Abstract
Potato (Solanum tuberosum L.) tuberization is a practically important natural process regulated by various factors including phytohormones. This work was aimed at studying characteristics of in vitro cultivated potato plants transformed with the AtGA20-oxidase gene promoting biosynthesis of bioactive gibberellins (GAs) or with the AtGA2-oxidase gene acting oppositely, i.e. deactivating functional GAs. Both transgenes originated from Arabidopsis and were fused to a sugar-sensitive B33 patatin promoter providing their expression predominantly in tubers. Global phytohormone determination in AtGA20ox-transformants revealed active GA1 at high and moderate concentrations in tubers and shoots, respectively. In control plants, GA1 was virtually absent. Together with GAs, contents of some other phytohormones were altered in transgenic plants. This was especially true for the auxin content which increased ~15-fold in tubers and more than 4-fold in shoots. Also the jasmonic acid content exhibited a tuber-specific increase while the content of abscisic acid decreased both in tubers and shoots. The dynamics of tuberization in transformed and non-transformed potato plants was recorded in in vitro parallel assays. The transgene for GA inactivation enhanced tuber formation while the transgene promoting active GA synthesis reduced potato productivity. Hence, the crucial role of gibberellin in potato productivity was corroborated. These results showed that the manipulation of the local GA level by using the foreign GA oxidase genes and organ-specific promoters is useful not only to investigate the molecular mechanisms governing tuberization, but also as a biotechnological tool for the manipulation of tuber formation without marked impairment of other physiological traits of potatoes.
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Claims 7
Claim · model-written summary
AtGA20ox potato transformants had high GA1 in tubers and moderate GA1 in shoots, whereas controls had virtually none.
- Taxon
- Potato
Solanum tuberosumt0 - Organ / stage
- tubers and shootse0
- Treatment
- AtGA20ox-transformantse0
- Gene
- AtGA20oxe0
- Outcome
- active GA1e0
- Comparator
- control plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Global phytohormone determination in AtGA20ox-transformants revealed active GA1 at high and moderate concentrations in tubers and shoots, respectively. In control plants, GA1 was virtually absent.”
t0“Potato Tuber Formation”
Claim · model-written summary
Auxin content increased about 15-fold in tubers of transgenic potatoes.
- Taxon
- PotatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Outcome
- auxin contente0
- Direction
- increasede0
- Value
- ~15-folde0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the auxin content which increased ~15-fold in tubers and more than 4-fold in shoots”
t0“Potato Tuber Formation”
Claim · model-written summary
Auxin content increased more than fourfold in shoots of transgenic potatoes.
- Taxon
- PotatoSolanum tuberosumt0
- Organ / stage
- shootse0
- Outcome
- auxin contente0
- Direction
- increasede0
- Value
- more than 4-folde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the auxin content which increased ~15-fold in tubers and more than 4-fold in shoots”
t0“Potato Tuber Formation”
Claim · model-written summary
Jasmonic acid content increased specifically in potato tubers.
- Taxon
- PotatoSolanum tuberosumt0
- Organ / stage
- tubere0
- Outcome
- jasmonic acid contente0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the jasmonic acid content exhibited a tuber-specific increase”
t0“Potato Tuber Formation”
Claim · model-written summary
Abscisic acid content decreased in potato tubers and shoots.
- Taxon
- PotatoSolanum tuberosumt0
- Organ / stage
- tubers and shootse0
- Outcome
- content of abscisic acide0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of abscisic acid decreased both in tubers and shoots”
t0“Potato Tuber Formation”
Claim · model-written summary
The GA-inactivating transgene enhanced potato tuber formation.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- transgene for GA inactivatione0
- Outcome
- tuber formatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgene for GA inactivation enhanced tuber formation while the transgene promoting active GA synthesis reduced potato productivity.”
t0“Potato Tuber Formation”
Claim · model-written summary
The transgene promoting active GA synthesis reduced potato productivity.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- transgene promoting active GA synthesise0
- Outcome
- potato productivitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgene for GA inactivation enhanced tuber formation while the transgene promoting active GA synthesis reduced potato productivity.”
t0“Potato Tuber Formation”
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Gibberellic acid · yield · Solanum tuberosum (potato)Results differ
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- positive
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.