Abstract
Virus elimination in potato through meristem culture followed by thermotherapy and virus indexing was studied. Three levels of thermotherapy, viz. 27±1°C (control), 30±1°C and 35±1°C, sixteen combinations of BAP (Benzyl Amino Purine) plus GA3 (gibberellic Acid) concentrations viz. 0.0+0.0 (control), 0.0+0.2, 0.0+0.4, 0.0+0.6, 1.5+0.0, 1.5+0.2, 1.5+0.4, 1.5+0.6, 3.0+0.0, 3.0+0.2, 3.0+0.4, 3.0+0.6 , 4.5+0.0, 4.5+ 0.2, 4.5+0.4 and 4.5+0.6 were used in this study in three potato varieties viz. Diamant, Heera and Lalpakri. Among the thermo therapies, 27±1°C showed the highest (24.55) survival response followed by 30±1°C, 35±1°C, respectively. The poorest (20.47) survival response of meristem derived plantlets was noticed in 35±1°C which gave the highest percentage (43.79) of virus free plantlets followed by 30±1°C. The best (25.85%) survival response was found in Lalpakri and the lowest (19.08%) survivality was recorded in Diamant. The highest (33.27) percentage of PVY (Potato Virus Y) free plantlets was observed in Heera. The combined treatment 3.0 mg L-1 BAP and 0.2 mg L-1 GA3 showed the highest (63.39) percentage of virus free plantlet production followed by 4.5 mg L-1 BAP and 0.2 mg L-1 GA3DOI: http://dx.doi.org/10.3329/sja.v11i1.18376 SAARC J. Agri., 11(1): 71-80 (2013)
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Claims 7
Claim · model-written summary
At 27±1°C, potato meristem plantlets had the highest survival response of 24.55.
- Taxon
- potato
Solanum tuberosumt0 - Treatment
- 27±1°Ce0
- Outcome
- survival responsee0
- Direction
- higheste0
- Value
- 24.55e0
- Comparator
- 30±1°C, 35±1°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“27±1°C showed the highest (24.55) survival response followed by 30±1°C, 35±1°C, respectively”
t0“potato”
Claim · model-written summary
At 35±1°C, potato meristem plantlets had the poorest survival response of 20.47.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- meristem derived plantletse0
- Treatment
- 35±1°Ce0
- Outcome
- survival responsee0
- Direction
- pooreste0
- Value
- 20.47e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The poorest (20.47) survival response of meristem derived plantlets was noticed in 35±1°C”
t0“potato”
Claim · model-written summary
At 35±1°C, 43.79% of potato plantlets were virus free, the highest proportion.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- 35±1°Ce0
- Outcome
- virus free plantletse0
- Direction
- higheste0
- Value
- 43.79e0
- Comparator
- 30±1°Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“35±1°C which gave the highest percentage (43.79) of virus free plantlets followed by 30±1°C”
t0“potato”
Claim · model-written summary
Lalpakri had the best potato plantlet survival response, 25.85%.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Lalpakrie0
- Outcome
- survival responsee0
- Direction
- beste0
- Value
- 25.85%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best (25.85%) survival response was found in Lalpakri”
t0“potato”
Claim · model-written summary
Diamant had the lowest potato plantlet survival, 19.08%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lowest (19.08%) survivality was recorded in Diamant”
t0“potato”
Claim · model-written summary
Heera had the highest PVY-free plantlet percentage, 33.27.
- Taxon
- potatoSolanum tuberosumt0
- Material
- Heerae0
- Outcome
- PVY (Potato Virus Y) free plantletse0
- Direction
- higheste0
- Value
- 33.27e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest (33.27) percentage of PVY (Potato Virus Y) free plantlets was observed in Heera”
t0“potato”
Claim · model-written summary
The 3.0 mg L-1 BAP plus 0.2 mg L-1 GA3 treatment gave the highest virus-free plantlet production, 63.39%.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- 3.0 mg L-1 BAP and 0.2 mg L-1 GA3e0
- Outcome
- virus free plantlet productione0
- Direction
- higheste0
- Value
- 63.39e0
- Comparator
- 4.5 mg L-1 BAP and 0.2 mg L-1 GA3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combined treatment 3.0 mg L-1 BAP and 0.2 mg L-1 GA3 showed the highest (63.39) percentage of virus free plantlet production followed by 4.5 mg L-1 BAP and 0.2 mg L-1 GA3”
t0“potato”
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