Abstract
BACKGROUND: In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR). RESULTS: Transgenic plants over-expressing dihydroflavonol reductase (DFR) were subsequently transformed with the cDNA coding for the glycosyltransferase (UGT) of Solanum sogarandinum in order to obtain plants with a high anthocyanin content without reducing tuber yield and quality. Based on enzyme studies, the recombinant UGT is a 7-O-glycosyltransferase whose natural substrates include both anthocyanidins and flavonols such as kaempferol and quercetin. In the super-transformed plants, tuber production was much higher than in the original transgenic plants bearing only the transgene coding for DFR, and was almost the same as in the control plants. The anthocyanin level was lower than in the initial plants, but still higher than in the control plants. Unexpectedly, the super-transformed plants also produced large amounts of kaempferol, chlorogenic acid, isochlorogenic acid, sinapic acid and proanthocyanins. CONCLUSION: In plants over-expressing both the transgene for DFR and the transgene for UGT, the synthesis of phenolic acids was diverted away from the anthocyanin branch. This represents a novel approach to manipulating phenolic acids synthesis in plants.
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Claims 8
Claim · model-written summary
The recombinant UGT is a 7-O-glycosyltransferase whose natural substrates include anthocyanidins and flavonols such as kaempferol and quercetin.
- Taxon
- potato plants
Solanum tuberosume0 - Gene
- UGTe1
- Compound
- kaempferol and quercetine1
- Outcome
- natural substratese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway”
e1“the recombinant UGT is a 7-O-glycosyltransferase whose natural substrates include both anthocyanidins and flavonols such as kaempferol and quercetin”
Claim · model-written summary
Super-transformed potato plants (DFR plus UGT) had much higher tuber production than the original transgenic plants carrying only DFR.
- Taxon
- potato plantsSolanum tuberosume0
- Treatment
- transformed with the cDNA coding for the glycosyltransferase (UGT)e2
- Outcome
- tuber productione3
- Direction
- much highere3
- Comparator
- the original transgenic plants bearing only the transgene coding for DFRe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway”
e2“Transgenic plants over-expressing dihydroflavonol reductase (DFR) were subsequently transformed with the cDNA coding for the glycosyltransferase (UGT) of Solanum sogarandinum”
e3“In the super-transformed plants, tuber production was much higher than in the original transgenic plants bearing only the transgene coding for DFR, and was almost the same as in the control plants”
Claim · model-written summary
Super-transformed potato plants had tuber production almost the same as control plants.
- Taxon
- potato plantsSolanum tuberosume0
- Treatment
- transformed with the cDNA coding for the glycosyltransferase (UGT)e2
- Outcome
- tuber productione3
- Direction
- almost the samee3
- Comparator
- control plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway”
e2“Transgenic plants over-expressing dihydroflavonol reductase (DFR) were subsequently transformed with the cDNA coding for the glycosyltransferase (UGT) of Solanum sogarandinum”
e3“In the super-transformed plants, tuber production was much higher than in the original transgenic plants bearing only the transgene coding for DFR, and was almost the same as in the control plants”
Claim · model-written summary
Anthocyanin level in the super-transformed plants was lower than in the initial DFR plants.
- Taxon
- potato plantsSolanum tuberosume0
- Treatment
- transformed with the cDNA coding for the glycosyltransferase (UGT)e2
- Outcome
- anthocyanin levele4
- Direction
- lowere4
- Comparator
- initial plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway”
e2“Transgenic plants over-expressing dihydroflavonol reductase (DFR) were subsequently transformed with the cDNA coding for the glycosyltransferase (UGT) of Solanum sogarandinum”
e4“The anthocyanin level was lower than in the initial plants, but still higher than in the control plants”
Claim · model-written summary
Anthocyanin level in the super-transformed plants was still higher than in control plants.
- Taxon
- potato plantsSolanum tuberosume0
- Treatment
- transformed with the cDNA coding for the glycosyltransferase (UGT)e2
- Outcome
- anthocyanin levele4
- Direction
- still highere4
- Comparator
- control plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway”
e2“Transgenic plants over-expressing dihydroflavonol reductase (DFR) were subsequently transformed with the cDNA coding for the glycosyltransferase (UGT) of Solanum sogarandinum”
e4“The anthocyanin level was lower than in the initial plants, but still higher than in the control plants”
Claim · model-written summary
Super-transformed potato plants produced large amounts of kaempferol, chlorogenic acid and isochlorogenic acid.
- Taxon
- potato plantsSolanum tuberosume0
- Outcome
- large amounts of kaempferol, chlorogenic acid, isochlorogenic acide5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway”
e5“Unexpectedly, the super-transformed plants also produced large amounts of kaempferol, chlorogenic acid, isochlorogenic acid, sinapic acid and proanthocyanins”
Claim · model-written summary
Super-transformed potato plants produced large amounts of sinapic acid and proanthocyanins.
- Taxon
- potato plantsSolanum tuberosume0
- Outcome
- sinapic acid and proanthocyaninse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway”
e5“Unexpectedly, the super-transformed plants also produced large amounts of kaempferol, chlorogenic acid, isochlorogenic acid, sinapic acid and proanthocyanins”
Claim · model-written summary
Over-expression of both DFR and UGT diverted the synthesis of phenolic acids away from the anthocyanin branch in potato.
- Taxon
- potato plantsSolanum tuberosume0
- Treatment
- over-expressing both the transgene for DFR and the transgene for UGTe6
- Outcome
- synthesis of phenolic acidse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway”
e6“In plants over-expressing both the transgene for DFR and the transgene for UGT, the synthesis of phenolic acids was diverted away from the anthocyanin branch”
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transgenic · yield · Solanum tuberosum (potato)Positive and negative results
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- positive
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See all 18 papers →transgenic · quality: phytochemicals · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.