Abstract
SUMMARY Inoculation at 0 °C improved the efficiency of infecting tomato protoplasts with RNA of tobacco mosaic virus (TMV). Up to 41% infection was obtained when inoculation was at 0 °C with 10 µg/ml RNA in the presence of 1 µg/ml poly-d-lysine in 0.01 m-potassium citrate buffered 0.7 m-mannitol (pH 5.2). The homozygous gene for resistance Tm-1 was expressed in tomato protoplasts inoculated with RNA of TMV-L, a common tomato strain of TMV; no virus progeny were detected by fluorescent antibody staining or infectivity assay. Virus multiplied rapidly in protoplasts from susceptible homozygotes. Protoplasts homozygous for Tm-1 were infected by the RNA of TMV-CH2, a tomato strain which can overcome this resistance in plants. These results resemble those previously reported for inocula using intact virus, and suggest that Tm-1 blocks virus growth after the uncoating stage.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Inoculation at 0 °C improved TMV RNA infection efficiency in tomato protoplasts.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- Inoculation at 0 °Ce0
- Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Outcome
- efficiency of infecting tomato protoplastse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation at 0 °C improved the efficiency of infecting tomato protoplasts with RNA of tobacco mosaic virus (TMV).”
Claim · model-written summary
Infection reached up to 41% at 0 °C with the specified RNA, poly-d-lysine and buffer conditions.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- inoculation was at 0 °C with 10 µg/ml RNA in the presence of 1 µg/ml poly-d-lysine in 0.01 m-potassium citrate buffered 0.7 m-mannitol (pH 5.2)e0
- Interactor
- Tobacco Mosaic VirusTobacco mosaic virust0pathogen
- Outcome
- infectione0
- Value
- 41%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Up to 41% infection was obtained when inoculation was at 0 °C with 10 µg/ml RNA in the presence of 1 µg/ml poly-d-lysine in 0.01 m-potassium citrate buffered 0.7 m-mannitol (pH 5.2).”
t0“Tomato Protoplasts with Tobacco Mosaic Virus RNA”
Claim · model-written summary
Tm-1 homozygous tomato protoplasts yielded no detectable TMV-L progeny after RNA inoculation.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- inoculated with RNA of TMV-Le0
- Interactor
- TMV-Le0pathogen
- Gene
- Tm-1e0
- Outcome
- no virus progeny were detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The homozygous gene for resistance Tm-1 was expressed in tomato protoplasts inoculated with RNA of TMV-L, a common tomato strain of TMV; no virus progeny were detected by fluorescent antibody staining or infectivity assay.”
Claim · model-written summary
TMV multiplied rapidly in protoplasts from susceptible homozygotes.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- susceptible homozygotese0
- Interactor
- Tobacco Mosaic VirusTobacco mosaic virust0pathogen
- Outcome
- Virus multiplied rapidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus multiplied rapidly in protoplasts from susceptible homozygotes.”
t0“Tomato Protoplasts with Tobacco Mosaic Virus RNA”
Claim · model-written summary
TMV-CH2 RNA infected protoplasts homozygous for Tm-1.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- TMV-CH2e0pathogen
- Gene
- Tm-1e0
- Outcome
- were infectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protoplasts homozygous for Tm-1 were infected by the RNA of TMV-CH2, a tomato strain which can overcome this resistance in plants.”
Claim · model-written summary
The results suggest Tm-1 blocks TMV growth after uncoating.
- Taxon
- TomatoSolanum lycopersicumt0
- Interactor
- Tobacco Mosaic VirusTobacco mosaic virust0pathogen
- Gene
- Tm-1e0
- Outcome
- blocks virus growthe0
- Qualifier
- after the uncoating stagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggest that Tm-1 blocks virus growth after the uncoating stage”
t0“Tomato Protoplasts with Tobacco Mosaic Virus RNA”
The same questions in other papers
inoculation · Tobacco mosaic virus · Solanum lycopersicum (tomato)Positive and negative results
9 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.