Abstract
The onset and progression of senescence are under genetic and environmental control. The Arabidopsis thaliana NAC transcription factor ANAC092 (also called AtNAC2 and ORE1) has recently been shown to control age-dependent senescence, but its mode of action has not been analysed yet. To explore the regulatory network administered by ANAC092 we performed microarray-based expression profiling using estradiol-inducible ANAC092 overexpression lines. Approximately 46% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes, suggesting that the NAC factor exerts its role in senescence through a regulatory network that includes many of the genes previously reported to be senescence regulated. We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR. We also found that the majority of them (24 genes) are up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092, which itself is salt responsive. Furthermore, 24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with low expression at early and elevated expression at late stages of seed development. Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants. We also detected a delay of salinity-induced chlorophyll loss in detached anac092-1 mutant leaves. Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower ageing and in response to salt stress. We conclude that ANAC092 exerts its functions during senescence and seed germination through partly overlapping target gene sets.
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Claims 9
Claim · model-written summary
About 46% of 170 ANAC092-induced genes were known senescence-associated genes.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Gene
- ANAC092e0
- Outcome
- genes up-regulated upon ANAC092 induction are known senescence-associated genese0
- Value
- Approximately 46%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Approximately 46% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
Claim · model-written summary
Thirty-nine candidate genes showed a confirmed time-dependent response to ANAC092 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- ANAC092e0
- Outcome
- time-dependent response to enhanced ANAC092 expressione0
- Value
- 39e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR.”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
Claim · model-written summary
Salt stress up-regulated 24 of the candidate genes, as well as salt-responsive ANAC092.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- salt stresse0
- Gene
- ANAC092e0
- Outcome
- 24 genese0
- Direction
- up-regulatede0
- Value
- 24 genese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the majority of them (24 genes) are up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092, which itself is salt responsive”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
Claim · model-written summary
Twenty-four genes were expressed more highly at late than early seed development stages.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- seed growthe0
- Gene
- ANAC092e0
- Outcome
- expressione0
- Direction
- elevatede0
- Value
- 24 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with low expression at early and elevated expression at late stages of seed development”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
Claim · model-written summary
ANAC092 disruption increased seed germination rate under saline conditions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- Disruption of ANAC092e0
- Gene
- ANAC092e0
- Outcome
- rate of seed germinatione0
- Direction
- increasede0
- Setting
- saline conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of ANAC092 increased the rate of seed germination under saline conditions”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
Claim · model-written summary
ANAC092 overexpression had the opposite effect on seed germination under saline conditions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpression plantse0
- Gene
- ANAC092e0
- Outcome
- rate of seed germinatione0
- Direction
- decreaseinferrede0
- Setting
- saline conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
Claim · model-written summary
The anac092-1 mutation delayed salt-induced chlorophyll loss in detached leaves.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- anac092-1e0
- Organ / stage
- detached anac092-1 mutant leavese0
- Treatment
- salinitye0
- Outcome
- chlorophyll losse0
- Direction
- delaye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a delay of salinity-induced chlorophyll loss in detached anac092-1 mutant leaves”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
Claim · model-written summary
ANAC092 transcription was controlled during leaf and flower ageing and by salt stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- leaf and flower ageinge0
- Gene
- ANAC092e0
- Outcome
- transcriptional control of ANAC092 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower ageing and in response to salt stress.”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
Claim · model-written summary
The authors infer that partly overlapping ANAC092 target sets act during senescence and germination.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- ANAC092e0
- Outcome
- functions during senescence and seed germinatione0
- Qualifier
- partly overlapping target gene setse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ANAC092 exerts its functions during senescence and seed germination through partly overlapping target gene sets”
e1“The Arabidopsis thaliana NAC transcription factor ANAC092”
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