Abstract
Upon posttranslational activation, the eukaryotic initiation factor-5A (eIF-5A) transports a subset of mRNAs out of the nucleus to the ribosomes for translation. Activation of the protein is an evolutionary highly conserved process that is unique to eIF-5A, the conversion of a lysine to a hypusine. Instrumental for the synthesis of hypusine is the first of two enzymatic reactions mediated by deoxyhypusine synthase (DHS). We show that DHS of wheat and the pathogenic fungus Fusarium graminearum, which causes one of the most destructive crop diseases worldwide, are transcriptionally upregulated during their pathogenic interaction. Although DHS of wheat, fungus, and human can be equally inhibited by the inhibitor CNI-1493 in vitro, application during infection of wheat and maize flowers results in strong inhibition of the pathogen without interference with kernel development. Our studies provide a novel strategy to selectively inhibit fungal growth without affecting plant growth. We identified fungal DHS as a target for the development of new inhibitors, for which CNI-1493 may serve as a lead substance.
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Claims 8
Claim · model-written summary
Wheat DHS was transcriptionally upregulated during fungal infection.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Gene
- DHSe0
- Outcome
- transcriptionally upregulatede0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“DHS of wheat and the pathogenic fungus Fusarium graminearum, which causes one of the most destructive crop diseases worldwide, are transcriptionally upregulated during their pathogenic interaction”
Claim · model-written summary
Fusarium DHS was transcriptionally upregulated during interaction with wheat.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Fusarium graminearumFusarium graminearume0pathogen
- Gene
- DHSe0
- Outcome
- DHS of wheat and the pathogenic fungus Fusarium graminearume0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DHS of wheat and the pathogenic fungus Fusarium graminearum, which causes one of the most destructive crop diseases worldwide, are transcriptionally upregulated during their pathogenic interaction”
Claim · model-written summary
CNI-1493 inhibited wheat, fungal and human DHS equally in vitro.
- Taxon
- wheatTriticum aestivume0
- Treatment
- CNI-1493e0
- Gene
- DHSe0
- Outcome
- DHS of wheate0
- Direction
- inhibitede0
- Comparator
- fungus, and humane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DHS of wheat, fungus, and human can be equally inhibited by the inhibitor CNI-1493 in vitro”
Claim · model-written summary
CNI-1493 strongly inhibited the pathogen during wheat flower infection.
- Taxon
- wheatTriticum aestivume0
- Treatment
- CNI-1493e1
- Interactor
- pathogene0pathogen
- Outcome
- inhibition of the pathogene0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application during infection of wheat and maize flowers results in strong inhibition of the pathogen without interference with kernel development”
e1“the inhibitor CNI-1493 in vitro”
Claim · model-written summary
CNI-1493 strongly inhibited the pathogen during maize flower infection.
- Treatment
- CNI-1493e1
- Interactor
- pathogene0pathogen
- Outcome
- inhibition of the pathogene0
- Direction
- inhibitione0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application during infection of wheat and maize flowers results in strong inhibition of the pathogen without interference with kernel development”
e1“the inhibitor CNI-1493 in vitro”
Claim · model-written summary
CNI-1493 did not interfere with wheat kernel development.
- Taxon
- wheatTriticum aestivume0
- Treatment
- CNI-1493e1
- Outcome
- kernel developmente0
- Direction
- without interferencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application during infection of wheat and maize flowers results in strong inhibition of the pathogen without interference with kernel development”
e1“the inhibitor CNI-1493 in vitro”
Claim · model-written summary
CNI-1493 did not interfere with maize kernel development.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application during infection of wheat and maize flowers results in strong inhibition of the pathogen without interference with kernel development”
e1“the inhibitor CNI-1493 in vitro”
Claim · model-written summary
The authors identified fungal DHS as a target for new inhibitors and CNI-1493 as a possible lead.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- fungale0pathogen
- Outcome
- fungal DHS as a target for the development of new inhibitorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified fungal DHS as a target for the development of new inhibitors, for which CNI-1493 may serve as a lead substance”
t0“Wheat and Cob Rot of Maize”