Abstract
Alternative oxidase activity in potato tuber (Solanum tuberosum L. cv Bintje) callus mitochondria with exogenous NAD(P)H as substrate is inhibited by low concentrations of the detergent Triton X-100. Alternative oxidase activity with succinate or malate as substrate is not affected by these low concentrations of Triton X-100. Cytochrome pathway activity was not influenced under these conditions, neither with endogenous nor with exogenous substrate. Washing of Triton X-100-treated mitochondria did partially restore both uninhibited and CN-resistant NADH oxidation, indicating that under these conditions Triton X-100 does not permanently remove major components from the mitochondrial membrane. Apparently, it is possible to manipulate mitochondria in such a way that the access of exogenous NADH to the alternative pathway is blocked while access to the cytochrome pathway is uninhibited. It is suggested that membrane conditions have a regulatory function (possibly via influencing the diffusion path) in the oxidation of exogenous NADH via the alternative pathway.
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Claims 6
Claim · model-written summary
Low Triton X-100 concentrations inhibit alternative oxidase activity with exogenous NAD(P)H.
- Taxon
- potato
Solanum tuberosume0 - Material
- cv Bintjee0
- Organ / stage
- tubere0
- Treatment
- low concentrations of the detergent Triton X-100e0
- Outcome
- Alternative oxidase activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alternative oxidase activity in potato tuber (Solanum tuberosum L. cv Bintje) callus mitochondria with exogenous NAD(P)H as substrate is inhibited by low concentrations of the detergent Triton X-100”
Claim · model-written summary
Low Triton X-100 concentrations do not affect alternative oxidase activity with succinate or malate.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- low concentrations of Triton X-100e0
- Outcome
- Alternative oxidase activity with succinate or malate as substratee0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alternative oxidase activity with succinate or malate as substrate is not affected by these low concentrations of Triton X-100”
t0“Potato Tuber Callus”
Claim · model-written summary
Triton X-100 did not influence cytochrome pathway activity with either endogenous or exogenous substrate.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- low concentrations of the detergent Triton X-100e1
- Outcome
- Cytochrome pathway activitye0
- Direction
- not influencede0
- Qualifier
- neither with endogenous nor with exogenous substratee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytochrome pathway activity was not influenced under these conditions, neither with endogenous nor with exogenous substrate”
e1“low concentrations of the detergent Triton X-100”
t0“Potato Tuber Callus”
Claim · model-written summary
Washing detergent-treated mitochondria partially restored uninhibited NADH oxidation.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- Washing of Triton X-100-treated mitochondriae0
- Outcome
- NADH oxidatione0
- Direction
- partially restoree0
- Qualifier
- uninhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Washing of Triton X-100-treated mitochondria did partially restore both uninhibited and CN-resistant NADH oxidation”
t0“Potato Tuber Callus”
Claim · model-written summary
Washing detergent-treated mitochondria partially restored CN-resistant NADH oxidation.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- Washing of Triton X-100-treated mitochondriae0
- Outcome
- CN-resistant NADH oxidatione0
- Direction
- partially restoree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Washing of Triton X-100-treated mitochondria did partially restore both uninhibited and CN-resistant NADH oxidation”
t0“Potato Tuber Callus”
Claim · model-written summary
The authors suggest membrane conditions regulate exogenous NADH oxidation through the alternative pathway, possibly through the diffusion path.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- oxidation of exogenous NADH via the alternative pathwaye0
- Qualifier
- membrane conditions have a regulatory function (possibly via influencing the diffusion path)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is suggested that membrane conditions have a regulatory function (possibly via influencing the diffusion path) in the oxidation of exogenous NADH via the alternative pathway”
t0“Potato Tuber Callus”
The same questions in other papers
processing · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- no effect
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.