Abstract
Streptomyces scabies causes potato common scab disease, which reduces the quality and market value of affected tubers. The predominant pathogenicity determinant produced by S. scabies is the thaxtomin A phytotoxin, which is essential for common scab disease development. Production of thaxtomin A involves the nonribosomal peptide synthetases (NRPSs) TxtA and TxtB, both of which contain an adenylation (A-) domain for selecting and activating the appropriate amino acid during thaxtomin biosynthesis. The genome of S. scabies 87.22 contains three small MbtH-like protein (MLP)-coding genes, one of which (txtH) is present in the thaxtomin biosynthesis gene cluster. MLP family members are typically required for the proper folding of NRPS A-domains and/or stimulating their activities. This study investigated the importance of TxtH during thaxtomin biosynthesis in S. scabies. Biochemical studies showed that TxtH is required for promoting the soluble expression of both the TxtA and TxtB A-domains in Escherichia coli, and amino acid residues essential for this activity were identified. Deletion of txtH in S. scabies significantly reduced thaxtomin A production, and deletion of one of the two additional MLP homologues in S. scabies completely abolished production. Engineered expression of all three S. scabies MLPs could restore thaxtomin A production in a triple MLP-deficient strain, while engineered expression of MLPs from other Streptomyces spp. could not. Furthermore, the constructed MLP mutants were reduced in virulence compared to wild-type S. scabies. The results of our study confirm that TxtH plays a key role in thaxtomin A biosynthesis and plant pathogenicity in S. scabies.
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Claims 8
Claim · model-written summary
TxtH is required for soluble expression of the TxtA and TxtB A-domains in Escherichia coli.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- TxtHe1
- Outcome
- soluble expression of both the TxtA and TxtB A-domainse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Biochemical studies showed that TxtH is required for promoting the soluble expression of both the TxtA and TxtB A-domains in Escherichia coli”
t0“potato common scab pathogen Streptomyces scabies”
Claim · model-written summary
Amino acid residues essential for the TxtH activity on A-domain solubility were identified.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- amino acid residues essential for this activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“amino acid residues essential for this activity were identified”
t0“potato common scab pathogen Streptomyces scabies”
Claim · model-written summary
Deletion of txtH in S. scabies significantly reduced thaxtomin A production.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Deletion of txtHe1
- Interactor
- S. scabiesStreptomyces scabieie1pathogen
- Outcome
- thaxtomin A productione1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of txtH in S. scabies significantly reduced thaxtomin A production”
t0“potato common scab pathogen Streptomyces scabies”
Claim · model-written summary
Deletion of one of the two additional MLP homologues in S. scabies completely abolished thaxtomin A production.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- deletion of one of the two additional MLP homologuese1
- Interactor
- S. scabiesStreptomyces scabieie1pathogen
- Outcome
- productione1
- Direction
- abolishede1
- Qualifier
- completelye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“deletion of one of the two additional MLP homologues in S. scabies completely abolished production”
t0“potato common scab pathogen Streptomyces scabies”
Claim · model-written summary
Engineered expression of all three S. scabies MLPs restored thaxtomin A production in a triple MLP-deficient strain.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Engineered expression of all three S. scabies MLPse1
- Interactor
- S. scabiesStreptomyces scabieie1pathogen
- Outcome
- thaxtomin A productione1
- Direction
- could restoree1
- Comparator
- triple MLP-deficient straine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Engineered expression of all three S. scabies MLPs could restore thaxtomin A production in a triple MLP-deficient strain”
t0“potato common scab pathogen Streptomyces scabies”
Claim · model-written summary
Engineered expression of MLPs from other Streptomyces spp. did not restore thaxtomin A production in the triple MLP-deficient strain.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- engineered expression of MLPs from other Streptomyces spp.e1
- Outcome
- thaxtomin A productione1
- Direction
- could note1
- Comparator
- triple MLP-deficient straine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Engineered expression of all three S. scabies MLPs could restore thaxtomin A production in a triple MLP-deficient strain, while engineered expression of MLPs from other Streptomyces spp. could not”
t0“potato common scab pathogen Streptomyces scabies”
Claim · model-written summary
The constructed MLP mutants were reduced in virulence compared with wild-type S. scabies.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- constructed MLP mutantse1
- Interactor
- S. scabiesStreptomyces scabieie1pathogen
- Outcome
- virulencee1
- Direction
- reducede1
- Comparator
- wild-type S. scabiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the constructed MLP mutants were reduced in virulence compared to wild-type S. scabies”
t0“potato common scab pathogen Streptomyces scabies”
Claim · model-written summary
TxtH plays a key role in thaxtomin A biosynthesis and plant pathogenicity of S. scabies.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- S. scabiesStreptomyces scabieie1pathogen
- Gene
- TxtHe1
- Outcome
- thaxtomin A biosynthesis and plant pathogenicitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The results of our study confirm that TxtH plays a key role in thaxtomin A biosynthesis and plant pathogenicity in S. scabies”
t0“potato common scab pathogen Streptomyces scabies”
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14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.