee1
- Gene
- ABI3e1
- Outcome
- effect of the abi1 mutation on stomatal regulatione1
- Direction
- suppressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis, the abscisic acid (ABA)-Insensitive ABI1 and ABI3 genes”
e1“the ectopically expressed ABI3 gene suppressed the effect of the abi1 mutation on stomatal regulation”
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