Abstract
Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth occurred when ammonium salts of shikimate, tartrate, acetate, carbonate, or sulfate were used. The cells also grew well with l-glutamine as nitrogen source. The specific activities of glutamine synthetase and isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate) were lower than in cells grown on a nitrate medium, but ammonium enhanced the activity of glutamate dehydrogenase. Cells of soybean, wheat, and flax have been cultured for an extended period on the ammonium citrate medium.
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Claims 7
Claim · model-written summary
Soybean cells grew on ammonium when Krebs cycle acids were added.
- Taxon
- Soybean
Glycine maxe0 - Treatment
- ammonium as the sole nitrogen sourcee0
- Outcome
- grewe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added”
Claim · model-written summary
Citrate, malate, fumarate and succinate ammonium salts supported satisfactory growth.
- Treatment
- ammonium salts of citrate, malate, fumarate, or succinatee0
- Outcome
- Satisfactory growthe0
- Comparator
- regular medium containing nitrate and ammoniume0
Not reportedTaxonDirection
Verbatim
e0“Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium”
Claim · model-written summary
Shikimate, tartrate and acetate ammonium salts supported little or no growth.
- Treatment
- ammonium salts of shikimate, tartrate, acetate, carbonate, or sulfatee0
- Outcome
- Little or no growthe0
Not reportedTaxonComparatorDirection
Verbatim
e0“Little or no growth occurred when ammonium salts of shikimate, tartrate, acetate, carbonate, or sulfate were used”
Claim · model-written summary
Cells grew well with l-glutamine.
Not reportedTaxonComparatorDirection
Verbatim
e0“The cells also grew well with l-glutamine as nitrogen source”
Claim · model-written summary
Glutamine synthetase and isocitrate dehydrogenase specific activities were lower than on nitrate medium.
- Outcome
- specific activities of glutamine synthetase and isocitrate dehydrogenasee0
- Direction
- lowere0
- Comparator
- nitrate mediume0
Not reportedTaxonTreatment
Verbatim
e0“The specific activities of glutamine synthetase and isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate) were lower than in cells grown on a nitrate medium”
Claim · model-written summary
Ammonium enhanced glutamate dehydrogenase activity.
Not reportedTaxonComparator
Verbatim
e0“ammonium enhanced the activity of glutamate dehydrogenase”
Claim · model-written summary
Soybean, wheat and flax cells were cultured long term on ammonium citrate medium.
- Taxon
- soybeanGlycine maxe0
- Treatment
- ammonium citrate mediume0
- Outcome
- cultured for an extended periode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cells of soybean, wheat, and flax have been cultured for an extended period on the ammonium citrate medium”
The same questions in other papers
sulfate · growth · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- negative
acetate · growth · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- no effect
nitrogen · molecular: metabolites · Glycine max (soybean)Results differ
36 papers in the same direction as this paper · 42 in the opposite direction · 2 reporting no difference from an alternative
- negative
- no effect
- negative
77 papers more in this group
See all 81 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.