Abstract
ABSTRACT Optimum conditions for the extraction and purification of avocado cellulase have been established. The best quantiative recovery of the cellulase from avocado tissue was obtained with a 0.04M acetate buffer, pH 5.0, containing 0.4M NaCl and 0.25% (v/v) Triton X‐100. The isoelectric point of the enzyme was 4.7 and the molecular weight, based on SDS gel electrophoresis, was 49,000. The enzyme was purified on a cellulose column to a single protein band on SDS gel electrophoresis. Rabbit antibody produced to the cellulase gave further proof of the enzyme's purity.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
A specified acetate buffer gave the best quantitative recovery of avocado cellulase.
- Taxon
- avocado
Persea americanae0 - Treatment
- 0.04M acetate buffer, pH 5.0, containing 0.4M NaCl and 0.25% (v/v) Triton X‐100e0
- Gene
- cellulasee0
- Outcome
- quantiative recoverye0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“AVOCADO”
e0“The best quantiative recovery of the cellulase from avocado tissue was obtained with a 0.04M acetate buffer, pH 5.0, containing 0.4M NaCl and 0.25% (v/v) Triton X‐100”
Claim · model-written summary
Avocado cellulase had an isoelectric point of 4.7.
- Taxon
- AVOCADOPersea americanat0
- Gene
- CELLULASEt0
- Outcome
- isoelectric pointe0
- Value
- 4.7e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“AVOCADO CELLULASE”
e0“The isoelectric point of the enzyme was 4.7”
Claim · model-written summary
Avocado cellulase had a molecular weight of 49,000 by SDS gel electrophoresis.
- Taxon
- AVOCADOPersea americanat0
- Gene
- CELLULASEt0
- Outcome
- molecular weighte0
- Value
- 49,000e0
- Qualifier
- based on SDS gel electrophoresise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“AVOCADO CELLULASE”
e0“the molecular weight, based on SDS gel electrophoresis, was 49,000”
Claim · model-written summary
Cellulose column purification yielded a single protein band for avocado cellulase.
- Taxon
- AVOCADOPersea americanat0
- Treatment
- cellulose columne0
- Gene
- CELLULASEt0
- Outcome
- single protein bande0
- Value
- single protein bande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“AVOCADO CELLULASE”
e0“The enzyme was purified on a cellulose column to a single protein band on SDS gel electrophoresis”
Claim · model-written summary
Rabbit antibody provided further evidence of avocado cellulase purity.
- Taxon
- AVOCADOPersea americanat0
- Treatment
- Rabbit antibodye0
- Gene
- CELLULASEt0
- Outcome
- enzyme's puritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“AVOCADO CELLULASE”
e0“Rabbit antibody produced to the cellulase gave further proof of the enzyme's purity”