Abstract
The present study demonstrated the use of various PGR combinations for efficient in vitro regeneration of cv. kufri jyoti in kumaun hills. Best callus induction and proliferation was observed in MS medium supplemented with 13.59 µM 2,4-D alone and 2,4-D + kinetin (9.06 + 1.16 µM) out of different concentrations of 2,4-D (4.53 to 18.12 µM) alone and 2,4-D (0 to 18.12 µM) with kinetin (1.16 µM). Leaf explants were more efficient in producing callus as compared to internodes. Medium supplemented with BA + GA3 (8.88 µM + 1 µM) initiated shoot induction out of various combinations of BA (4.44 to 13.22 µM) and GA3 (1 µM) after 7 days of incubation with significantly high average number of shoots, average shoot length and average number of leaves per explant. MS medium supplemented with different concentrations of zeatin (4.56, 9.12 and 13.68 µM) with IAA (5.71 µM) and GA3 (8.49 µM) was tried for direct regeneration of shoots through nodes out of which zeatin + IAA + GA3 (13.68 µM + 5.71 µM + 8.49 µM) served to be the best combination and the raised plantlets were found to produce microtubers in a period of 8 to 10 weeks. 2.45 µM IBA in full strength basal MS medium induced highest number of roots. In addition to an efficient regeneration protocol, the microtuber production was also studied in the present piece of work. The research protocol may also be utilized for Agrobacterium tumefaciens mediated transformation towards the biotic and abiotic stress tolerant potato crop. Key words: In vitro, potato, callus, direct regeneration, microtubers.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Two media combinations gave the best callus induction and proliferation.
- Taxon
- potato (Solanum tuberosum L.)
Solanum tuberosumt0 - Treatment
- 13.59 µM 2,4-D alone and 2,4-D + kinetin (9.06 + 1.16 µM)e0
- Outcome
- callus induction and proliferatione0
- Direction
- Beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Best callus induction and proliferation was observed in MS medium supplemented with 13.59 µM 2,4-D alone and 2,4-D + kinetin (9.06 + 1.16 µM)”
t0“potato (Solanum tuberosum L.)”
Claim · model-written summary
Leaf explants produced callus more efficiently than internodes.
- Taxon
- potato (Solanum tuberosum L.)Solanum tuberosumt0
- Organ / stage
- Leaf explantse0
- Outcome
- producing calluse0
- Direction
- more efficiente0
- Comparator
- internodese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf explants were more efficient in producing callus as compared to internodes”
t0“potato (Solanum tuberosum L.)”
Claim · model-written summary
BA plus GA3 medium initiated shoots after seven days with high shoot number, length and leaf number.
- Taxon
- potato (Solanum tuberosum L.)Solanum tuberosumt0
- Treatment
- BA + GA3 (8.88 µM + 1 µM)e0
- Outcome
- average number of shoots, average shoot length and average number of leaves per explante0
- Direction
- highe0
- Qualifier
- after 7 days of incubatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Medium supplemented with BA + GA3 (8.88 µM + 1 µM) initiated shoot induction out of various combinations of BA (4.44 to 13.22 µM) and GA3 (1 µM) after 7 days of incubation with significantly high average number of shoots, average shoot length and average number of leaves per explant”
t0“potato (Solanum tuberosum L.)”
Claim · model-written summary
Zeatin, IAA and GA3 at 13.68, 5.71 and 8.49 µM was the best direct shoot regeneration combination.
- Taxon
- potato (Solanum tuberosum L.)Solanum tuberosumt0
- Treatment
- zeatin + IAA + GA3 (13.68 µM + 5.71 µM + 8.49 µM)e0
- Outcome
- best combinatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“zeatin + IAA + GA3 (13.68 µM + 5.71 µM + 8.49 µM) served to be the best combination”
t0“potato (Solanum tuberosum L.)”
Claim · model-written summary
Regenerated plantlets produced microtubers within 8 to 10 weeks.
- Taxon
- potato (Solanum tuberosum L.)Solanum tuberosumt0
- Outcome
- produce microtuberse0
- Value
- 8 to 10 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the raised plantlets were found to produce microtubers in a period of 8 to 10 weeks”
t0“potato (Solanum tuberosum L.)”
Claim · model-written summary
2.45 µM IBA induced the highest root number.
- Taxon
- potato (Solanum tuberosum L.)Solanum tuberosumt0
- Treatment
- 2.45 µM IBA in full strength basal MS mediume0
- Outcome
- number of rootse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2.45 µM IBA in full strength basal MS medium induced highest number of roots”
t0“potato (Solanum tuberosum L.)”
The same questions in other papers
Gibberellic acid · yield · Solanum tuberosum (potato)Results differ
10 papers in the same direction as this paper · 3 in the opposite direction
- positive
- no effect
- negative
10 papers more in this group
See all 14 papers →kinetin · growth · Solanum tuberosum (potato)Positive and negative results
11 papers in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- no effect
11 papers more in this group
See all 15 papers →2,4-D · growth · Solanum tuberosum (potato)Positive and negative results
15 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- positive
13 papers more in this group
See all 17 papers →indole-3-butyric acid · growth · Solanum tuberosum (potato)Results agree
10 papers in the same direction as this paper
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →N-benzyladenine · yield · Solanum tuberosum (potato)Results agree
11 papers in the same direction as this paper
- positive
- positive
- positive
8 papers more in this group
See all 12 papers →in vitro · growth · Solanum tuberosum (potato)Positive and negative results
68 papers in the same direction as this paper · 12 in the opposite direction · 1 reporting no difference from an alternative
- positive
- negative
- no effect
78 papers more in this group
See all 82 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.