Abstract
A 5-kD plant defensin was purified from Arabidopsis leaves challenged with the fungus Alternaria brassicicola and shown to possess antifungal properties in vitro. The corresponding plant defensin gene was induced after treatment of leaves with methyl jasmonate or ethylene but not with salicylic acid or 2,6-dichloroisonicotinic acid. When challenged with A. brassicicola, the levels of the plant defensin protein and mRNA rose both in inoculated leaves and in nontreated leaves of inoculated plants (systemic leaves). These events coincided with an increase in the endogenous jasmonic acid content of both types of leaves. Systemic pathogen-induced expression of the plant defensin gene was unaffected in Arabidopsis transformants (nahG) or mutants (npr1 and cpr1) affected in the salicylic acid response but was strongly reduced in the Arabidopsis mutants eln2 and col1 that are blocked in their response to ethylene and methyl jasmonate, respectively. Our results indicate that systemic pathogen-induced expression of the plant defensin gene in Arabidopsis is independent of salicylic acid but requires components of the ethylene and jasmonic acid response.
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Claims 5
Claim · model-written summary
A plant defensin purified from Arabidopsis leaves showed antifungal properties in vitro.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- leavese0
- Interactor
- Alternaria brassicicola
Alternaria brassicicolae0pathogen - Gene
- plant defensine0
- Outcome
- antifungal propertiese0
- Qualifier
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 5-kD plant defensin was purified from Arabidopsis leaves challenged with the fungus Alternaria brassicicola and shown to possess antifungal properties in vitro”
Claim · model-written summary
Methyl jasmonate or ethylene treatment induced the plant defensin gene in Arabidopsis leaves.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- leavese1
- Treatment
- methyl jasmonate or ethylenee1
- Gene
- plant defensin genee1
- Outcome
- plant defensin genee1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 5-kD plant defensin was purified from Arabidopsis leaves challenged with the fungus Alternaria brassicicola and shown to possess antifungal properties in vitro”
e1“The corresponding plant defensin gene was induced after treatment of leaves with methyl jasmonate or ethylene but not with salicylic acid or 2,6-dichloroisonicotinic acid”
Claim · model-written summary
Salicylic acid and 2,6-dichloroisonicotinic acid did not induce the plant defensin gene in Arabidopsis leaves.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- leavese1
- Treatment
- salicylic acid or 2,6-dichloroisonicotinic acide1
- Outcome
- plant defensin genee1
- Direction
- no_changeinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 5-kD plant defensin was purified from Arabidopsis leaves challenged with the fungus Alternaria brassicicola and shown to possess antifungal properties in vitro”
e1“The corresponding plant defensin gene was induced after treatment of leaves with methyl jasmonate or ethylene but not with salicylic acid or 2,6-dichloroisonicotinic acid”
Claim · model-written summary
Challenge with A. brassicicola raised defensin protein and mRNA levels in both inoculated and systemic leaves of Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- challenged with A. brassicicolae1
- Interactor
- A. brassicicolaAlternaria brassicicolae1pathogen
- Outcome
- levels of the plant defensin protein and mRNAe1
- Direction
- rosee1
- Qualifier
- both in inoculated leaves and in nontreated leaves of inoculated plantse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 5-kD plant defensin was purified from Arabidopsis leaves challenged with the fungus Alternaria brassicicola and shown to possess antifungal properties in vitro”
e1“When challenged with A. brassicicola, the levels of the plant defensin protein and mRNA rose both in inoculated leaves and in nontreated leaves of inoculated plants (systemic leaves)”
Claim · model-written summary
Pathogen challenge coincided with increased endogenous jasmonic acid content in both inoculated and systemic leaves of Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- both types of leavese1
- Outcome
- endogenous jasmonic acid contente1
- Direction
- increasee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 5-kD plant defensin was purified from Arabidopsis leaves challenged with the fungus Alternaria brassicicola and shown to possess antifungal properties in vitro”
e1“These events coincided with an increase in the endogenous jasmonic acid content of both types of leaves”
The same questions in other papers
salicylic acid · molecular: expression · ArabidopsisPositive and negative results
14 papers in the same direction as this paper · 26 in the opposite direction
- no effect
- positive
- positive
37 papers more in this group
See all 41 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.