Abstract
OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa). It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidopsis thaliana) and rice, respectively. Expression of OsbZIP46 was strongly induced by drought, heat, hydrogen peroxide, and abscisic acid (ABA) treatment; however, it was not induced by salt and cold stresses. Overexpression of the native OsbZIP46 gene increased ABA sensitivity but had no positive effect on drought resistance. The activation domain of OsbZIP46 was defined by a series of deletions, and a region (domain D) was identified as having a negative effect on the activation. We produced a constitutive active form of OsbZIP46 (OsbZIP46CA1) with a deletion of domain D. Overexpression of OsbZIP46CA1 in rice significantly increased tolerance to drought and osmotic stresses. Gene chip analysis of the two overexpressors (native OsbZIP46 and the constitutive active form OsbZIP46CA1) revealed that a large number of stress-related genes, many of them predicted to be downstream genes of ABF/AREBs, were activated in the OsbZIP46CA1 overexpressor but not (even down-regulated) in the OsbZIP46 overexpressor. OsbZIP46 can interact with homologs of SnRK2 protein kinases that phosphorylate ABFs in Arabidopsis. These results suggest that OsbZIP46 is a positive regulator of ABA signaling and drought stress tolerance of rice depending on its activation. The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of OsbZIP46CA1 in genetic engineering of drought tolerance.
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Claims 10
Claim · model-written summary
OsbZIP46 expression was induced by drought, heat, peroxide and ABA.
- Taxon
- Rice
Oryza sativat0 - Treatment
- drought, heat, hydrogen peroxide, and abscisic acid (ABA) treatmente0
- Gene
- OsbZIP46e0
- Outcome
- Expression of OsbZIP46e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsbZIP46 was strongly induced by drought, heat, hydrogen peroxide, and abscisic acid (ABA) treatment”
t0“Rice”
Claim · model-written summary
Salt and cold did not induce OsbZIP46 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- salt and cold stressese0
- Gene
- OsbZIP46e1
- Outcome
- Expression of OsbZIP46e1
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was not induced by salt and cold stresses”
e1“Expression of OsbZIP46”
t0“Rice”
Claim · model-written summary
Native OsbZIP46 overexpression increased ABA sensitivity.
- Taxon
- RiceOryza sativat0
- Treatment
- Overexpression of the native OsbZIP46 genee0
- Gene
- OsbZIP46e0
- Outcome
- ABA sensitivitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the native OsbZIP46 gene increased ABA sensitivity but had no positive effect on drought resistance”
t0“Rice”
Claim · model-written summary
Native OsbZIP46 overexpression gave no positive drought-resistance effect.
- Taxon
- RiceOryza sativat0
- Treatment
- Overexpression of the native OsbZIP46 genee0
- Gene
- OsbZIP46e0
- Outcome
- drought resistancee0
- Direction
- no positive effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the native OsbZIP46 gene increased ABA sensitivity but had no positive effect on drought resistance”
t0“Rice”
Claim · model-written summary
Domain D negatively affected activation.
- Taxon
- RiceOryza sativat0
- Gene
- domain De0
- Outcome
- activatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a region (domain D) was identified as having a negative effect on the activation”
t0“Rice”
Claim · model-written summary
OsbZIP46CA1 overexpression increased drought and osmotic tolerance.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of OsbZIP46CA1e0
- Gene
- OsbZIP46CA1e0
- Outcome
- tolerance to drought and osmotic stressese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsbZIP46CA1 in rice significantly increased tolerance to drought and osmotic stresses”
Claim · model-written summary
Stress-related genes were activated in OsbZIP46CA1 overexpressors.
- Taxon
- RiceOryza sativat0
- Gene
- OsbZIP46CA1e0
- Outcome
- stress-related genese0
- Direction
- activatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress-related genes, many of them predicted to be downstream genes of ABF/AREBs, were activated in the OsbZIP46CA1 overexpressor”
t0“Rice”
Claim · model-written summary
These genes were not activated, and sometimes down-regulated, in native overexpressors.
- Taxon
- RiceOryza sativat0
- Gene
- OsbZIP46e0
- Outcome
- stress-related genese1
- Direction
- down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not (even down-regulated) in the OsbZIP46 overexpressor”
e1“large number of stress-related genes”
t0“Rice”
Claim · model-written summary
OsbZIP46 interacted with SnRK2 kinase homologs.
- Taxon
- RiceOryza sativat0
- Gene
- OsbZIP46e0
- Outcome
- interact with homologs of SnRK2 protein kinasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsbZIP46 can interact with homologs of SnRK2 protein kinases”
t0“Rice”
Claim · model-written summary
Activated stress genes differed from those activated by other rice homologs.
- Taxon
- riceOryza sativae0
- Gene
- OsbZIP46CA1e0
- Outcome
- stress-related genese0
- Comparator
- other rice ABF/AREB homologse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23)”
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