Abstract
Green barley leaves (Hordeum vulgaris) floated on the surface of 0.05 m ethylenediamine disodium tetraacetate, EDTA-2Na, pH 7.0 and exposed to light (5000 lux) at 25 degrees exhibited a marked bleaching (EDTA-bleaching) visible to the naked eye and paralleled by a striking reduction in content of chlorophylls a and b. This loss of color did not occur in controls which were treated with H(2)O instead of EDTA (water controls). In darkness the leaves in the water controls were bleached while EDTA-treated leaves retained all their color.EDTA bleaching was observed only in intact leaves. When leaves were boiled EDTA protected their pigment against photodecomposition. Without EDTA boiled leaves were bleached completely in light. When intact green leaves which had been floated on water and exposed to light for 48 hr were treated with boiling ethanol or acetone, the chlorophylls extracted by this procedure did not undergo bleaching if EDTA were present in solution. Under these conditions a green fine grain precipitate formed which was insoluble in ethanol or acetone and was stable in light or darkness.EDTA bleaching of green barley leaves was inhibited by KCN, and by the addition of casein hydrolysate.
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Claims 12
Claim · model-written summary
Light-exposed EDTA treated leaves bleached markedly.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- EDTA-2Nae0
- Outcome
- bleachinge0
- Direction
- markede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Green barley leaves (Hordeum vulgaris) floated on the surface of 0.05 m ethylenediamine disodium tetraacetate, EDTA-2Na, pH 7.0 and exposed to light (5000 lux) at 25 degrees exhibited a marked bleaching”
t0“barley”
Claim · model-written summary
Chlorophyll a and b content fell with EDTA bleaching.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- content of chlorophylls a and be0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“paralleled by a striking reduction in content of chlorophylls a and b”
t0“barley”
Claim · model-written summary
Light-exposed water controls did not lose color.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- H(2)Oe0
- Outcome
- loss of colore0
- Direction
- did not occure0
- Comparator
- EDTAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This loss of color did not occur in controls which were treated with H(2)O instead of EDTA (water controls)”
t0“barley”
Claim · model-written summary
In darkness water controls bleached.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- bleachede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In darkness the leaves in the water controls were bleached”
t0“barley”
Claim · model-written summary
In darkness EDTA treated leaves retained their color.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- retained all their colore0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EDTA-treated leaves retained all their color”
t0“barley”
Claim · model-written summary
EDTA bleaching occurred only in intact leaves.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- EDTA bleachinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EDTA bleaching was observed only in intact leaves”
t0“barley”
Claim · model-written summary
EDTA protected pigment of boiled leaves from photodecomposition.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- EDTAe0
- Outcome
- photodecompositione0
- Direction
- protectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When leaves were boiled EDTA protected their pigment against photodecomposition”
t0“barley”
Claim · model-written summary
Boiled leaves without EDTA bleached completely in light.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Without EDTAe0
- Outcome
- bleachede0
- Direction
- completelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Without EDTA boiled leaves were bleached completely in light”
t0“barley”
Claim · model-written summary
Extracted chlorophyll did not bleach when EDTA was present.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- EDTAe0
- Outcome
- bleachinge0
- Direction
- did not undergoe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the chlorophylls extracted by this procedure did not undergo bleaching if EDTA were present in solution”
t0“barley”
Claim · model-written summary
A green precipitate formed that resisted solvents and light or darkness.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- green fine grain precipitatee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a green fine grain precipitate formed which was insoluble in ethanol or acetone and was stable in light or darkness”
t0“barley”
Claim · model-written summary
KCN inhibited EDTA bleaching.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- KCNe0
- Outcome
- EDTA bleachinge0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EDTA bleaching of green barley leaves was inhibited by KCN”
t0“barley”
Claim · model-written summary
Casein hydrolysate inhibited EDTA bleaching.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- casein hydrolysatee0
- Outcome
- EDTA bleachinge0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EDTA bleaching of green barley leaves was inhibited by KCN, and by the addition of casein hydrolysate”
t0“barley”