Abstract
Epigenetic states and certain environmental responses in mammals and seed plants can persist in the next sexual generation. These transgenerational effects have potential adaptative significance as well as medical and agronomic ramifications. Recent evidence suggests that some abiotic and biotic stress responses of plants are transgenerational. For example, viral infection of tobacco plants and exposure of Arabidopsis thaliana plants to UVC and flagellin can induce transgenerational increases in homologous recombination frequency (HRF). Here we show that exposure of Arabidopsis plants to stresses, including salt, UVC, cold, heat and flood, resulted in a higher HRF, increased global genome methylation, and higher tolerance to stress in the untreated progeny. This transgenerational effect did not, however, persist in successive generations. Treatment of the progeny of stressed plants with 5-azacytidine was shown to decrease global genomic methylation and enhance stress tolerance. Dicer-like (DCL) 2 and DCL3 encode Dicer activities important for small RNA-dependent gene silencing. Stress-induced HRF and DNA methylation were impaired in dcl2 and dcl3 deficiency mutants, while in dcl2 mutants, only stress-induced stress tolerance was impaired. Our results are consistent with the hypothesis that stress-induced transgenerational responses in Arabidopsis depend on altered DNA methylation and smRNA silencing pathways.
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Claims 11
Claim · model-written summary
Parental stress increased homologous recombination frequency in untreated Arabidopsis progeny.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- salt, UVC, cold, heat and floode0
- Outcome
- HRFe0
- Direction
- highere0
- Comparator
- untreated progenye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exposure of Arabidopsis plants to stresses, including salt, UVC, cold, heat and flood, resulted in a higher HRF, increased global genome methylation, and higher tolerance to stress in the untreated progeny.”
t0“Arabidopsis”
Claim · model-written summary
Parental stress increased global genome methylation in Arabidopsis progeny.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- salt, UVC, cold, heat and floode0
- Outcome
- global genome methylatione0
- Direction
- increasede0
- Comparator
- untreated progenye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exposure of Arabidopsis plants to stresses, including salt, UVC, cold, heat and flood, resulted in a higher HRF, increased global genome methylation, and higher tolerance to stress in the untreated progeny.”
t0“Arabidopsis”
Claim · model-written summary
Parental stress increased stress tolerance of untreated Arabidopsis progeny.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- salt, UVC, cold, heat and floode0
- Outcome
- tolerance to stresse0
- Direction
- highere0
- Comparator
- untreated progenye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exposure of Arabidopsis plants to stresses, including salt, UVC, cold, heat and flood, resulted in a higher HRF, increased global genome methylation, and higher tolerance to stress in the untreated progeny.”
t0“Arabidopsis”
Claim · model-written summary
The transgenerational stress effect did not persist across successive Arabidopsis generations.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- successive generationse0
- Outcome
- transgenerational effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This transgenerational effect did not, however, persist in successive generations.”
t0“Arabidopsis”
Claim · model-written summary
5-azacytidine treatment decreased genomic methylation in progeny of stressed Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 5-azacytidinee0
- Outcome
- global genomic methylatione0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of the progeny of stressed plants with 5-azacytidine was shown to decrease global genomic methylation and enhance stress tolerance.”
t0“Arabidopsis”
Claim · model-written summary
5-azacytidine treatment enhanced stress tolerance in progeny of stressed Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 5-azacytidinee0
- Outcome
- stress tolerancee0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of the progeny of stressed plants with 5-azacytidine was shown to decrease global genomic methylation and enhance stress tolerance.”
t0“Arabidopsis”
Claim · model-written summary
Stress-induced HRF was impaired in Arabidopsis dcl2 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dcl2e0
- Outcome
- Stress-induced HRFe0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stress-induced HRF and DNA methylation were impaired in dcl2 and dcl3 deficiency mutants”
t0“Arabidopsis”
Claim · model-written summary
Stress-induced DNA methylation was impaired in Arabidopsis dcl2 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dcl2e0
- Outcome
- DNA methylatione0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stress-induced HRF and DNA methylation were impaired in dcl2 and dcl3 deficiency mutants”
t0“Arabidopsis”
Claim · model-written summary
Stress-induced HRF was impaired in Arabidopsis dcl3 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dcl3e0
- Outcome
- Stress-induced HRFe0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stress-induced HRF and DNA methylation were impaired in dcl2 and dcl3 deficiency mutants”
t0“Arabidopsis”
Claim · model-written summary
Stress-induced DNA methylation was impaired in Arabidopsis dcl3 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dcl3e0
- Outcome
- DNA methylatione0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stress-induced HRF and DNA methylation were impaired in dcl2 and dcl3 deficiency mutants”
t0“Arabidopsis”
Claim · model-written summary
Stress-induced tolerance was impaired only in Arabidopsis dcl2 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- dcl2e0
- Outcome
- stress-induced stress tolerancee0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while in dcl2 mutants, only stress-induced stress tolerance was impaired.”
t0“Arabidopsis”
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.